Effects of phenylbutazone and indomethacin on the post-operative course following experimental orthopaedic surgery in dogs

Randomized placebo-controlled crossover studies were carried out in dogs to evaluate how two non-steroidal anti-inflammatory drugs (NSAID) might modulate an acute post-traumatic inflammatory reaction. Two "identical" surgical interventions were performed on the forelimbs of each animal with an interval of 28 days, to enable a paired comparison of the inflammatory signs and the wound/bone healing processes. At one operation 8 dogs received 300 mg phenylbutazone twice daily for 8 days starting on the day before surgery, and at the other operation matching placebo tablets were given. In a similar placebo-controlled trial another group of 8 dogs received 5 mg indomethacin twice daily. With phenylbutazone the post-operative swelling was not significantly reduced compared to placebo, but there was less pain and limping. With indomethacin the swelling was somewhat reduced, but there was no consistent difference to placebo in the pain and limping assessments. None of the drugs appeared to distinctly effect the wound or fracture healing, as evaluated by clinical inspection, comparison of radiographs and comparison of bone sections from the sites of surgery. It proved difficult to select an appropriate dosage of indomethacin due to its high potential to induce GI ulceration and bleeding in dogs. In this experimental surgical model with an acute inflammation, neither phenylbutazone nor indomethacin showed impressive anti-inflammatory or analgesic properties. In the same model paracetamol has proved to significantly and more efficiently, reduce both swelling and pain without any noticeable adverse effects, and appears to be a better alternative than the two presently tested NSAID.     

不同药剂对苹果树腐烂病伤口愈合效果研究

为探讨苹果腐烂病的有效防治方法,研究比较了春季休眠期和萌芽期用纯泥、多菌灵药泥、拂蓝克、人工树皮二代和愈合剂5种药剂处理对苹果树伤口愈合面积和形成愈伤组织鲜干重的影响。结果表明:休眠期形成的伤口愈合情况好于萌芽期形成的伤口;使用多菌灵药泥包裹处理伤口愈合面积、愈合比例最大,分别为7.05cm2和99.76%。愈伤组织鲜干重也最大,伤口愈合效果最好。建议春季在休眠期使用多菌灵药泥对苹果腐烂病进行伤口处理。     

重组人骨形态发生蛋白2复合物对犬胫骨骨折愈合中4种相关细胞因子的作用

研究重组人骨形态发生蛋白2(rhBMP-2)、碱性成纤维细胞生长因子(bFGF)、纤维蛋白凝胶(FG)与妥布霉素复合物加速骨折愈合。bFGF与rhBMP-2和妥布霉素以纤维蛋白胶为载体复合,将具有抗生素缓释系统的复合物注入犬胫骨骨折处,并做内固定,于术后第2周,采集犬胫骨骨折处样品,每隔2周采集1次,共采集4次,将每次采集的样品制成组织切片,采用免疫组织化学的方法检测4种因子,并对组织切片进行分析,用多功能真彩色细胞图象分析管理系统,分析所得数据用SPSS18.0版统计软件进行统计学分析。血管内皮细胞生长因子(VEGF)在试验组第2周表达强阳性,而对照组阳性表达较弱;前4周,试验组血小板源性生长因子(PDGF)阳性率呈上升趋势,强于对照组;前8周,试验组胰岛素样生长因子(IGF)和转化生长因子-β1(TGF-β1)阳性率均高于对照组。说明bFGF与rhBMP-2和妥布霉素以纤维蛋白胶为载体的复合物在骨折愈合具有中促进细胞增殖、黏附、趋化、分化,以及骨折末端血管生长和形成的作用。     

Marine Collagen: A Promising Biomaterial for Wound Healing,Skin Anti-Aging,and Bone Regeneration

Marine organisms harbor numerous bioactive substances that can be utilized in the pharmaceutical and cosmetic industries. Scientific research on various applications of collagen extracted from these organisms has become increasingly prevalent. Marine collagen can be used as a biomaterial because it is water soluble, metabolically compatible, and highly accessible. Upon review of the literature, it is evident that marine collagen is a versatile compound capable of healing skin injuries of varying severity, as well as delaying the natural human aging process. From in vitro to in vivo experiments, collagen has demonstrated its ability to invoke keratinocyte and fibroblast migration as well as vascularization of the skin. Additionally, marine collagen and derivatives have proven beneficial and useful for both osteoporosis and osteoarthritis prevention and treatment. Other bone-related diseases may also be targeted by collagen, as it is capable of increasing bone mineral density, mineral deposition, and importantly, osteoblast maturation and proliferation. In this review, we demonstrate the advantages of marine collagen over land animal sources and the biomedical applications of marine collagen related to bone and skin damage. Finally, some limitations of marine collagen are briefly discussed.     

番茄与黄瓜远缘嫁接愈合口形成过程的组织解剖分析

为探明番茄/黄瓜远缘嫁接不亲和时愈合口的形成过程,为后期砧穗间信息互作的研究奠定一定的理论基础,以黄瓜‘新泰密刺’、黑籽南瓜和番茄‘中杂9号’为试材,采用下胚轴斜靠接方法远缘嫁接番茄/黄瓜(接穗/砧木),嫁接组合黄瓜/南瓜、黄瓜/黄瓜作为嫁接亲和性的对照。观察嫁接后第2、5、8和15天嫁接苗生长形态变化并统计成活率,对比分析黄瓜的近缘及远缘嫁接的嫁接口愈合过程。结果表明:1)嫁接接口处石蜡切片结果显示,对照黄瓜自嫁接和黄瓜与南瓜的嫁接均经历了相同的愈合过程,并最终成功地建立了形成层与输导组织,得以正常生长;2)分别用经木质部运输的品红溶液、经韧皮部运输的HPTS染料标记嫁接苗,结果显示远缘的番茄与黄瓜嫁接后,砧木与接穗虽然可以各自分化出愈伤组织,但二者愈伤组织的薄壁细胞无法真正地融合,机械性地对接在一起的木质部可以运输水分,使得番茄接穗不萎蔫,但韧皮部并未连通,无法进行其光合及同化产物运输、维持其生命活动,初步解释了远缘嫁接不亲和的解剖学过程。     

An Electrospun Scaffold Loaded with an Enteromorpha Polysaccharide for Accelerated Wound Healing in Diabetic Mice

The design and development of innovative multifunctional wound dressing materials in engineered biomaterials is essential for promoting tissue repair. In this study, nanofibrous wound dressing materials loaded with anti-inflammatory ingredients were manufactured by a promising electrospinning strategy, and their capability for treating diabetic wounds was also investigated. A scaffold blend consisting of an Enteromorpha polysaccharide and polyvinyl alcohol (PVA) was fabricated. The in vitro and in vivo studies confirmed the efficacy of PVA/EPP1 fiber. We found that PVA/EPP1 fiber accelerated the repair of a full-thickness skin wound in diabetic mice. The results suggest that this scaffold could effectively shorten the wound healing time by inhibiting inflammatory activity, which makes it a promising candidate for the treatment of hard-to-heal wounds caused by diabetes.