Tuberculosis vaccination sequence effect on protection in wild boar

The Eurasian wild boar (Sus scrofa) is a reservoir for tuberculosis (TB) in which vaccination is a valuable tool for control. We evaluated the protection and immune response achieved by homologous and heterologous regimes administering BCG and heat-inactivated Mycobacterium bovis (IV). Twenty-one wild boar piglets were randomly allocated in five groups: Control, homologous BCG, homologous IV, heterologous IV-BCG, heterologous BCG-IV. Significant 67% and 66% total lesion score reductions were detected in homologous IV (IVx2) and heterologous IV-BCG groups when compared with Control group (F_4,16 = 6.393, p = 0.003; Bonferroni _{Control vs IVx2} p = 0.026, Tukey _{Control vs IV-BCG} p = 0.021). No significant differences were found for homologous BCG (although a 48% reduction in total lesion score was recorded) and BCG-IV (3% reduction). Heterologous regimes did not improve protection over homologous regimes in the wild boar model and showed variable results from no protection to similar protection as homologous regimes. Therefore, homologous regimes remain the best option to vaccinate wild boar against TB. Moreover, vaccine sequence dramatically influenced the outcome underlining the relevance of studying the effects of prior sensitization in the outcome of vaccination.     

实验动物微生物学质量控制及效果观察

以SPF级（无特殊病原体）动物为种源，采取综合防治措施，在屏障设施饲养清洁级实验小鼠和大鼠。经过两年饲养繁育的结果表明：动物微生物学质量符合清洁级动物国家标准，质量控制效果显著。     

内充气吹式油菜精量排种器气室流场仿真与试验

针对机械式排种器易发生堵塞造成漏播的问题,设计了一种内充气吹式油菜精量排种器。以ANSYS/CFX为工具建立气室流场仿真模型,以气室有无平衡出气孔为试验因素,对气室内气流场进行了仿真分析。仿真结果表明,有平衡气孔的气室压力波动比无平衡气孔小93 Pa。台架试验表明,无气吹作用、无平衡气孔气室气吹作用和有平衡气孔气室气吹作用3种状态下,漏播指数分别为14.4%、4.4%和2.4%,排种器的合格指数分别为66.8%、78.4%和82.0%,在气吹作用下排种器堵塞漏播问题得到明显改善。     

A new lean no time test baking method with improved discriminating power

In response to customer concerns related to gluten strength in commercial baking, the Canadian Grain Commission assessed whether the Canadian Short Process (CSP) test bake method was generating useful data related to intrinsic strength of wheat varieties. Assessment of CSP loaf volume data for Canadian variety trials spanning 2003 to 2013 showed very little correlation with dough strength parameters as measured by farinograph and extensigraph. A lean no time (LNT) test baking method was developed that can better discriminate genotypes and provide objective indicators of the effect of intrinsic dough strength on baking quality. From early method development, through method validation and verification using diverse sets of samples targeting different Canadian wheat classes and grown in three different crop years, results showed the LNT method to be more discriminating and easily adopted by other laboratories. In 2015, the LNT method was adopted as the method of choice in future Canadian variety registration trials. The LNT method is fast, simple and well-suited to high throughput test baking conditions encountered in the evaluation of large numbers of breeder lines. A new objective parameter, loaf top ratio, was also introduced and found to correlate well with dough strength and dough handling properties.     

基于射频卡的高等农林院校计算机实验室管理系统的设计与实现

本文介绍了基于射频卡的计算机实验室管理系统的设计方法与实现技术,主要包括系统分析与设计、网络设计以及系统实施中的关键技术。     

海洋尖尾藻的室内培养

[目的]研究海洋尖尾藻(Oxyrrhis marina Dujardin)室内培养的可行性,为其生理生态特性及其赤潮形成机理的研究奠定基础。[方法]在实验室培养海洋尖尾藻,饵料藻为蛋白核小球藻(Chlorella pyrenoidosa)、湛江等鞭金藻(Isochrysis zhangjiangensis)、亚心形扁藻(Platymonas subcordiformis)和绿色巴夫藻(Pavlova viridis)。[结果]在实验室条件下,除高密度的蛋白核小球藻和湛江等鞭金藻外,以亚心形扁藻和绿色巴夫藻作为饵料时海洋尖尾藻均能良好地生长和繁殖。[结论]该研究为海洋尖尾藻的室内培养解决了饵料问题,实现了海洋尖尾藻室内的长期培养。     

实验室现代化管理系统的构建及运行研究

采用门禁系统、摄像监控系统监控、ZZSHUN智能化设备管理系统、LIMS实验室信息管理系统和搭建网络实验教学平台等现代化管理手段,进行实验室管理和实验教学,在有限的人力资源条件下,实现了实验室管理有序化、规范化、体系化和高效化,提高了实验室的使用效率。     

科研单位大型仪器管理与维护的经验体会

大型仪器设备是科研单位从事科研活动的重要条件和基本手段,也是科研单位综合实力的体现.加强仪器管理与维护,提高仪器利用率已成为当今实验室管理工作的重要研究课题.笔者结合多年工作实践,提出了提升实验室运作绩效的若干措施.     

非洲凤仙茎基腐病病原学及药剂敏感性1）

对非洲凤仙（Impatiens wallerana Hook．f．）茎基腐病的症状进行了描述,结合病原形态特征、培养特性及rDNA－ITS序列分析结果,确定该病害由Rhizoctonia solani Kühn AG4－HG－Ⅰ融合群引起。病菌的生物学特性研究表明：菌丝较适生长温度为25～30℃;菌丝生长较佳的培养基为OA和PSA,较佳的碳源为D－（＋）麦芽糖、α－乳糖和可溶性淀粉,较佳的氮源为硝酸钾和硝酸钠;pH＝7时菌丝生长最好;光照条件对菌丝生长无影响;菌丝致死条件为51℃、10 min。采用生长速率法测定了病菌对30种杀菌剂的敏感性,结果表明,病菌对≥300亿个· g－1内生芽孢杆菌WP、1000亿个· g－1枯草芽孢杆菌WP、25％肟菌·50％戊唑醇WG、40％氟硅唑EC敏感性较高,对其EC50＜1．0 mg· L－1,可以作为田间防治非洲凤仙茎基腐病的首选杀菌剂。     

遗传学实验教材存在的问题及改革实践

基于目前遗传学实验教材一般存在背景知识介绍过于简单且陈旧、操作指导介绍过于笼统等不足,杨大祥在《遗传学实验》编写和修订时作了一些创新：注重实验背景知识、研究思路的介绍及实验结果的解释;注重学生科研基本功的训练;通过课后练习引申实验内容,让学生关注相关技术在实践中的应用等等,以此激发学生探索、发现的兴趣。