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丝瓜络化学成分分析 总被引:8,自引:0,他引:8
采用系统预试法,对丝瓜络的水和乙醇浸提物进行化学成分定性检验,石油醚浸提物进行GC-MS分析,并采用电感耦合等高子体发射光谱仪(ICP-AES)对丝瓜络中13种微量元素进行定量分析.初步推断丝瓜络中含有蛋白质、氨基酸、多肽、多糖、甙类(皂甙,强心武)、有机酸、蒽醌类、酚类、鞣质、黄酮、香豆素、萜内酯及生物碱;可能含有甾体、三萜类化合物;不含挥发油、油脂.丝瓜络石油醚提取物经CC-MS分析,共鉴定出32种化合物,主要为酸、酯、烷类化合物,其中酸类11种,酯类7种,烷类9种,分别占检出化合物总含量的52.27%、13.34%和5.23%.各成分中相对含量最高的是棕榈酸(28.33%),其次依次为十八烷酸(14.98%),二乙二醇硬脂酸酯(7.87%),顺式-9-十六烯醛(7.56%),十八烷酸丙酯(3.48%).丝瓜络中人体所必需的钙、镁、磷、钾、铁元素含量相当高,而铅、镉等有害的重金属元素含量却很低.其含量顺序为:钙>镁>磷>钾>铁>铝>钠>锌>锰>铜>镍>铅>镉. 相似文献
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SIQIN Mari NAKAI Tomohiro HAGI Shinichi KATO Ali Mohammed PITIA Mai KOTANI Yuki ODANAKA Yasushi SUGAWARA Koh‐Ichi HAMANO Keiichiro YOGO Yoshio NAGURA Masaru FUJITA Hiroshi SASADA Eimei SATO Tetsuya KOHSAKA 《Animal Science Journal》2010,81(6):681-686
Relaxin‐like factor (RLF), also known as insulin‐like factor 3 (INSL3), is produced by testicular Leydig cells, but its specific receptor LGR8 (leucine‐rich repeat family of G‐protein‐coupled receptor 8) has not been identified in goats. This study aimed to identify complementary DNA (cDNA) sequences of goat LGR8, and characterize the expression of both RLF and LGR8 in goat testes by RT‐PCR and immunohistochemistry. Testes were collected from immature (3‐month‐old) and mature (24‐month‐old) Saanen goats, and partial cDNA sequences of the goat homologue of human LGR8 were identified. The sequence encoded a reduced peptide sequence of 167 amino acids, which corresponded to transmembrane regions 2 through 5, followed by the beginning of intracellular loop 3 of human LGR8. Expression of both LGR8 and RLF genes was drastically increased in mature testes compared with immature ones. Although RLF protein was restricted to Leydig cells, LGR8 protein was detected in both Leydig cells and seminiferous epithelial cells (possibly germ cells and Sertoli cells). These results reveal a possible existence of the RLF‐LGR8 ligand‐receptor system within the goat testis, suggesting that RLF may play a role in testicular function through LGR8 on Leydig cells and seminiferous epithelial cells in an autocrine and/or paracrine manner. 相似文献
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