Tuberculosis vaccination sequence effect on protection in wild boar

The Eurasian wild boar (Sus scrofa) is a reservoir for tuberculosis (TB) in which vaccination is a valuable tool for control. We evaluated the protection and immune response achieved by homologous and heterologous regimes administering BCG and heat-inactivated Mycobacterium bovis (IV). Twenty-one wild boar piglets were randomly allocated in five groups: Control, homologous BCG, homologous IV, heterologous IV-BCG, heterologous BCG-IV. Significant 67% and 66% total lesion score reductions were detected in homologous IV (IVx2) and heterologous IV-BCG groups when compared with Control group (F_4,16 = 6.393, p = 0.003; Bonferroni _{Control vs IVx2} p = 0.026, Tukey _{Control vs IV-BCG} p = 0.021). No significant differences were found for homologous BCG (although a 48% reduction in total lesion score was recorded) and BCG-IV (3% reduction). Heterologous regimes did not improve protection over homologous regimes in the wild boar model and showed variable results from no protection to similar protection as homologous regimes. Therefore, homologous regimes remain the best option to vaccinate wild boar against TB. Moreover, vaccine sequence dramatically influenced the outcome underlining the relevance of studying the effects of prior sensitization in the outcome of vaccination.     

广西田园综合体的建设现状和发展建议

文章通过对田园综合体的概念进行介绍，找出广西田园综合体发展情况和存在的问题，最后 提出解决广西田园综合体存在问题的对策和建议，从而促进广西农业的快速发展，带动当地经济稳步 提升。     

低山丘陵区牧草引种和提高山地生物生产力的研究

通过在太行山低山丘陵区的试验研究，系统地总结了在干旱山地人工种草从引种栽培的一系列综合配套技术，从一个侧面反庆子干旱山地综合治理中，牧草与林果的复合栽培在培肥土壤，改良低产农田和提高果园生产力等方面的作用；为在太行山区开展人工种草提供了依据。     

变叶海棠种群多样性的形成与分化研究

 采用杂种指数和形象化散点图法, 探明了变叶海棠以兼性无融合生殖方式与陇东海棠和花叶海棠产生渗入杂交变异, 又以无融合生殖方式保持新产生变异的遗传, 经过数千万年的世代繁衍,形成现今这样极其复杂多样的渗入杂交群体, 该群体为3 个种的杂交复合体。由于杂种的全部或部分能育子代中发生的基因重组和增加基因的突变, 丰富了物种的基因库, 从而深入的揭示了变叶海棠具有多种高抗性的遗传机理。在变叶海棠居群内保护或栽植适量的陇东海棠和花叶海棠, 对于保持和促进变叶海棠多样性的形成与分化具有重要意义。     

结核分枝杆菌分泌蛋白ESAT-6、CFP10基因的克隆、鉴定及其原核表达

以人型结核分枝杆菌 H37RV株基因组 DNA为模板 ,应用 PCR法对 ESAT- 6和 CFP10基因进行扩增 ,产物经纯化后与载体 PMD18- T连接、转化及酶切鉴定 ,亚克隆到原核表达载体 PGEX- 6 P- 1,构建原核重组表达质粒 ,转化入大肠杆菌 BL2 1中 ,以 1mmol/L IPTG诱导 ,进行 SDS- PAGE电泳。结果表明 ,ESAT- 6和 CFP10基因表达的融合蛋白相对分子质量分别为 32 0 0 0和 36 0 0 0 ,与实测相符。重组结核杆菌分泌蛋白 ESAT- 6和 CFP10的成功表达为结核病诊断及重组疫苗的构建打下了基础     

Multisystemic bovine mycobacteriosis in a pony with neurological signs and weight loss

This report describes the clinical, biochemical and histopathological findings of a case of disseminated, primary mycobacterial infection with Mycobacterium bovis in a pony presenting with neurological signs and weight loss. This report revisits tuberculosis as a potential differential diagnosis in horses at a time of relatively strong tuberculosis control in the UK and iterates the importance of post‐mortem examination as a diagnostic tool for clinicians. Key public health questions following exposure to mycobacterial pathogens are also discussed.     

The evaluation of the synergetic degree of ecology economy society system of China forestry during the last decade

Ecological ravages, environmental pollution, and the developmental problems of regional ecology, economy, and society are not geographical restrictions. The synergistic development of the ecology-economy-society system in forestry was quantitatively assessed by system theory and synergistic dynamic models, illustration of the metering principle and corresponding theory, assessing and quantizing synergistic development status of ecology-economy-society system, and the orderliness of the subsystem of the last decade. The orderliness of the ecology and ecological subsystems developed unstably with an increasing trend. The eco-environment construction and eco-conservation achievement of forestry in China are effective and need to be maintained. The industry structure should be improved to promote the growth of the forestry economy. The society subsystem development is increasing but is not at a high level, which indicates that the public demand to ecological service was not satisfied. The theoretical analysis and numerical examples show that the development of the forestry system has a good development state. The synergy changes of forestry system in the 0 to 0.951 range, which is in the intermediate degree of synergy during this period. It still needs much improvement on efficiency of synergy. The orderliness and the relationship among the subsystems of forestry should be improved in future.     

铋系氧化物在光催化技术中的研究进展

总结近年铋系列半导体光催化剂的制备、种类和光催化反应机理等的研究进展,并展望了铋系列半导体光催化剂未来的发展方向.     

Expression,Purification and Activity Evaluation of Mb1230 Gene of Mycobacterium bovis

To explore the value of Mb1230 protein of Mycobacterium bovis in the diagnosis of bovine tuberculosis,we obtained the Mb1230 gene by PCR and constructed recombinant plasmid pET-22b-Mb1230.Recombinant Mb1230 protein was obtained by IPTG induction and purified by affinity chromatography.The activity of the recombinant protein was evaluated by TST test,IGRA test and indirect ELISA.The size of the recombinant protein matched with the theoretical value proved by SDS-PAGE;Western blotting result showed that the recombinant protein could react with mouse anti-His antibody,and had specific band;The results of TST test,IGRA test and indirect ELISA test also showed the recombinant protein had antigenic activity.The results indicated the recombinant protein Mb1230 had good B cell and T cell activity,so,it had the potential application in the diagnosis of bovine tuberculosis.     

烯丙孕素-β-环糊精水溶性衍生物包合物的制备及表征

为了提高难溶性药物烯丙孕素（altrenogest,ALT）的水溶性,本试验采用冷冻干燥法来制备烯丙孕素-羟丙基-β-环糊精（ALT-HP-β-CD）及其衍生物2,6-二甲基-β-环糊精（DIME-β-CD）包合物载药体系,并对ALT的线性关系和专属性、仪器的精密度以及包合物的回收率和稳定性进行了测定。以包合物的收率和包合物中ALT的包合率为评价指标,采用正交试验设计优选2种包合物的最佳制备条件,并使用傅里叶变换红外光谱法、热重分析法和显微镜成像法对所制得的ALT包合物进行表征分析,采用溶解度法测定包合物中ALT的溶解度。结果表明,ALT在1~12 μg/mL范围内具有较好的线性关系且专属性良好,仪器的精密度良好;加入0.1、0.2和0.3 mL ALT标准液的ALT-HP-β-CD包合物的平均回收率分别为97.78%、99.38%和99.90%,加入0.1、0.2和0.3 mL ALT标准液的ALT-DIME-β-CD包合物的平均回收率分别为93.86%、97.72%和94.93%;ALT-HP-β-CD包合物的最佳制备工艺为在55 ℃条件下,ALT与HP-β-CD的投料摩尔比为1∶7,包合时间为4 h,溶液pH为8,溶剂水的加入量为110 mL/g药物;ALT-DIME-β-CD包合物的最佳制备工艺为在55 ℃条件下,ALT与DIME-β-CD的投料摩尔比为1∶4,包合时间为4 h,溶液pH为9,溶剂水的加入量为100 mL/g;经傅里叶变换红外光谱法、热重分析法和显微镜成像法表征,证实成功制得包合物;溶解度试验得出包合物的溶解度分别约为ALT原药的1 012和1 354倍,表明两种包合物均可有效增加ALT的溶解度。本试验包合物的制备方法简单,条件温和,易于产业化生产。