牛皮肤成纤维细胞的体外培养与冻存

利用牛皮肤组织块直接培养法，得到牛皮肤细胞的原代培养物，再用酶消化法和反复贴壁法处理，能够纯化成纤维细胞。成纤维细胞的冻存是通过选用6种分别含有二甲基亚砜（DMSO）、甘油（GL）及乙二醇（EG）的保护液，以相同的冻前处理方法，对牛皮肤成纤维细胞进行缓慢冷冻，冰箱预冷平衡1－2h，逐步投入液氮（－196℃）中保存，再经37℃水浴解冻，Hanks液脱保护剂，以贴壁率评价冻存效果。结果表明，20％DMSO保护液对牛皮肤成纤维细胞表现出较好且稳定的冷冻保护效果，其平均贴壁率达87．9％。     

Suppurative Inflammation of Apocrine Sweat Glands (Suppurative Hidradenitis) in the Dog: A Retrospective Clinicopathological Analysis of 100 Cases

Résumé— Une analyse rétrospective clinicopathologique a été menée chez 100 chiens présentant une hydradénite suppurative diagnostiquée par biopsie cutanée. Aucune prédisposition d'âge, de race ou de sexe n'a été mise en évidence. Tous les chiens présentaient cliniquement une folliculite bactérienne, ou une furonculose, ou les deux, qu'elle soit primaire ou secondaire à d'autres dermatoses. Aucene lésion clinique n'était pathognomonique d'hidradénite suppurative. Chez 73 chiens, l'hidradénite suppurative était associée microscopiquement à des degrés variables d'inflammation du follicule pileux. Chez 27 chiens, l'hidradénite suppurative était la seule évidence histologique d'inflammation annexielle. L'observation histopathologique d'hidradénite suppurative suggère l'existence sur le plan clinique d'une folliculite bactérienne, d'une furonculose, ou des 2. [Scott, D.W. Suppurative inflammation of apocrine sweat glands (suppurative hidradentis) in the dog: a retrospective clinicopathological analysis of 100 cases (inflammation suppurative des glandes sudorales apocrines (hidradénite suppurative) chez le chien: une analyse rétrospective clinicopathologique de 100 cas). Resumen— Se llevó a cabo un estudio clinicopatológico retrospectivo en 100 perros con hidradenitis supurativa diagnosticada mediante biopsia cutánea. No se detectó predominancia alguna de raza, sexo o edad. A todes los perros se les habia diagnosticado clinicamente foliculitis o furunculosis bacteriana, o ambas, ya sea de forma primaria o secundaria a otras dermatosis. No se encontraron lesiones clinicas especificas indicativas de hidradenitis supurativa. En 73 animales, la hidradenitis supurativa estaba asociada con varios grados de inflamación folicular a nivel microscópico. En los 27 casos restantes, los anejos presentaban unicamente hidradenitis supurativa. La presencia de hidradenitis supurativa a nivel microscópico sugiere la existencia de foliculitis o furunculosis bacteriana clinicas, o de ambas. [Scott, D.W. Suppurative inflammation of apocrine sweat glands (suppurative hidradenitis) in the dog: a retrospective clinicopathological analysis of 100 cases (inflamación supurativa de las glándulas sudoriparas apocrinas (hidradenitis supurativa) en el perro: estudio clinicopatológico retrospectivo de 100 casos). Abstract— A retrospective clinicopathological study was conducted on 100 dogs with suppurative hidradenitis as determined by skin biopsy. No apparent age, breed, or sex predilections were recognized. All dogs had been given a clinical diagnosis of bacterial folliculitis, furunculosis, or both of these, whether primary or secondary to other dermatoses. There were no clinical lesions that uniquely suggested the presence of suppurative hidradenitis. In 73 dogs, suppurative hidradenitis occurred in conjunction with varying degrees of hair follicle inflammation microscopically. In 27 dogs, suppurative hidradenitis was the only histological evidence of adnexal inflammation. The histopathologic finding of suppurative hidradenitis suggests the existence of clinical bacterial folliculitis, furunculosis, or both of these.     

Differential effects of -mannose and 2-deoxy- -glucose on attempted powdery mildew fungal infection of inappropriate and appropriate ramineae

Barley, oat and wheat were used as both inappropriate hosts (IH) and appropriate hosts (AH) for three formae speciales of the fungus Blumeria graminis, the causal agent of powdery mildew disease. Treatment with either the glucose analog 2-deoxy- -glucose (DDG) or with -mannose dramatically suppressed penetration resistance in IH and to a much lesser extent in AH combinations. Other effects of DDG and -mannose were strikingly dissimilar. DDG greatly reduced localized autofluorescence at fungal attack sites on epidermal cells, and prevented hypersensitive epidermal cell death (HR). -mannose had little effect on autofluorescence or HR. DDG arrested the development of fungal haustoria and apparently prohibited biotrophy leading to secondary hyphae. -mannose allowed haustorial development and functional biotrophy leading to the production of elongating secondary hyphae. This suggests that B. graminis is in some way capable of utilizing -mannose as a carbon substrate. Results with IH combinations paralleled those of known mlo -barley responses to DDG and -mannose. Results are discussed in relation to specific physiological processes known to be influenced by either DDG or by -mannose, or by both compounds.     

遮穗和去颖下燕麦穗部特征变化和穗部光合贡献率估算

随着探索提高植物整体光合能力相关研究的不断开展，麦类作物穗部器官等植物非叶绿色器官光合潜力挖掘逐渐得到关注。本研究在成都平原秋播美达、贝勒、莫尼卡、摄政王、泰克和甜燕60等6个品种燕麦，设置遮穗、去颖2个试验处理，比较分析了各品种间穗部特征、穗部光合贡献率、颖片光合贡献率和茎光合物质转移率等差异。结果表明，燕麦穗部器官光合贡献率为28.56%～49.05%，其中甜燕60最高；6个品种燕麦的颖片光合贡献率为11.03%～36.88%，茎光合物质转移率为6.65%～35.81%。燕麦穗部器官对籽实增重表现了较高的光合贡献，当燕麦穗部器官光合受到限制时，燕麦单粒种子重和单穗种子数显著降低，尤其是影响双粒小穗数。     

景泰绿洲3种小谷物的产草量与牧草营养成分预测

为了预测景泰绿洲春小麦(Triticum aestivum)、燕麦(Avena sativa)、黑麦(Secale cereale)的产草量和营养品质,通过田间试验,测得牧产草量、株高和分蘖,室内测定粗蛋白、粗脂肪、中性洗涤纤维、酸性洗涤纤维、粗灰分和可溶性碳水化合物,分析各指标之间的相关性。结果表明,收获干草的3种小谷物分蘖和株高均与可溶性碳水化合物之外其它5个营养指标存在显著相关性(P0.05),模拟轮牧的春小麦和黑麦株高与营养指标均无相关性(P0.05),燕麦和黑麦的分蘖与6个营养指标均呈显著相关(P0.05)。建立了根据株高预测产草量和营养品质的模型、用分蘖预测牧草营养品质的模型、用产草量预测牧草营养品质的模型、用分蘖和产草量两个因子综合预测营养品质的模型。经过与实测值对比,各预测模型的准确性均较高。     

不同燕麦品种在尼泊尔北部山区的生长特性及其营养品质的研究

畜牧业是尼泊尔北部山区的重要生计支柱,提升牧草产量是我国对尼泊尔开展农业技术援助的关键领域。为筛选出适宜尼泊尔北部山区栽培的燕麦品种,于2019年5-10月在尼泊尔热索瓦县郎唐山区对12个燕麦品种（爱沃、太阳神、贝勒1、美达、科纳、林纳、青引1号、青海444、青海甜燕麦、陇燕2号、陇燕3号、Kamadhenu）的物候期、株高、产草量、穗含量、叶茎比和关键营养成分等进行了品比试验。结果表明：美达、科纳、青引1号、青海444、青海甜燕麦和Kamadhenu 6个品种能完成生育期,生育天数在115~141 d,其余多数品种只能达到乳熟期。各燕麦品种的株高为134.8~177.7 cm,其中引进品种太阳神、青海444、青海甜燕麦、美达和林纳的株高较对照品种Kamadhenu高6.3%~20.4%,存在极显著差异（P<0.01）。青海甜燕麦、青海444和美达的干草产量分别达到了14723.0、13491.0和13369.6 kg·hm^-2,分别比对照品种Kamadhenu增产36.0%、24.7%和23.6%,差异极显著（P<0.01）。贝勒1、科纳和太阳神的叶茎比分别为0.40、0.38和0.36,是对照品种Kamadhenu的1.50~1.67倍。各燕麦品种的干物质、粗蛋白、总灰分、中性洗涤纤维和酸性洗涤纤维含量变化范围分别为93.5%~95.6%、5.7%~9.9%、4.4%~6.9%、68.2%~78.4%和39.3%~48.7%,其中太阳神的粗蛋白含量是对照品种Kamadhenu的1.57倍,存在极显著差异（P<0.01）。对各燕麦品种的10个农艺性状进行主成分分析及综合评价,结果表明,引进品种青海甜燕麦、美达、青海444和太阳神综合适应性较好,适宜在该地区推广种植。     

Photodynamic therapy of feline cutaneous squamous cell carcinoma using a newly developed liposomal photosensitizer: preliminary results concerning drug safety and efficacy

BACKGROUND: Squamous cell carcinomas are common skin tumors in cats. We investigated photodynamic therapy (PDT) using a new liposomal photosensitizer as a minimally invasive, effective treatment that can be easily performed while achieving good cosmetic results. AIM: The goal of this study was to assess and describe possible toxicities using a liposomal formulation of the photosensitizer meta-(Tetrahydroxyphenyl)Chlorin (m-THPC) and investigate if favorable pharmacokinetics translate into favorable tumor response and control. ANIMALS: Eighteen client-owned cats with 20 spontaneous cutaneous squamous cell carcinomas were included in the study. METHODS: PDT was performed using a new, liposomal formulation of the photosensitizer. Toxicity, tumor response, and tumor control were evaluated retrospectively. RESULTS: No general adverse effects were observed in cats treated with the new liposomal formulation. Mild local toxicity such as erythema and edema were seen in 15% of the patients. All cats responded to therapy, with a complete response rate of 100%. The overall 1-year control rate was 75%. The tumor recurrence rate was 20% with a median time to recurrence of 172.25 +/-87.1) days. CONCLUSIONS AND CLINICAL IMPORTANCE: A new liposomal photosensitizer was successfully used for squamous cell carcinoma in cats and was well tolerated. There were no systemic adverse effects observed with the liposomal formulation. The favorable pharmacokinetics of the liposomal drug resulted in a favorable tumor response.     

节节麦与野燕麦8倍体杂种核型分析

节节麦(Aegilops tauschii(Coss.)Sch.2n=2x=14)与通北野燕麦(Avena fatua L.2n=6x=42)杂交,F_1自然加倍成8倍体,8倍体播成的F_2代,遗传性基本一致,PMC镜检表明,染色体数为2n=28Ⅱ。用F_2所结的种子进行根尖细胞核型分析,结果发现8倍体中包含有全套的节节麦和野燕麦的染色体。节节麦中有1对随体染色体.2对近中部着丝点染色体,4对中部着丝点染色体。野燕麦有3对随体染色体,5对近端着丝点染色体,8对近中部着丝点染色体,5对中部着丝点染色体。而节节麦与野燕麦8倍体杂种,有4对随体染色体,5对近端着丝点染色体.10对近中部着丝点染色体,9对中部着丝点染色体。核型分析结果表明,节节麦与野燕麦杂交合成的8倍体,是节节麦与野燕麦的双二倍体。