家养林麝电刺激采精及精液品质研究

用电刺激采精法对20头次家养林麝进行电刺激采精及精液品质的分析。结果表明：电刺激射精电压为（4．6±0．5982）V;电刺激至射精的时间为（9．6667±6．3152）min;采精量为（0．369±0．242）mL;pH值为6．475±0．2552;精子密度为（6．935±7．0173）亿／mL;精子活力平均为0．67±0．1081。电刺激采精麻醉保定药品用量,氯胺酮（24．5075±10．0285）mg/kg体重,或陆眠宁（0．0225±0．005）mL/kg体重,均能达到满意的保定效果。     

乌苏里貉电刺激采精技术

1996～ 2 0 0 3年在乌苏里貉配种期对 33只雄貉用地西泮和盐酸氯胺酮复合麻醉后 ,进行了 70次电刺激采精试验 ,结果成功 6 8次 ,成功率 97.1% ,采精量平均为 (1.5 5± 1.35 )mL ,精液品质优良。采精后 ,没有发现雄貉有不良反应。     

梅花鹿电刺激采精技术研究

为提高鹿产品质量,发展优良鹿群数量,扩大优良基因在时间和地域上的利用率,2004年11月份在梅花鹿配种期对5头雄性梅花鹿进行了5次电刺激采精试验,成功5次,成功率100%,采得的精液品质优良,采精量为0.4~2.0 mL,平均采精量为1.28 mL,精子密度为5~10亿个/mL,活力为0.4~0.6,采精后雄鹿食欲、精神正常。     

Semen collection by urethral catheterization and electro-ejaculation with different voltages,and the effect of holding temperature and cooling rate before cryopreservation on semen quality in the Japanese macaque (Macaca fuscata)

In the Japanese macaque, semen has been collected by electro-ejaculation (EE), using the higher voltage stimuli compared to other species including genus Macaca. Semen coagulates immediately after ejaculation, which makes difficult to produce high-quality semen for artificial insemination. Recently, semen collection using urethral catheterization (UC) has been reported in carnivore and this technique may allow semen collection without coagulation in a less invasive manner. Further, the temporal preservation temperature and cooling rate of semen during cryopreservation affect post thawing sperm quality. In this study, to improve semen quality and quantity, as well as the animal welfare, semen collection was performed by EE with high (5–15 V) or low (3–6 V) voltage, UC and a combination of the two (EE-UC). It has been suggested that a high voltage is necessary for semen collection, but 10 V stimulation was effective enough and 15 V is for additional sperm collection. Also, liquid semen was collected by EE-UC and this could increase the total number of sperm. Further, to improve the post thawing sperm motility, semen was kept at four temperatures (4, 15, 25 and 37°C) for 60 min, and processed with two cooling procedures (slow cooling before second dilution and fast cooling after second dilution). Holding semen at 25°C and fast cooling after the second dilution maintained progressive motile sperm rate. The present results will contribute to the improvement of semen collection and animal welfare of Japanese macaques.