猪卵巢发育的组织学观察及磷酸二酯酶3A的表达研究

为研究母猪卵巢在不同发育阶段的组织学变化规律和探讨磷酸二酯酶3A(PDE3A)在卵巢发生发育和成熟过程中的生物学意义,选用二花脸母猪胎90日龄及1日龄、20日龄、120日龄和180日龄的母猪卵巢进行组织学观察,并利用RT-PCR检测PDE3A mRNA的表达情况。结果显示:胎90日龄主要由原始卵泡组成;1日龄、20日龄、120日龄卵巢内特征发育明显;180日龄卵巢内的形态特点与120日龄相似。卵巢PDE3A mRNA表达水平在20日龄时与其他各个阶段相比显著降低。提示:PDE3A在猪从原始卵泡募集到初级卵泡形成过程中可能具有生物学作用。     

自适应耦合全变分和四阶PDE的图像放大

目的 提出一种新的基于偏微分方程(PDE)的图像放大算法.方法 算法模型由全变分、四阶PDE和数据保真项构成,将图像演化的过程转为PDE模型,进而通过迭代求解方程得到放大图像.结果 通过设置与图像局部特征有关的连续函数作为调节全变分和四阶PDE的权重,实现两种PDE的自适应耦合,避免了全变分模型在放大时出现分块效应;通...     

PDE3与亮甲酚蓝在绵羊卵母细胞体外培养中的应用研究

[目的]探讨磷酸二酯酶3（PDE3）特异性抑制剂Milrinone与亮甲酚蓝染色液（BCB）相结合在绵羊卵母细胞体外培养中的应用。[方法]比较了BCB对绵羊卵母细胞的筛选与传统的形态分级之间的差异,以及对胚胎后期发育的影响;研究了Milrinone对绵羊卵母细胞的最佳抑制时间,并用于BCB-卵母细胞体外培养。[结果]A、B级卵母细胞中BCB＋卵母细胞的比例为64.42%,极显著高于C级卵母细胞的17.00%;BCB＋卵母细胞的成熟率（86.16%）、卵裂率（85.29%）、囊胚率（34.40%）分别极显著高于BCB-卵母细胞（50.94%、36.19%和6.73%）;6 h是Milrinone对绵羊卵母细胞的最佳抑制时间,该试验组体外培养效率显著高于其他组;Milrinone对BCB-卵母细胞抑制培养6 h,极显著提高了体外胚胎发育率。[结论]为提高绵羊卵母细胞体外培养效率提供了依据。     

重调和方程Zienkiewicz元逼近的多重网格法

本文研究重调和方程Zienkiewicz元逼近的多重网格法,证明了h无美收敛性,并得到了多重网格套迭代解与边值问题真解的最优阶误差估计.     

改进BSCB与样本块耦合的单板节子缺陷图像修补算法

分析了我国旋切单板生产过程中对单板节子缺陷挖补上存在的问题,提出了将图像修复理论、方法应用于单板节子表面缺陷图像的自动修补中,以提高旋切单板生产过程的自动挖孔补节智能化水平。针对BSCB算法在单板节子表面缺陷图像修复上存在的不足,提出了一种新的BSCB改进算法,使其在单板节子图像结构修复上得到比较好的效果。但是该算法对单板节子区域较大、节子周围纹理比较多的情况,修复效果不是很好。为此,又提出了将改进后的BSCB算法与基于样本块的图像修复算法相耦合的单板节子缺陷图像修复新方法,实现了对旋切单板节子缺陷图像的结构和纹理部分的修复,并通过数值实验验证了该方法的有效性。     

基于四阶偏微分方程的乘性噪声去除模型

基于二阶偏微分方程(PDE)在复原图像时会产生阶梯现象, 而高阶PDE能够很好地克服这一缺陷, 提出了一个针对乘性噪声去除的四阶PDE模型. 为了求解该模型, 建立了一个交替最小值算法, 并通过数值试验验证了该算法的有效性.     

Nature’s Lab for Derivatization: New and Revised Structures of a Variety of Streptophenazines Produced by a Sponge-Derived Streptomyces Strain

Eight streptophenazines (A–H) have been identified so far as products of Streptomyces strain HB202, which was isolated from the sponge Halichondria panicea from the Baltic Sea. The variation of bioactivities based on small structural changes initiated further studies on new derivatives. Three new streptophenazines (I–K) were identified after fermentation in the present study. In addition, revised molecular structures of streptophenazines C, D, F and H are proposed. Streptophenazines G and K exhibited moderate antibacterial activity against the facultative pathogenic bacterium Staphylococcus epidermidis and against Bacillus subtilis. All tested compounds (streptophenazines G, I–K) also showed moderate activities against PDE 4B.     

Associations of Two Polymorphic Loci: A HinfⅠLocus of the Porcine Subunit C of Succinate Dehydrogenase Complex (SDHC) Gene and A MspⅠLocus of the Porcine Rod cGMP-Phosphodiesterase γ-Subunit (PDE6G) Gene

A Hinf I locus of the porcine subunit C of succinate dehydrogenase complex (SDHC) gene and a Msp I locus of the porcine rod cGMP-phosphodiesterase γ-subunit (PDE6G) gene had been reported before, but the association analysis between the different genotypes and the traits had not been done. 300 Large White × Meishan F2 pigs were used as experimental materials to performe the PCR-RFLP analysis and association analysis for the two loci, results revealed that the polymorphism of the porcine subunit C of succinate dehydrogenase complex (SDHC) gene was significantly associated with the traits which included the carcass length, the estimated lean meat percentage, the estimated backfat thickness at last rib, the estimated backfat thickness at last 3-4th rib, the fat meat weight, the fat meat percentage, the lean meat weight,the lean meat percentage, the ratio of lean meat to fat meat, the leaf fat weight, the backfat thickness at shoulder, the backfat thickness at thorax-Waist, the backfat thickness at 6-7th thorax and the average daily gain. Seven other traits, the meat color value (Biceps femoris, BF), the meat marbling (Biceps femoris, BF), the water moisture (Longissimus dorsi, LD),the bone weight, the bone percentage, the loin eye width and the loin eye area, were found to be significantly correlated with the polymorphism of the porcine rod cGMP-phosphodiesterase γ-subunit (PDE6G) gene. Based on these results, it is necessary to apply the two genes as candidate genes to marker assistant selection (MAS) in pig breeding.     

低温法制备马铃薯淀粉磷酸双酯的工艺研究

摘要：试验在40℃的低温条件下,以三氯氧磷为交联剂,聚乙二醇（PEG）为催化剂制备马铃薯淀粉磷酸双酯。在单因素试验基础上,选取PEG、三氯氧磷和pH三个因素做正交试验,以优化制备工艺。结果表明：当稳定剂用量为0.1%、三氯氧磷用量为0.05%、pH为10时所制备的马铃薯淀粉磷酸双酯的粘度为3400cp,酯化度为0.42。     

VPK合成、活性及吸收转运特性研究

候选新药VPK是哈药集团技术中心自主研发治疗脑卒中的一类新药。VPK以长春西汀为母核,经水解、缩合、环化、成盐反应制备而成,经元素分析、核磁共振、高分辨质谱、X-射线粉末衍生,X-射线单晶衍射对化合物进行结构确证,经PDE1活性研究显示,VPK和长春西汀均对PDE1有抑制作用,其IC50值分别为17.40和1.97μmol·L~(-1),VPK在脑卒中治疗领域疗效较好,采用Caco-2单层细胞模型测定候选药物VPK吸收及外排转运特性,结果表明,VPK在顶端到基底端(A-B)及基底端到顶端(B-A)方向均表现高渗透,该供试化合物是外排转运体底物可能性较小。