甲基对硫磷人工抗原的合成与鉴定

根据甲基对硫磷的结构特征,分别从甲基对硫磷的硝基一端或磷酸酯基甲氧基一端引入一定长度的间隔臂和活性基团,设计并合成了4种结构的半抗原;针对各种半抗原所带活性基团的性质,通过多种方法制备了与不同的载体蛋白偶联的人工抗原。进行动物免疫后,得到了针对甲基对硫磷的高效价抗体。     

The Generalized Conjugate Direction Method for Constrained Optimization A Geometrical Approech

The conjugate direction method for solving the unconstrained optimization problem is extended to solving the constrained optimization problem by method of differential geomtry.By inducing a new class of affine connections on a constrained sub-manifold, the primary constrched optilnhation problem is converted to a unconstrained local quadratic programming problem.Based on the definition and construction of a new class of generalized conjugate directions, it isproved that optimum value of the primary constrained optimization problem must be located on thegeodesic line which is formed by the conjugate directions mentioned above and can be reached withinfinite searching step. Therefore a new curve search algorithm with generalized conjugate directions isput forward.     

直线共轭内啮合齿轮泵齿轮接触强度的有限元分析

针对采用传统经验公式分析计算齿轮泵齿轮接触强度存在计算过程相当复杂、繁琐,误差较大的问题.在分析齿轮受力情况基础上,采用有限元法对直线共轭内啮合齿轮泵齿轮接触强度进行计算,并与经验公式计算的接触强度进行比较分析.结果表明,两种方法计算结果基本吻合,证实了采用有限元法分析计算的可行性与优越性,为直线共轭内啮合齿轮泵齿轮的设计、选材提供了一种新的方法,具有一定的工程应用价值.     

罗茨鼓风机叶轮数学模型的建立

罗茨鼓风机叶轮的传统设计方法是图解法,这种方法误差较大,浪费时间,在加工过程中需要反复进行修改,而且叶轮运转时容易产生噪声,效率低.针对这一问题,根据啮合原理中求解共轭曲线的解析法来求解叶轮的共轭曲线并构建精确的数学模型,以缩短叶轮的设计时间,提高叶轮的设计效率和设计品质.     

单变量动态Gamma分布模型及贝叶斯预测

给出了观测值服从单变量Gamma分布,并在自然参数与状态参数之间满足线性关系ωt=F'tθt的假设下,利用共轭分布给出了相应模型的修正递推及其预测公式.     

应用自动微分技术的CF—PCG方法及其效率分析

CF－PCG算法是牛顿法和预优共轭梯度法结合起来求解牛顿方程的一种非精确牛顿法。笔者将自动微分技术应用到该算法中，并证明应用自动微分技术的CF－PCG方法具有更高的效率。     

石房蛤毒素单克隆抗体的制备及其特性研究

将石房蛤毒素（Saxitoxin,STX）与牛血清白蛋白(BSA)偶联构建完全抗原STX-BSA,免疫BALB/c小鼠,通过杂交瘤技术获得1株稳定分泌抗STX单克隆抗体的杂交瘤细胞株,单抗亚类为IgM。体内诱生法收集腹水单抗,间接ELISA方法测定抗体效价为1∶102400,抗体亲和常数为3.71×106 L/mol。     

半立方抛物线形明渠共轭水深的迭代算法

【目的】寻求半立方抛物线形明渠共轭水深的迭代计算方法。【方法】根据半立方抛物线形明渠断面的几何形态及棱柱体水平明渠水跃方程,推求得到半立方抛物线形明渠共轭水深的迭代计算公式,并从理论上证明其收敛性;通过对工程中不同流量Q与不同断面形状参数p多种组合情况下的共轭水深进行计算和趋势线拟合,建立计算共轭水深迭代初值的直接计算式。【结果】推导出半立方抛物线形渠道断面的水跃方程,并进而得到跃前水深、跃后水深的迭代计算公式,运用迭代初值直接计算式求出迭代初值,将该值代入共轭水深迭代计算公式,经过几步迭代便可收敛得到精度很高的共轭水深值。【结论】推求的半立方抛物线形明渠共轭水深迭代计算公式物理概念明确、计算简捷、精度高、适用范围广,可以满足工程实践要求。     

Utilization of protein-A in immuno-histochemical techniques for detection of Peste des Petits Ruminants (PPR) virus antigens in tissues of experimentally infected goats

This paper constitutes the first record of utilizing the S. aureus protein-A (PA), conjugated to peroxidase enzyme, for the detection of the Peste des Petits Ruminants (PPR) virus antigens in tissues of experimentally infected goats. The goats were experimentally infected with a virulent PPR virus, which was previously isolated from a severe natural disease outbreak in gazelles, during 2002 in Saudi Arabia. The technique is rapid, and has the superiority over the peroxidase –anti-peroxidase (PAP) test in that, inactivation of the indigenous peroxidase in the tissues is not required and that it can be used against a wide range of animal species. An advantage over the other immunolabelled conjugates is that PA attaches specifically to the crystalizable fraction (Fc) of the IgG molecule, thus allowing the antigen binding fraction (Fab) of the molecule, free to interact specifically with the antigen. So, it doesn't actually compete with the antigen for the Fab portion of the IgG molecule. In the present study, PA conjugate detected the PPR virus antigens in various tissues of the experimentally infected goats.