Effects of mosapride citrate, metoclopramide hydrochloride, lidocaine hydrochloride, and cisapride citrate on equine gastric emptying, small intestinal and caecal motility

Objective

Although extensive work has been done to elucidate the beneficial and unfavorable effects of gastrointestinal prokinetic agents in humans, little is known on the effects of these agents in horses. In this study, we compared the effects of mosapride, metoclopramide, cisapride, and lidocaine on equine gastric emptying, jejunal and caecal motility and evaluated these agents’ adverse drug reactions (ADRs).

Animals

Seven healthy adult Thoroughbreds.

Procedure

Mosapride 1.0 mg/kg and 2.0 mg/kg, metoclopramide 0.2 mg/kg, and cisapride 1.0 mg/kg were dissolved in 100 mL distilled water for oral administration. Lidocaine 1.3 mg/kg was mixed with 500 mL saline for a 30-min intravenous infusion. Oral administration of 100 mL distilled water was used as control. Gastric emptying was evaluated using ¹³CO₂ breath test, and jejunal and caecal motility was assessed by electrointestinography.

Results

The present study demonstrates that mosapride at doses of 1.0 mg/kg and 2.0 mg/kg facilitates gastric emptying in horses. Improved jejunal motility was observed following administration of mosapride (1.0 mg/kg and 2.0 mg/kg), metoclopramide (0.2 mg/kg), and cisapride (1.0 mg/kg). Similarly, improved caecal motility was observed following administration of mosapride (2.0 mg/kg).

Conclusions and clinical relevance

This study shows that among the prokinetic agents studied here, only mosapride (2.0 mg/kg) promotes jejunal and caecal motility in horses. Considering mosapride ADRs profile, it is believed that this compound is useful in the treatment of diseases associated with decreased GI motility, including postoperative ileus.     

莫沙必利的妊娠安全性研究

为准确判断孕鼠妊娠日期及给药后流产数和探究莫沙必利对小鼠的妊娠安全性及相关作用机制,将99只小鼠按雌雄比2∶1随机分组进行妊娠造模,根据母鼠体重、外形及阴部变化情况综合判断小鼠妊娠日期,造模成功后将部分妊娠13 d小鼠分为空白对照组、米非司酮加米索前列醇阳性对照组、莫沙必利组,给药后观察整体子宫、胎儿及胎盘发育情况判断莫沙必利对妊娠后期小鼠流产率的影响;将剩余造模成功小鼠随机分为空白对照组、米非司酮加米索前列醇阳性对照组、莫沙必利组,并于妊娠15 d给药后采血,测定血清中缩宫素、孕酮、雌激素、前列腺素含量。结果显示：合笼30 d内共怀孕小鼠60只,其中56只可准确判断其妊娠日期并用于后续试验,占总试验母鼠的84.84%;给药后莫沙必利组胚胎均正常发育,阳性组流产率为100%,空白组流产率为1.79%;激素测定试验中莫沙必利组各项数据与空白组差异均不显著,与阳性对照组相比,血清雌激素,孕酮,前列腺素含量差异极显著。结果表明：试验中所建立的判断小鼠妊娠日期及流产数的方法准确度高且操作简便;初步认为莫沙必利对妊娠后期小鼠的胚胎发育无影响;莫沙必利对部分潜在致孕鼠子宫收缩而造成流产的性激素分泌无影响。