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1.
用两株单克隆抗体混合后包被ELLISA微孔板,加牛冠状病毒抗原,加经白陶土处理过的兔抗牛冠状病毒免血清,再加上辣根氧化物酶标记的羊抗兔IgG抗体,加底物质显色间接ELISA检测牛冠状病毒抗原获得成功。用此方法检测细胞培养的牛冠状病毒上清液,其敏感度高出血凝试验(HA)8倍,从武汉市附近3个奶牛场采集犊牛腹泻粪便105份,用单抗ELISA检测牛冠状病毒抗原,共检出阳性36份,并与血凝及血凝抑制试验, 相似文献
2.
I—ELISA特异诊断棘球蚴(包虫)病的研究 总被引:1,自引:1,他引:0
采用亲和层析技术纯化的兔抗棘球蚴抗体,建立了酶联免疫吸附抑制试验(I—ELISA)。取代反应和阻断反应均无非特异反应;检测健康对照血清126份(人36,猪90),均为阴性;与猪囊尾蚴病血清136份(人46,猪90)、细颈囊尾蚴病血清38份(羊22、猪16)无交叉反应;对棘球蚴病血清183份(人61、羊71、猪51)进行检测,其阳性符合率分别为88.5%(54/61)、98.6%(70/71)、96.1%(49/51)。 相似文献
3.
单抗酶联试剂盒与PCR方法快速检测沙门氏菌的比较 总被引:18,自引:1,他引:17
本文对单抗酶联试剂盒与PCR方法快速检测沙门氏菌进行了比较。将沙门氏菌与大肠杆菌以不同比例混合后 ,分别用ELISA和PCR法检测 ,在 1∶1 0 0的比例时 ,用这 2种方法均能检测出阳性样品 ;在 1∶1 0 0 0的比例时 ,用ELISA法检测为阴性 ,而用PCR法则能检测出。检测鲜牛奶、龙虾、虾仁、熟食制品等样品 ,2种方法的符合率达 97 6 %。对冻虾仁、人粪便样品的前增菌、选择性增菌、后增菌过程进行同步检测 ,在样品的前增菌液 ,直接ELISA法检测的OD值小于 0 5 ,而PCR法扩增能出现特异条带 ,经国标方法验证为阳性 ,证实PCR法的敏感性优于直接ELISA法 ,而在选择性增菌液和M 肉汤后增菌液中 ,2种方法均能检出阳性样品。 相似文献
4.
K. Tagata S. Yokoyama T. Ginbo M. Honda T. Okimura M. Odakura M. Nomura S. Yamamoto 《Veterinary research communications》1996,20(1):21-30
A capillary reversed passive latex agglutination test (capillary RPLA) was developed which allows quantification of serum C-reactive protein (CRP) within approximately 15 min. The logarithmic regression line (calibration curve) obtained after measuring each CRP concentration three times in twofold dilutions of a standard canine serum containing 222 g/ml of CRP was y=6.394+0.030x (r=0.995). Capillary RPLA permitted quantification of CRP in the range 6.9–222 g/ml. The coefficients of variation ranged from 10.28% to 12.40%. The recovery rates (percentage recovery) of CRP by capillary RPLA were within the range 87% to 106%. On measuring the CRP concentrations in sera from 78 dogs by capillary RPLA, single radial immunodiffusion (SRID) and enzyme-linked immunosorbent assay (ELISA), close correlations were demonstrated between SRID and capillary RPLA (y=7.250+1.109x, r=0.978), between SRID and ELISA (y=3.042+1.059x, r=0.967), and between capillary RPLA and ELISA (y=1.778+0.929x, r=0.962). Capillary RPLA may be considered useful as a routine biochemical technique for measurement of serum CRP concentration in the dog.Abbreviations CRP
C-reactive protein
- ELISA
enzyme-linked immunosorbent assay
- RPLA
reversed passive latex agglutination test
- SRID
single radial immunodiffusion 相似文献
5.
6.
晋南冬麦区大麦黄矮病毒流行株系监测及防治策略探讨 总被引:2,自引:0,他引:2
连续5年(1996~2000年)采集晋南冬麦区小麦黄矮病标样,采用生物学和血清学(酶联免疫吸附法)相结合的诊断方法对该地区的大麦黄矮病毒流行株系进行了鉴别。结果表明,该小麦黄矮病流行区近五年以GAV株系为主流株系,兼有少量GPV、PAV和混合株系存在。同时对小麦抗黄矮病新品种“临抗1号”进行了GPV和GAV两种株系的抗性测定,明确了该品种兼抗GPV和GAV两种株系。根据小麦黄矮病发生现状,提出了一套以选育推广抗耐病品种为主,以药剂防治为辅的综合防治措施。以期为当地小麦生产服务。 相似文献
7.
D. Boscia H. Zeramdini M. Cambra O. Potere M.T. Gorris A. Myrta B. Di Terlizzi V. Savino 《European journal of plant pathology / European Foundation for Plant Pathology》1997,103(5):477-480
A monoclonal antibody to an Albanian isolate of plum pox potyvirus (PPV) was obtained (MAbAL), that specifically recognized strain M of this virus. The specificity of MAbAL, assessed by comparative ELISA on 130 PPV isolates of different geographical origin, 22 of which were also tested by comparative IC-PCR, gave consistent and highly reproducible results. MAbAL seems to be elicited by a stable surface determinant that makes it particularly suitable for successful use under a wide range of conditions. MAbAL is an useful addition to the panel of PPV-specific MAbs available to date. 相似文献
8.
Y. Antignus M. Lapidot N. Ganaim J. Cohen O. Lachman M. Pearlsman B. Raccah A. Gera 《Phytoparasitica》1997,25(4):319-330
Received April 24, 1997; received in final form June 29, 1997. Symptoms resembling tomato spotted wilt virus (TSWV) infections
were documented among ornamental and vegetable crops in commercial greenhouses and open fields in Israel. Plants exhibiting
these symptoms were collected from January 1992 to December 1996. Among cultivated plants analyzed for TSWV by enzyme-linked
immunosorbent assay (ELISA), 19 species representing five families were found to be infected; natural infection was also recorded
in six plant species of weeds. Virus identity was characterized by host range, serology and electron microscopy. Serological
reaction with the isolates, found in Israel, using antisera from different sources as well as the sequence analysis of the
nucleocapsid gene, demonstrated that the Israeli isolates of TSWV are a member of tospovirus serogroup I, type I (BR-01 strain).
No virus transmission was found in seeds collected from virus-infected vegetable and ornamental crops. A non-radioactive molecular
probe derived from the cloned nucleocapsid isolate enables specific detection of the virus in crude sap from infected plants.
The detection of TSWV in Israel constitutes a severe potential threat to the ornamental and vegetable industry. 相似文献
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