产后奶牛子宫内膜炎神经肽（SP、VIP）对子宫免疫调节的研究

本试验研究了急性产后子宫内膜炎神经肽（SP、VIP）变化,以探讨子宫内膜炎神经肽对子宫局部免疫的影响。对10头产后急性化脓性子宫内膜炎患牛进行子宫内膜及血清SP、VIP含量的检测,并选择10头产）06-8d健康的奶牛为对照组。结果表明,产后急性子宫内膜炎患牛子宫内膜及血清中SP含量显著升高,试验组与对照组差异极显著（P〈0．01）,而VIP含量显著降低,试验组与对照组差异极显著（P〈0．01）。急性产后子宫内膜炎属于局部炎症,其发生与神经肽（SP、VIP）浓度密切相关,sP含量的升高和VIP含量的降低对机体的免疫起调节作用。     

Role of mast cells in pain of adjuvant arthritis in mice

AIM To investigate the role of mast cells in the pain of adjuvant arthritis (AA) in mice induced by complete Freund's adjuvant (CFA). METHODS The animals were divided into 4 groups: normal control group (control group), AA model group (model group) and AA model + cromolyn sodium (CS) group (CS group), AA model + mast cell lacking group (W-4Bao group), with 6 mice in each group. The animals in the first 3 groups were C57BL/6 mice, while those in W-4Bao group were Kit^W-4Bao mice lacking of mast cells. The pain model of chronic AA was induced by intraplantar injection of CFA into the right hind paws of the mice, while the mice in control group was injected with saline. One day after CFA injection, the mice in CS group were intraperitoneally injected with CS (20 mg/kg), and those in other groups received an equal volume of saline once a day for 14 d. The paw edema, paw withdrawal threshold (PWT) and paw withdrawal latency (PWL) were evaluated at 0, 1, 3, 7, 10 and 14 d after CFA injection. The mouse right ankle joint was harvested after 14 d for HE and toluidine blue staining, and the concentrations of histamine, tryptase, substance P (SP), calcitonin gene-related peptide (CGRP) were detected by ELISA. RESULTS One day after CFA injection, the inflammation of the right hind paw in model group was more serious compared with control group, and the PWT and PWL were notably decreased (P<0.05). In addition, the numbers of mast cells and degranulated mast cells were increased obviously, and the concentrations of histamine, tryptase, SP and CGRP were increased (P<0.05). However, compared with model group, hyperalgia and the release of neuropeptides in CS group and W-4Bao group were significantly decreased (P<0.05). CONCLUSION The activation of mast cells promotes the pain of AA in mice, and its mechanism may be associated with the release of neuropeptides and relevant inflammatory factors.     

Effects of hypothermia on the contents of neuropetides and other active substances in rat plasma and different brain regions

AIM: To study the effects of hypothermia on the contents of AVP,Ang Ⅱ,L-EK,cAMP,Mg²⁺and Ca²⁺in rat plasma and different brain regions.METHODS: The model of experimental hypothermia rat was established and treated in groups randomly.At the end of the experiment,samples of plasma and braintissues were taken and the contents of L-EK,cAMP,AVP,Ang Ⅱ were determined by RIA methods,Mg²⁺and Ca²⁺by atom absorption methods.RESULTS: The contents of Ang Ⅱ,AVP increased significantly in the plasma of Ⅰ,Ⅲ group(P<0.01),while that of group Ⅱ decreased obviously (P<0.01);the contents of Ang Ⅱ,AVP decreased in the hypothalamus and truncus encephalicus of Ⅰ,Ⅲ group(P<0.01),and that of group Ⅱ increased (P<0.01).CONCLUSION: L-EK,cAMP,AVP,Ang Ⅱ,Mg²⁺and Ca²⁺might be involved in body temperature regulation during hypothermia.