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HUANG Yong-hong SHI Hui-mian ZHOU Xiao-yan XU Hong LUO Zhi-jun XU Fang-yun 《园艺学报》2015,31(7):1340-1344
Receptor type protein tyrosine phosphatase Q (PTPRQ) is an unusual protein tyrosine phosphatase that has intrinsic dephosphorylating activity for various phosphatidylinositiol and phospho-tyrosine substrates, especially the phosphatidylinositol activity. Recent data show that PTPRQ has an important role in various biological processes and is associated with some diseases. In this article, the structure and function of PTPRQ and the relationship between PTPRQ and diseases were briefly summarized. 相似文献
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LI Qing-fen LI Zhuo-ya GONG Fei-li XU Yong JIANG Xiao-dan FENG Wei XIONG Ping 《园艺学报》2001,17(8):710-713
AIM: To compare the tumorigenecity of H22 cells transfected with TNF-α gene and its mutants(secreted TNF-α mutant, S-TNFm, transmembrane TNF-α mutant, TM-TNFm and wild type of TNF-α, Wt-TNF) in vivo. METHODS: Three kinds of mouse liver cancer cell line H22 expressing TNF-α and its two mutants were mixed with untransfected H22 at different effector/target ratio separately. The growth of tumor was examined after injection of 2.5×105(100 μL) mixed H22 tumor cells into mice. The lymphocyte infiltration in the site of tumor and the expression of Fas on tumor cells were detected by immunohistochemistry. RESULTS: The tumorigenecity of H22 cells transfected with TNF-α gene and its mutants was significantly weakened( P <0.01). Besides the cytotoxicity of the both forms of TNF, TM-TNF was found to induce the expression of death receptor Fas by tumor cells and S-TNF was shown to promote frank lymphocyte infiltration in the site of tumor. Furthermore, a transient decrease in body weight was found in mice inoculated with H22/S-TNFm. CONCLUSION: The tumorigenecity of tumor cells was reduced by transfection with TM-TNF or S-TNF gene. It results from the cytotoxicity of TNF and their activation of tumorcidal mechanisms in vivo. TM-TNF may induce tumor cell apoptosis via the Fas pathway while S-TNF may exert its antitumor effect by recruiting and activating lymphocytes in the site of tumor. 相似文献
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An investigation was undertaken to study two different types of farrowing pens, use being made of well characterized veterinary parameters for the well‐being of piglets. A housing system with a solid floor and straw‐bedding was compared with a partly metal‐slatted floor system. The veterinary parameters we used were mortality, morbidity, and external injuries in piglets. There were slight differences in mortality and morbidity rates between the two housing systems. There were big differences with regard tp the piglets” injury‐index’ of the two housing systems. The straw system was more favourable. The studies show that obervations of external injuries ('Method of Ekesbo') can be used to judge housing systems of piglets in the farrowing house. 相似文献
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P. van der Meer G. Uilenberg S. G. van den Bergh A. A. M. Spanjer N. M. Perié 《The Veterinary quarterly》2013,33(2):61-65
Summary Bovine blood containing piroplasms of Theileria parva, as well as non‐injected blood, was lysed and subjected to iso‐electric focussing. Staining for 13 different enzymes revealed parasite‐associated bands of glucose phosphate isomerase (GPI) activity, not of any of the other enzymes. There were no variations between individual donor animals in the host cell GPI bands and these bands did not interfere with the recognition of the parasite‐associated bands, so that purification of the piroplasms was unnecessary. Blood from cattle infected with T. mutans also gave parasite‐associated bands of GPI, but no such bands were seen in zymograms of blood from cattle infected with a Theileria sp. from Japan. Depending on the level of parasitaemia, up to four parasite‐associated bands were found in one strain of T. parva and up to three in two other strains. Among the disadvantages of using piroplasm material for the study of isoenzymes of T. parva is the fact that animals often die before their parasitaemia is sufficiently high, and that some strains never give rise to a high parasitaemia. 相似文献
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Many snake venoms contain complex mixtures of pharmacologically important molecules, some of which show potential therapeutic value in the treatment of cancer and other human disorders. In this review, we mainly reports the effects of snake venom active components, such as disintegrins and lectins in paralyzing cancer cells, blocking on cell migration, interaction with integrins, inhibition of tumor dissemination and angiogenesis. The advanced researches on the snake venom's apoptosis-inducing components on tumors are also introduced. 相似文献
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Endostatin, a 20 kD (184 aa) C-teminal fragment of collagenⅩⅧ, is the most potent inhibitor of tumor angiogenesis described so for. Endostatin was initially isolated from a murine hemangioendothelioma cell line (EOMA). Purified recombinant murine endostatin generated in E. coli bacteria injected as unfolded suspension, inhibited the growth of a varity of metastatic and primary tumors in mice. However, its widespread application has been hampered by difficulties in the large-scale production of the antiangiogenic proteins. The limitation may be resolved by in vivo delivery and expression of the antitangiogenic gene. This review summarized the advances in endostain research in recent years including structure, the mechanism of generating, function and therapy. 相似文献
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HUANG Kai-hong LIU Jian-hua WANG Ling-yun ZHU Zhao-hua CHEN Qi-kui MIN Jun CHEN Ru-fu 《园艺学报》2007,23(9):1706-1709
AIM: To investigate the preparation techniques and anti-tumor effects both in vitro and in vivo of a novel nanoparticles control-releasing preparation of 5-fluorouracil (5-FU) by intravenous injection.METHODS: With polylactic acid (PLA) as marix materials,we adopted ultrasound emulsification method to prepare PLA enveloped 5-FU nanoparticles (5-FU-NPs).Scanning electricity microscopy was used to observe the morphology of 5-FU-NPs and laser optical scattering experiment was conducted to determine its diameter distribution.The drug-carrying capacity (ratio) of the nanoparticles was determined by means of high-power liquid chromatography(HPLC) and MTT test was used to observe cytotoxicity in vitro.The anti-tumor effects were determined at different dosages,frequencies of taking drugs in vivo.RESULTS: Scanning electron microscopy showed that the 5-FU-NPs were globular particles with smooth surface in an average particle diameter of 191.9 nm with a normal distribution,and the drug-carrying capacity of 5-FU-NPs was 15.2%.5-FU-NPs had the same anti-cancer effect as unenveloped drug in vitro and showed typical dose-effect relationship.Compared to naked 5-FU,5-FU-NPs presented significant difference (P<0.05) in anti-cancer effect.CONCLUSION: Nanoparticles may serve as effective carrier for controlled release of 5-FU,which lead to reasonable administration of 5-FU with less toxicity. 相似文献
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Anette B Grossi J?rgen S Agerholm Knud Christensen Henrik E Jensen Páll S Leifsson Christian Bendixen Peter Karlskov-Mortensen Merete Fredholm 《Acta veterinaria Scandinavica》2014,56(1)