In vitro and in vivo efficacy of lasalocid for treatment of experimental cryptosporidiosis

In vitro viability of purified Cryptosporidium parvum oocysts, exposed for 30, 60, 90 and 120 min to 0.27 mg/ml lasalocid suspension was evaluated by inclusion or exclusion of two fluorogenic vital dyes and an excystation technique. Continuously, preventive and curative efficacies at different doses (9, 6.75, 5.625 and 4.5 mg/kg body weight) and regimens of lasalocid against cryptosporidial infection were evaluated on an experimental neonatal mice model. In vitro assays demonstrated a decrease in the oocyst viability related to an increase in exposure time for exposure to the lasalocid suspension. The infection was eradicated when the suspension was administered with a dose of ≥6.75 mg/kg body weight. No apparent toxic effects were observed.     

球安等药物抗Eimeria tenella的效力

将１日龄罗曼公雏于无球虫条件下饲养至１３日龄时随机分组,按组分别投予球安９Ａｖａｔｅｃ,含２５％Ｌａｓａｌｏｃｉｄ）１２５ｍｇ／ｋｇ。球佳（含２％Ｄｉｌａｚｕｒｉｌ）１ｍｇ／ｋｇ,加福（Ｃｙｇｒｏ,含１％Ｍａｄｕｒａｍｙｃｉｎ）５ｍｇ／ｋｇ和球净（Ｎｅｃｏｘｉｎ,含２５％ Ｎ ｉｃａｂａｚｉｎｅ和１．６５ Ｅｔｈｏｐａｂａｔａ）１２５ｍｇ／ｋｇ,并设立不给药不感染,不给药感染对照组。     

甜菜碱与三种抗球虫药配伍的增效比较试验

为研究甜菜碱与抗球虫药物配伍抗球虫病的增效作用,进行了疗效比较试验。用甜菜碱(8×104)分别与球安(125×106)、加福(5×105)和球佳(1×106)配伍的3组药物拌入饲料,治疗人工感染E.tenella的鸡,结果各组的平均体增重分别为83.6g、61g和37.5g;盲肠病变值为5.8、8.0和10;ACI为193.7、158.35和126.875。而单用球安、加福和球佳拌料喂的鸡平均体增重分别为82.6g、42g和74g;盲肠病变值8.75、13.25和6.75;ACI为189.375、99.75和180.75。试验表明:甜菜碱加聚醚类抗球虫药组的平均体增重和ACI值均高于单用药组,尤其甜菜碱加加福组增效显著,但甜菜碱加球佳组疗效却降低。     

10株柔嫩艾美耳球虫对四种药物的抗药性检测

从4省市10个鸡场分离到10株柔嫩艾养耳球虫，使用AA肉鸡按每只鸡接种10万卵囊，对马杜霉素、百球清、盐霉素和拉沙里菌素的敏感性进行了测试。依照最适抗球虫活性百分率、相对卵囊产量和病变记分减少率3项指标，检测结果显示：30％虫株对马杜霉素和百球清有抗药性，40％虫株对拉沙里菌素有抗药性，70％虫株对盐霉素有抗药性，80％虫株对两种或两种以上的药物有抗药性。虫株的抗药谱带有明显的地方性。此外，对抗药虫株的鉴定标准进行了讨论。     

三种聚醚类抗生素促进育肥肉牛增重的比较试验

将30头平均体重为435.8kg的夏洛来牛×南阳牛F1分为三组,在饲喂麦秸、啤酒糟和日补精料6.14kg的条件下,分别添加莫能菌素、沙拉里菌素和盐霉素进行饲养试验。结果,莫能菌素、沙拉里菌素和盐霉素组的平均日增重分别为1.20、1.07、1.11kg/d;料重比分别为5.13:1、5.75:1和5.52:1;单位增重的增重剂成本分别为0.073元,0.064元和0.145元。