岩溶流域典型农业区水体氢氧同位素的空间异质性及形成机制

分别于平水期和枯水期采集了花溪河流域典型农业区地表水和地下水样品。利用氢氧同位素示踪技术,结合土地利用类型对研究区不同水体的补给来源、季节变化及主要影响过程进行了分析,并对不同水体氢氧同位素值进行了空间插值分析,同时对其形成机制进行了分析,阐明了不同土地利用类型影响下的主要水文过程。结果表明:(1)研究区不同水体的主要补给来源为当地大气降水,月亮湖水库受蒸发作用影响明显,地表水和地下水的δD和δ~(18)O整体上呈现平水期高于枯水期的特征。(2)地下水的δD和δ~(18)O在枯水期与平水期均呈现明显的空间分异性特征,西部水田/水库集中区富集,东部旱地集中区贫化,土地利用对研究区环境水文过程影响明显。该研究结果有助于了解不同土地利用方式下地表水对地下水的影响,为流域管理提供科学依据。     

鸡流行性腹泻病原的分离鉴定及特性研究

用胰蛋白酶处理发病鸡的粪便和肠内容物，接种Marc145细胞，盲传数代后，从山东不同地区发生流行性腹泻的鸡群中分离到轮状病毒，并对分离的轮状病毒的生物学特性和理化特性进行了部分研究。结果表明病毒粒子的形态呈车轮状、大小为70-80nm；其病毒基因组的电泳图谱为5：1：3：2；病毒在Marc145细胞上传到第9代时的TCID50为10^-4.62／0．1mL；该分离株病毒对氯仿、乙醚有抵抗力；对pH3．0处理60min稳定；50℃ 30min能使其感染力下降10^2；1mol／L MgCl2不能增强其对50℃ 60min的抵抗力。动物回归试验中接种两周龄SPF鸡，24h后陆续发病，表现为持续性水样腹泻，与自然发病相同；剖检可见病鸡脱水、小肠内有大量的液体和气泡、肠粘膜变薄；组织学变化为肠绒毛上皮坏死、脱落，绒毛平均长度减少而隐窝深度增加，固有层中淋巴细胞浸润。其临床症状及病理组织学变化与自然发病相同。因此确定发生在山东鸡流行件腹泻的病原为轮状病毒．     

火鹤花衰退病诊断与检疫

本文描述了火鹤衰退病病害症状和2种穿孔线虫(香蕉穿孔线虫Rodopholus sim-ilis和柑橘穿孔线虫R．citrophilus)的鉴定特征，提出了检疫检验和疫情处理方法。     

Periodicity and gradients in dispersal ofAlternaria linicola in linseed crops

Conidia ofAlternaria linicola produced on infected linseed crops were mainly dispersed by wind. The numbers of conidia in the air above linseed crops collected by a Burkard spore sampler were greatest between 1200 h and 1300 h, when the relative humidity was lowest. Although numbers of conidia collected decreased with increasing height within and above the crop canopy, air-borneA. linicola conidia were present up to 80 cm above the crop canopy. Conidia ofA. linicola were transported by wind up to at least 40 m downwind from an artificial line inoculum source, but their numbers decreased with increasing distance from the source. In 1991, 1992, and 1993, the dispersal ofA. linicola conidia above linseed crops followed a seasonal periodicity which was influenced by weather conditions and cultural practices. The greatest numbers of conidia were collected during July, August and early September and coincided with periods favourable for sporulation and with an increase in the incidence of the disease in the senescent crop. Air-borneA. linicola conidia produced on point or line inoculum sources (naturally infected linseed stem debris) were responsible for the spread of the disease in linseed crops. In 1992 and 1993, the disease was first detected downwind from the sources, but by the end of the growing seasons, it had spread in all directions and up to 20 m and 60 m from the sources, respectively. Disease gradients were initially steep near the inoculum sources but they became flatter with time due to the secondary spread of the disease.     

山区农业面源污染的防治途径及对策

结合重庆市地形地貌特点和山区农业面源污染发生的因子，提出山区农业面源污染的防治对策。一方面必需结合当地的自然环境条件和经济发展水平提出农业面源污染的防治规划；另一方面必需结合生态农业建设这一主线，使物质、能量在生态系统内良性循环。紧紧抓住产生农业面源污染源的因子——化肥、农药、农田秸杆、畜禽粪便、农村生活废物等，提出科学的防治和减少途径。     

Alternative Route of Infection for Bacterial Seedling Blight of Rice Caused by Burkholderia plantarii

Burkholderia plantarii , the pathogen of bacterial seedling blight of rice, was detected in paddy water. Its concentration rose in July and August. The bacterial concentration in the paddy water was always higher along levees than at distances more than 5 m from levees. Confirmed to be released into water when graminaceous weeds were immersed, B. plantarii survived for at least 4 days at 30°C. B. plantarii was splashed at least 30 cm upward by rain splash in the field. Harvested seeds, which had been sprayed with B. plantarii released from graminaceous weeds at the flowering stage, retained the bacteria. Bacterial seedling blight occurred when the seeds were then sown in nursery boxes. These results indicated that graminaceous weeds growing on levees of paddy fields are a source of infection of the disease and that rice seeds are infected through the paddy water. Received 23 May 2002/ Accepted in revised form 1 September 2002     

茭白纹枯病发生规律及产量损失测定

茭白纹枯病发生规律与产量损失的研究结果表明:高温高湿、种植过密、偏施氮肥、缺乏钾素是病害加重的主要原因;田间发病盛期在7月中旬至8月中旬;单茭重减低率与病级之间呈极显著线性相关;始病期越早,危害越重,产量损失也越大;病害危害程度与有效分蘖数(孕茭数)减少率、单茭重减低率、产量损失率之间呈高度正相关。在产量损失构成中,有效分蘖数的减少为主要因素。     

不同碳源对虎奶菇菌核蛋白质营养价值的影响

测定了四种碳源对虎奶菇菌核蛋白质营养价值的影响。结果表明，以玉米淀粉为碳源，虎奶薅菌蛋白质的化学评分，必需氨基酸指数，生物价和营养指数分别为11．63、69．80、64．40和9．98，均居四种参试碳源的第一位；氨基酸评分和氨基酸比值系数分分别为14．55和12．45，均居四种参试碳源的第二位，这些结果证实，以玉米淀粉为碳源，虎奶菇菌核蛋白质营养价值高，值得推广应用。     

节能日光温室中番茄灰霉病发生规律的研究

通过 3年对节能日光温室环境温湿度监测、病菌孢子捕捉和番茄灰霉病发生规律研究明确 ,一般年份 ,灰霉病在番茄叶片上表现为明显的始发期、盛发期和末发期等 3个阶段 ;果实发病后 ,即进入盛发期。温室中病菌的发生与病害呈正相关 ,病菌的高峰期较病害的高峰期提前10～15d。持续的低温、高湿、苗期带病等是引起番茄灰霉病发生的重要因子。提出要从优化设施环境等方面来控制番茄灰霉病的危害。