Mammary carcinoma arising in an adenoma in a ewe

A large, firm, multi-cystic mammary gland mass grew slowly over 4 y in a 12-y-old, female Finn–Shetland cross sheep. A diagnosis of epithelial malignancy was suspected following fine-needle aspiration cytology at 30 mo after initial observation. The sheep was euthanized when the flock was downsized 18 mo later. A field postmortem examination revealed a large mammary mass, but an absence of metastases to internal organs. Imprint cytology of the mammary tissue supported a benign proliferative process. Histologically, mammary tissue was obliterated by cystic, tubular, and papillary adenomatous arrangements of mammary epithelium, with an anaplastic component, consistent with mammary carcinoma arising in an adenoma. IHC showed strong nuclear positivity to the antibody against progesterone receptor and minimal positivity to the antibody against estrogen receptor alpha expression. Intrinsic subtyping for basal or luminal epithelial origin was attempted through adaptation of companion animal IHC classification panels; high- and low-molecular-weight cytokeratins (CK5, CK8, CK18) failed to stain, but p63 expression for basal epithelium was positive.     

THORACIC METASTASES OF CANINE MAMMARY GLAND TUMORS

The absence or presence of metastases on thoracic radiographs in 55 female dogs with mammary gland tumors was assessed by comparing the results of a single radiographic examination, using dorsoventral and left lateral views, with clinical and histologic follow-up data. Radiographic abnormalities found in dogs with thoracic metastases were classified as well-defined pulmonary nodules, ill-defined pulmonary nodules, and involvement of pleural effusion. No obvious difference in the effect on the right or left lung lobes was found. The sensitivity, specificity and accuracy for the detection of thoracic metastases were 65%, 97%, and 87%, respectively.     

Suppurative Inflammation of Apocrine Sweat Glands (Suppurative Hidradenitis) in the Dog: A Retrospective Clinicopathological Analysis of 100 Cases

Résumé— Une analyse rétrospective clinicopathologique a été menée chez 100 chiens présentant une hydradénite suppurative diagnostiquée par biopsie cutanée. Aucune prédisposition d'âge, de race ou de sexe n'a été mise en évidence. Tous les chiens présentaient cliniquement une folliculite bactérienne, ou une furonculose, ou les deux, qu'elle soit primaire ou secondaire à d'autres dermatoses. Aucene lésion clinique n'était pathognomonique d'hidradénite suppurative. Chez 73 chiens, l'hidradénite suppurative était associée microscopiquement à des degrés variables d'inflammation du follicule pileux. Chez 27 chiens, l'hidradénite suppurative était la seule évidence histologique d'inflammation annexielle. L'observation histopathologique d'hidradénite suppurative suggère l'existence sur le plan clinique d'une folliculite bactérienne, d'une furonculose, ou des 2. [Scott, D.W. Suppurative inflammation of apocrine sweat glands (suppurative hidradentis) in the dog: a retrospective clinicopathological analysis of 100 cases (inflammation suppurative des glandes sudorales apocrines (hidradénite suppurative) chez le chien: une analyse rétrospective clinicopathologique de 100 cas). Resumen— Se llevó a cabo un estudio clinicopatológico retrospectivo en 100 perros con hidradenitis supurativa diagnosticada mediante biopsia cutánea. No se detectó predominancia alguna de raza, sexo o edad. A todes los perros se les habia diagnosticado clinicamente foliculitis o furunculosis bacteriana, o ambas, ya sea de forma primaria o secundaria a otras dermatosis. No se encontraron lesiones clinicas especificas indicativas de hidradenitis supurativa. En 73 animales, la hidradenitis supurativa estaba asociada con varios grados de inflamación folicular a nivel microscópico. En los 27 casos restantes, los anejos presentaban unicamente hidradenitis supurativa. La presencia de hidradenitis supurativa a nivel microscópico sugiere la existencia de foliculitis o furunculosis bacteriana clinicas, o de ambas. [Scott, D.W. Suppurative inflammation of apocrine sweat glands (suppurative hidradenitis) in the dog: a retrospective clinicopathological analysis of 100 cases (inflamación supurativa de las glándulas sudoriparas apocrinas (hidradenitis supurativa) en el perro: estudio clinicopatológico retrospectivo de 100 casos). Abstract— A retrospective clinicopathological study was conducted on 100 dogs with suppurative hidradenitis as determined by skin biopsy. No apparent age, breed, or sex predilections were recognized. All dogs had been given a clinical diagnosis of bacterial folliculitis, furunculosis, or both of these, whether primary or secondary to other dermatoses. There were no clinical lesions that uniquely suggested the presence of suppurative hidradenitis. In 73 dogs, suppurative hidradenitis occurred in conjunction with varying degrees of hair follicle inflammation microscopically. In 27 dogs, suppurative hidradenitis was the only histological evidence of adnexal inflammation. The histopathologic finding of suppurative hidradenitis suggests the existence of clinical bacterial folliculitis, furunculosis, or both of these.     

实验性流行性腹泻仔猪小肠粘膜上皮细胞及肠系膜淋巴结的超微结构

给8头生后3d的哺乳仔猪经口感染猪流行性腹泻病毒(PEDV)“吉”毒株,于感染后18、30、45和96h各扑杀2头,以透射电镜和扫描电镜观察了小肠粘膜上皮细胞及肠系膜淋巴结的超微结构。结果表明,小肠上皮细胞的病变因感染时间不同而有明显差异。上皮细胞的脱落和残留上皮细胞超微结构的破坏,以感染后30h最严重,病毒在这些上皮细胞内的增殖最显著。感染后45h,见有大量新生上皮细胞修补损伤的肠绒毛。感染后96h,小肠绒毛短缩、粗大乃至发生融合。实验仔猪肠系膜淋巴结内巨噬细胞和淋巴细胞的超微结构均遭到破坏,在巨噬细胞内见有PED冠状病毒粒子。     

Cytochemical staining characteristics of lymph nodes from normal and lymphoma-affected dogs

Frozen sections and imprint smears were used to evaluate the presence and pattern of cytochemical staining reactions in the B- and T-cell regions of lymph nodes from normal dogs and dogs with lymphoma. Staining procedures evaluated included peroxidase (PER), Sudan black B (SBB), naphthol AS-D chloroacetate esterase (CAE), alpha-naphthyl butyrate esterase (NBE), acid phosphatase (ACP), and leukocyte alkaline phosphatase (LAP). In normal lymph nodes, macrophages and some lymphocytes within the interfollicular (T-cell) region and medulla stained positive with ACP and NBE. Smaller numbers of macrophages also occurred sporadically within the germinal follicles. Cells positive for PER, SBB, and CAE were scattered infrequently throughout all regions of the normal lymph node, consistent with granulocytes and mast cells. The LAP stained cells were predominantly and prominently located within the mantle zone of secondary follicles and to a much lesser extent within the germinal centers, compatible with B-cell lymphocytes derived from follicular center cells. Of the 12 dogs with lymphoma, 7 cases (4 immunoblastic, 2 large noncleaved, 1 small noncleaved) stained diffusely positive with LAP, 4 cases (all lymphoblastic) had numerous focally positive lymphocytes using ACP and NBE, and 1 case (immunoblastic) did not stain positive with any of the cytochemical reactions. Cytochemical staining of canine lymph nodes with NBE, ACP, and LAP proved useful in distinguishing between B- or T-cell regions and detecting different cell types of canine lymphoma.     

Effect of Jiawei Sini Decoction on glucocorticoid receptor in thymocytes of chronic psychological stress rats

AIM: To observe the effect of Jiawei Sini Decoction (JWSND) on glucocorticoid receptor (GCR) in thymocytes of chronic psychological stress rats. METHODS: The rats were randomly divided into 4 groups, control group (C), model group (M), group treated by JWSND C₁, group treated by ginsenosides C₂. The number of thymocyte GCR sites and the GCR nuclear thanslocation rate were detected by radioimmunoassay. RESULTS: Compared with the control group, in the model group, the thymocyte weight index lowered significantly ( P<0.05 ), and the GCR nuclear thanslocation rate was increased significantly ( P<0.01 ), but the number of thymocyte GCR sites was unchanged. Compared with the model group, thymus gland weight indexes of C₁ and C₂ were increased significantly ( P<0.05 ), while the GCR nuclear thanslocation rate lowered significantly ( P<0.01 ). Moreover, no significant difference was found in all indexes between C₁ and control group. CONCLUSION: The inhibitory effect of glucocorticoid on the thymus could be significantly reversed by JWSND via suppressing the thanslocation of GCR from cytoplasm to nucleus in chronic psychological stress rats.     

Effects of riboflavin and ascorbic acid on apoptosis and proliferative inhibition of mouse thymocytes induced by deoxynivalenol in vivo

AIM: To explore the effects of riboflavin and ascorbic acid on the apoptosis induced by deoxynivalenol(DON) in mouse thymocytes. METHODS: The effects of riboflavin and ascorbic acid on the apoptosis and proliferation inhibition of thymocytes induced by DON in KM mice were studied with animal experiment, DNA agarose gel electrophoresis and flow cytometric DNA content analysis. RESULTS: Apoptosis rate of thymocytes in DON (4 mg/kg) treated group was13.73%±15.3% The percentages of apoptosis in riboflavin (1.25 mg/kg-10mg/kg) and ascorbic acid (25 mg/kg-100mg/kg) pretreated thymocytes groups were significantly lower than that in DON group (P <0.05). The result of DNA agarose gel electrophoresis showed that the characteristic ladder pattern of apoptosis was found in DON-treated thymocytes, but not in control and riboflavin pretreatment and ascorbic acid pretreatment groups The significant differences in proliferation index were not found among DON-treated thymocytes and riboflavin and ascorbic acid-pretreated thymocytes CONCLUSION: Pretreatment with riboflavin and ascorbic acid inhibit apoptosis of mouse thymocytes induced by DON in certain extent and have no effect on proliferation inhibition by DON.     

补铬对热应激蛋鸡肾上腺组织结构的影响

试验选择处于热应激条件下的192只35周龄新罗曼褐壳蛋鸡,随机分为6组,分别饲喂添加0.10,20,30,40,50 g/t含铬量为7.410mg/kg的葡萄糖耐量因子(GTF)的玉米—豆粕型基础日粮52d。试验结束时随机抽取试验鸡36只(每组6只),取肾上腺称重并制作切片,研究有机铬对热应激蛋鸡肾上腺组织结构的影响。结果试验组肾上腺重量较对照组略有增加;热应激时蛋鸡肾上腺的肾间组织比例增大,肾间组织和嗜铬组织的细胞均有结构受损的现象;补铬使肾间组织比例减小,嗜铬细胞增多,细胞结构完好。