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This study aimed at elucidating SS-bonds of HMW-gliadins (HGL) from wheat with the focus on terminators of glutenin polymerisation. HGL from wheat flour extracts non-treated or treated with the S-alkylation reagent N-ethylmaleinimide (NEMI) were compared. HGL from wheat flour Akteur were isolated, hydrolysed with thermolysin and the resulting peptides pre-separated by gel permeation chromatography and analysed by liquid chromatography/mass-spectrometry using alternating electron transfer dissociation/collision-induced dissociation. Altogether, 22 and 28 SS-peptides from samples without and with NEMI treatment, respectively, were identified. Twenty-six peptides included standard SS-bonds of α- and γ-gliadins, high-molecular-weight and low-molecular-weight glutenin subunits. Eleven SS-bonds were identified for the first time. Fifteen peptides unique to HGL contained cysteine residues from gliadins with an odd number of cysteines (ω5-, α- and γ-gliadins). Thus, gliadins with an odd number of cysteines, glutathione and cysteine had acted as terminators of glutenin polymerisation. Decisive differences between samples without and with NEMI treatment were not obvious showing that the termination of polymerisation was already completed in the flour. The two HGL samples, however, were different in the majority of ten peptides that included disulphide-linked low-molecular-weight (LMW) thiols such as glutathione and cysteine with the former being enriched in the non-treated HGL-sample. 相似文献
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Right atrioventricular valve insufficiency and bilateral congestive heart failure were identified in a carpet python (Morelia spilota variegata) with the aid of colour Doppler echocardiography, electrocardiography and radiography. The snake failed to respond to diuretic therapy and was euthanased. Based on this case, it appears that bilateral congestive failure is feasible in univentricular animals with lesions restricted to one side of the heart. Loop diuretic therapy may be inappropriate in non-crocodilian reptiles because reptiles lack a loop of Henle. 相似文献
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应用SpinlabHVI900测试仪和X射线衍射谱的计算分峰法测量了棉纤维品质性状和取向参数。初始弹性模量(M,N/tex)、3.2mm隔距比强(T,gf/tex)、2.5%跨距长度(L,mm)与微原纤螺旋角(φ)、纤维素微晶取向离散角(α)存在如下关系:M=3.73-0.04φ-0.06α,r=0.762;T=58.08-0.30φ-1.33α,r=0.876;L=63.23+0.57φ-1.88α,r=0.905。陆地棉原棉纤维的50%X射线角ψ对φ的比ψ/φ约为2.04,ψ/φ值对于不同棉种具有高度的单一性。 相似文献
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犬细小病毒免疫复合物解离试验的研究 总被引:3,自引:0,他引:3
以犬细小病毒(CPV)做为示范性抗原,以抗犬细小病毒血清(Anti-CPV)做为示范性抗体,制成抗原抗体免疫复合物。探索测定了使犬细小病毒复合物中抗原抗体解离的物理振荡、电解质浓度、缓冲液pH值及作用温度的解离条件。 相似文献
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盐酸解离是果蝇唾腺染色体制片中的关键步骤,研究了盐酸浓度与处理时间对染色体解聚合与制片效果的影响.解离不充分时,压片后唾腺染色体仍团聚在一起;解离过度时,染色体结构受损,二者都达不到制片的要求.用2 mol/L盐酸处理20 min或者用3 mol/L盐酸处理15 min,染色体解聚适度,染色体分散,染色体臂及横纹均清晰可见,制片效果理想.用3 mol/L盐酸解离,所需时间短,更适合于制片观察. 相似文献
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不同酶解条件对普通红豆草子叶原生质体分离的影响 总被引:1,自引:0,他引:1
以普通红豆草子叶为材料,研究了预处理、酶液组合和渗透压浓度等因子对其原生质体分离效果的影响。结果表明,子叶在含有0.70mol/L甘露醇的CPW溶液中预处理60min,获得的原生质体产量、存活率均高于其他预处理;酶液组合为1.0%纤维素酶+0.8%果胶酶+0.5%离析酶,25℃黑暗条件下酶解6h可获得大量有较高活力的原生质体;酶解液中添加浓度为0.55mol/L的甘露醇较适合原生质体分离。 相似文献