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排序方式: 共有305条查询结果,搜索用时 46 毫秒
1.
Tayita Suttirojpattana Theesit Juanpanich Rangsun Parnpai Teraporn Vutyavanich 《Animal Science Journal》2021,92(1):e13585
Two-cell stage and blastocyst stage mouse embryos were equilibrated in a medium containing 7.5% ethylene glycol (EG) and 7.5% dimethyl sulfoxide (DMSO) for 8–15 min. Vitrification was performed in a medium containing 0.5 M sucrose and either 15% EG + 15% DMSO, 17.5% EG + 17.5% DMSO, or 20% EG + 20% DMSO for 30 s. They were then placed either on a hemi-straw (HS) or a hollow fiber vitrification (HFV) device and vitrified by cooled air inside a 0.5-ml straw. In two-cell embryos, a 100% survival rate was obtained from all groups except the 20% HS group (P > .05). All vitrified two-cell groups showed similar rates of blastocyst development to that of fresh control group (P > .05), except 17.5% and 20% HFV groups, which were significantly lower than the other groups (P < .05). In the blastocyst embryos, the HFV groups were divided into two subgroups (non-collapsed; HFV-NC and collapsed; HFV-C blastocyst). Re-expansion rate in 15% HFV-NC, 17.5% HFV-NC, and 15% HFV-C groups was reduced (P < .05), whereas the rest were similar to control. In conclusion, we established a simplified, reliable, and closed system for HFV vitrification applying hemi-straw, which does not require skilled practitioners. 相似文献
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Masahiro SATOH 《Animal Science Journal》2004,75(2):97-102
The present study evaluated the advantage of mixed‐model techniques over a selection index under different magnitudes of an additional systematic environmental effect (ASEE) in terms of accuracy of prediction and expected genetic gain. The data attempted to simulate a closed herd in a pig breeding program. The base population (G0) consisted of 10 males and 50 females. Six generations (G0 to G5) were selected by using a selection index of three traits without overlapping. Additional systematic environmental constants with four levels in a generation were assigned from a uniform distribution at different ranges. Breeding values of animals in the last generation (G5) were estimated on the basis of an index of individual phenotype (SI‐U), SI‐U adjusted for ASEE using a least‐squares mean (SI‐A), best linear unbiased prediction using an animal model excluding ASEE (AM‐E), and an animal model including ASEE (AM‐I). Accuracy of prediction and expected genetic gain were larger by the animal model than by the selection index, even if heritability of the traits selected was high and ASEE was set to zero. When ASEE was zero, the accuracy of prediction and expected genetic gain given by SI‐U and AM‐I were similar to those given by SI‐A and AM‐E, respectively. However, the differences in accuracy and expected gain between SI‐U and AI‐A and between AM‐I and AM‐E increased as the range of ASEE increased. It was concluded that selection based on an animal model was more effective than index selection, even if the herd environment was uniform and traits with high heritability were selected, and that it should be always included in an evaluation model, however slight any systematic environmental effect may be in a closed herd. 相似文献
4.
Improving management for higher reproduction accelerates genetic improvement in closed herd of swine
Masahiro SATOH 《Animal Science Journal》2004,75(6):499-502
The present study compared responses to selection at different conception rates and litter sizes at weaning in a simulated closed herd in a swine breeding program. The base population consisted of 10 males and 50 females, and 10 generations of selection was practiced by using individual phenotype or best linear unbiased prediction of breeding values for a trait with heritability (h2) of either 0.2 or 0.5. The probability of conception in a single mating was assumed to be 0.8, 0.9 or 1.0. Litter size at weaning was sampled randomly from a normal distribution with mean 8, 10 or 12 and variance 8.1225. Genetic response increased by approximately 6% for h2 = 0.2 and approximately 5% for h2 = 0.5 at generation 10 when conception rate was increased from 0.8 to 1.0. However, litter size at weaning did not affect response to selection. In conclusion, improving conception rate by environmental management increases genetic response indirectly in a breeding program of a closed swine herd. 相似文献
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为了实现机器人玉米秸秆行的精确定位,对耕作玉米机器人的结构进行了改进,并提出了一种基于泰勒级数展开式的RSSI定位方法,提高了机器人玉米秸秆行的定位精度。定位系统使用高清晰度的摄像机采集图像,并采用PID闭环反馈的方式控制机器人的位移,利用PC主控端图像处理,实现了实时定位功能。为了验证机器人玉米秸秆行定位的可靠性,采用田间试验的方法对机器人的性能进行了测试。结果表明:RSSI定位方法的定位精度较高,且图像处理系统可以准确地标定玉米秸秆行,实现机器人在玉米田中的精确定位,避免了机器人在作业过程中对农作物造成损害。 相似文献
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闭式脱粒滚筒的静动态性能分析 总被引:1,自引:0,他引:1
针对联合收割机中闭式脱粒滚筒有可能在外载荷作用下会发生较大变形和在外激励作用下发生共振的问题,采用SolidWorks软件对闭式脱粒滚筒进行了三维建模,以*.x_t格式导入Ansys Workbench建立有限元模型,然后对闭式脱粒滚筒进行静动态性能分析(即静态分析和模态分析)。静态分析结果表明,闭式脱粒滚筒的刚度和强度足够,变形均在设计允许范围之内;模态分析得到的闭式脱粒滚筒前6阶固有频率和振型结果表明,在规定的工况范围内,闭式脱粒滚筒在外激励作用下不会产生共振。研究结果为后续样机的设计和改进提供了理论依据。 相似文献
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Shrimp (Litopenaeus vannamei) production and stable isotope dynamics in clear‐water recirculating aquaculture systems versus biofloc systems 下载免费PDF全文
Closed recirculating aquaculture systems (RAS) offer advantages over traditional culture methods including enhanced biosecurity, the possibility of indoor, inland culture of marine species year‐round and potential marketing opportunities for fresh, never‐frozen seafood. Questions still remain regarding what type of aquaculture system may be best suited for the closed‐system culture of marine shrimp. In this study, shrimp (Litopenaeus vannamei) were grown in clear‐water RAS and in biofloc‐based systems. Comparisons were made between the system types with respect to water quality, shrimp production and stable isotope dynamics used to determine the biofloc contribution to shrimp nutrition. Ammonia and nitrite concentrations were higher, and shrimp survival was lower in the biofloc systems. Although stable isotope levels indicated that biofloc material may have contributed 28% of the carbon and 59% of the nitrogen in shrimp tissues, this did not correspond with improved shrimp production. Overall, the water column microbial communities in biofloc systems may be more difficult to manage than clear‐water RAS which have external filters to control water quality. Biofloc does seem to offer some nutritional contributions, but exactly how to take advantage of that and ensure improved production remains unclear. 相似文献
9.
闽南海区休渔前后张网作业渔获物组成比较分析 总被引:4,自引:1,他引:3
本文主要根据2008年闽南海区张网作业渔业资源监测资料,对休渔前后(4月和8月)张网作业渔获物组成进行比较分析。结果表明:2008年休渔前后都以捕经济种类幼鱼为主。4月带鱼、二长棘鲷、刺鲳、鲐鱼、中华管鞭虾等主要经济种类渔获重量占总渔获物重量69.6%,其中带鱼幼鱼数量最多,占总渔获数量的26.2%(每网渔获尾数744ind),居第一位;且个体极小,基本上全为幼鱼,平均体重8.1g。8月带鱼、二长棘鲷、短尾大眼鲷、叫姑鱼、白姑鱼、须赤虾等主要经济种类渔获重量占总渔获物重量50.9%。带鱼数量明显减少(每网渔获尾数226ind),但个体增大,肛长140mm以上占63.6%,平均体重54.5g,比4月增加46.4g。并结合2004、2007年有关资料,较为详细说明休渔前(4月)张网作业对近岸经济幼鱼、幼体损害比休渔结束后(8月)严重,通过休渔对带鱼等经济种类幼鱼资源起了一定保护作用。 相似文献
10.
控制测量数据内业平差处理时,对于多边形控制网而言,以最小独立闭合环为单位进行平差。基于站点的角度外业观测数据,提出最小独立闭合环的计算机自动快速检索算法。 相似文献