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为了解东北地区大豆品种间大豆脂肪氧化酶(LOX)活性差异规律,整理适于东北地区种植的大豆种质资源307份(6份半野生品种,17份地方品种及4份国外品种,其余为栽培品种),统一种植,秋季收获后,测定各品种成熟籽粒脂肪氧化酶总活性。分析品种间脂肪氧化酶总活性分布规律、大豆脂肪氧化酶总活性与β-胡萝卜素漂白性之间关系。结果表明,307份大豆品种间脂肪氧化酶总活性差异极显著,平均值为21.68 U·mL~(-1),最小值为2.51 U·mL~(-1),最大值为42.38 U·mL~(-1)。根据脂肪氧化酶总活性差异,通过聚类分析将307份大豆品种分为3类。低活性品种占总量43.65%,脂肪氧化酶总活性平均值为15.25 U·mL~(-1);中等活性品种占总量43.97%,脂肪氧化酶活性平均值为24.64 U·mL~(-1);高活性品种占总量12.38%,脂肪氧化酶总活性平均值为33.87 U·mL~(-1)。脂肪氧化酶总活性与油分含量呈正相关,与蛋白含量呈负相关,与漂白性呈正相关。 相似文献
3.
Wen-wen LIU Min XIN Meng-ji CAO Meng QIN Hui LIU Shou-qi ZHAO Xi-feng WANG 《农业科学学报》2018,17(10):2281-2291
To identify the possible quarantine viruses in seven common sunflower varieties imported from the United States of America and the Netherlands, we tested total RNAs extracted from the leaf tissues using next-generation sequencing of small RNAs. After analysis of small RNA sequencing data, no any quarantine virus was found, but a double-stranded RNA(dsRNA) molecule showing typical genomic features of endornavirus was detected in two varieties, X3939 and SH1108. Full-length sequence and phylogenetic analysis showed that it is a novel endornavirus, temporarily named as Helianthus annuus alphaendornavirus(HaEV). Its full genome corresponds to a 14 662-bp dsRNA segment, including a 21-nt 5′ untranslated region(UTR), 3' UTR ending with the unique sequence CCCCCCCC and lacking a poly(A) tail. An open reading frame(ORF) that encodes a deduced 4 867 amino acids(aa) polyprotein with three domains: RdRP, Hel and UGT(UDP-glycosyltransferase). HaEV mainly distributed in the cytoplasm but less in the nucleus of leaf cells by fluorescence in situ hybridization(FISH) experiment. This virus has a high seed infection rate in the five varieties, X3907, X3939, A231, SH1108 and SR1320. To our knowledge, this is the first report about the virus of the family Endornaviridae in the common sunflower. 相似文献
4.
Establishing an eyeball-weight relationship for Litopenaeus vannamei using machine vision technology
This study aimed to establish a shrimp eyeball-weight relationship model for Litopenaeus vannamei using machine vision technology. A total of 295 shrimp were sampled from a recirculating aquaculture system (RAS). The long-axis length (d), body length (L), and body weight (W) of each individual was measured. The long axis length of the shrimp eyeball was identified and measured using machine vision technology. Continuous fitting and piecewise fitting models were used to construct the eyeball-weight relationship model for L. vannamei. The continuous fitting relationship model was described as: W = 38.865d2.7914, while the piecewise model was described as: d < 2 mm, W = 0.0326d3.7363, R² = 0.9288; 2 mm ≤ d < 3.9 mm, W = 0.0401d3.104, R² = 0.9629; 3.9 mm ≤ d < 5.8 mm, W = 0.0421d3.0311, R² = 0.9216; 5.8 mm < d, W = 0.103d2.6226, R² = 0.9457. The root mean square error (RMSE) of the piecewise fitting model (0.0244, 0.1575, 0.5034, 0.7072) was smaller than the continuous fitting model (0.8229). The correlation coefficient (R2) of the piecewise model (0.9288, 0.9629, 0.9216, and 0.9457) was similar to that of the continuous fitting model (R2 = 0.9621). The results indicated that the piecewise fitting model is suitable for calculating the biomass of L. vannamei in RAS and provides a novel way of estimating the biomass of L. vannamei cultured in RAS. The piecewise fitting model can also provide the foundation of evaluating the production of shrimp using underwater image recognition in intelligent aquaculture systems. 相似文献
5.
石灰性土壤的电超滤(EUF)特性主要是浸提时因需控制电流而必须大大降低电压。在一定范围内,降压程度与土壤 CaCO_3%有密切关系。控流限额愈低,电压需成比例地下降,但土壤EUF-值减小不多。电流控制在一定值时,离子的解吸总量亦为定值。控流主要控制了各石灰性土壤解吸/溶解份额特大的 Ca+Mg 量近于定值,对于含量小的其他养分离子则并无多大影响,仍可区辨各土壤间的差别。通过冬小麦盆栽试验和有关化学测试法等方面的相关研究,表明采用国际通用的5g 土样和控电流方法测定 EUF-K 和-P 等有效养分,对于石灰性土壤也是可行的,但其EUF-(Ca+Mg)值则毫无植物营养有效性的意义。土壤 EUF 值只是一种指数,并非真正的解吸离子量。石灰性土壤 EUF-K 值因浸提时降压控流而略低,80℃时5min 内解吸的钾不一定是非交换性钾。由于 Ca-P 和 CaCO_3在高温高电压时浸出较多,故石灰性土壤 EUF-P 和-(Ca+Mg)的80℃值/20℃值之比,与酸性土壤或非石灰性土壤相比要大得多。 相似文献
6.
Field experiments were conducted to determine the direct and residual contributions of legumes to the yield and nitrogen (N) uptake of maize during the wet seasons of 1994 and 1995 at the University Farm, Abubakar Tafawa Balewa University, Bauchi, Nigeria, located in the Northern Guinea savannah of Nigeria. Nodulating soybean, lablab, green gram and black gram contributed to the yield and N uptake of maize either intercropped with the legumes or grown after legumes as a sole crop. Direct transfer of N from the nodulating soybean, lablab, green gram and black gram to the intercropped maize was 24.9–28.1, 23.8–29.2, 19.7–22.1 and 18.4–18.6 kg N ha–1 , respectively. However, the transfer of residual N from these legumes to the succeeding maize crop was 18.4–20.0, 19.5–29.9, 12.0–13.7 and 9.3–10.3 kg N ha–1 , respectively. Four years of continuous lablab cropping resulted in yields and N uptake of the succeeding maize crop grown without fertilizer N that were comparable to the yields and N uptake of the succeeding maize crop supplied with 40–45 kg N ha–1 and grown after 4 years of continuous sorghum cropping. It may therefore be concluded that nodulating soybean, lablab, green gram and black gram may be either intercropped or grown in rotation with cereals in order to economize the use of fertilizer N for maize production in the Nigerian savannah. 相似文献
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通过PCR方法自含NDVZF基因的克隆质粒中扩增NDVF基因 ,将其与真核表达载体pcDNA3..1/V5_His_TOPO重组。经核酸内切酶酶切 ,阳性克隆鉴定准确无误 ,核酸序列测定结果与原始基因比较 ,同源性大于 99% ,启始密码和终止密码未出现变异 ,将该重组质粒命名为pcDNANDVZF。将pcDNANDVZF在脂质体作用下转染CEF细胞 ,用间接免疫荧光试验检测 ,结果证明pcDNANDVZF可在CEF细胞中大量表达F蛋白。 相似文献
9.
大豆室内耐冷筛选及其在田间的应用研究 总被引:4,自引:1,他引:3
本试验把生长箱大豆耐低温萌发筛选与田间早春播研究相结合,验证耐低温发芽的可靠性。在实验室,萌发期表现抗冷的大豆,田间出昔期仍表现抗冷或较抗冷。实验表明,大豆出苗的生物学最低温度平均为7.1℃,所需有效积温136℃。地温的高低直接影响大豆早春出苗的多寡,两者呈密切的正相关。黄、青、黑豆中,以黑豆抵抗早春不良气候条件的能力最强。 相似文献
10.
S. Leibovitch B. L. Ma Wanga E. Maloba D. L. Smith 《Journal of Agronomy and Crop Science》1992,169(3):209-215
Aspects of intensive management practices such as high-yielding cultivars, narrow-row spacings and fungicide treatments could potentially increase cereal yields in regions with a short crop-growing season and occasionally dry and hot weather. A field experiment was carried out at McGill University, Canada for three years (1987 to 1989) to test the effects of triadimefon (1-[4-chlorophenoxy]-3,3-dimethyl-1-[1H-1,2,4-triazol-1-yl]-2-butanone, or Bayleton, a trade name) fungicide (0 vs. 140 g a.i. ha−1 ) and row spacing (10 vs. 20 cm) on the yield components, yield and other agronomic traits (spike emergence, days to maturity, leaf disease and plant height) of spring barley ( Hordeum vulgare L.) cv. Cadette, Laurier and Leger. A cultivar by row spacing interaction resulted in a 11 to 13.5 % increase in grain yield due to narrow rows for Laurier in two out of the three years and up to 16 % for Leger in one year. Over the three years grain yields were on average increased 6 to 12 % due to use of the narrower row spacing. Fungicide application to barley at the early heading stage effectively controlled leaf diseases without influence on yield components or grain yield. Our results indicate that some components of intensive management such as narrow row spacing can be applicable in regions with a short crop-growing season. 相似文献