Preparation on the basis of Trichoderma asperellum in the system of biological protection of wheat from Fusarium ear scab

During the last century, as the area of wheat grown under advanced grain husbandry has increased worldwide, so too has the importance of Fusarium ear scab (FES) (synonym, Fusarium head blight) caused by several species of the fungus Fusarium. Yield losses due to FES can total 20%-40% and more depending on climatic conditions. During the last twenty years epidemics of FES in cereals have become chronic all over the world, including the United States and Russia. The most destructive of t…     

链霉菌182-2抗真菌活性物质的分离及抑菌特性的初步研究

[目的]链霉菌182-2的发酵液对烟草赤星病有良好的抑制作用,本文拟对其发酵液中抗真菌活性组分进行初步分离,并进行抑菌特性研究.[方法]发酵液经预处理后,依次用活性炭脱色,大孔树脂吸附层析分离纯化,对纯化后活性物质(组分Ⅱ)的抑菌活性进行测定.[结果]分离纯化结果表明其活性物质中至少含有两种抗真菌活性组分.离体条件下当活性物质浓度为2 000μg/mL时,对烟草赤星病菌有很强的抑菌作用,抑菌圈直径达58.0mm.目测法测定抗生作用表明其最小抑菌浓度(MIC)为80 μg/mL,最小杀菌浓度(MBC)为160μg/mL.48 h时对菌丝生长和孢子萌发的抑制率最高,抑菌中浓度(EC50)分别为31.9 μg/mL和42.2μg/mL.活性组分处理还可导致病原菌菌丝和孢子萌发产生的芽管变形扭曲或产生大泡囊.[结论]纯化后的活性物质对烟草赤星病菌具有较强的抑制作用,有进一步研究利用的价值.     

Potential role of G-protein-coupled receptor 30 (GPR30) in estradiol-17beta-stimulated IGF-I mRNA expression in bovine satellite cell cultures

Androgenic and estrogenic steroids enhance muscle growth in animals and humans. Estradiol-17beta (E2) and trenbolone acetate (TBA) (a synthetic testosterone analog) increased IGF-I mRNA expression in bovine muscle satellite cell (BSC) cultures. The goal of this study was to evaluate the mechanisms responsible for this increase by evaluating the effects of ICI 182 780 (an E2 receptor antagonist), flutamide (an androgen receptor inhibitor), G1 (a GPR30 agonist), and BSA-conjugated E2 on E2 and/or TBA-stimulated IGF-I mRNA expression in BSC cultures. Flutamide completely suppressed TBA-stimulated IGF-I mRNA expression in BSC cultures. ICI 182 780 did not suppress E2-stimulated IGF-I mRNA expression and 100nM ICI 182 780 enhanced (93%, p<0.05) IGF-I mRNA levels in BSC cultures. G1 (100nM) stimulated IGF-I mRNA expression (100%, p<0.05) but had no effect on proliferation in BSC cultures. E2-BSA, which cannot cross the cell membrane, stimulated IGF-I mRNA expression (approximately 100%, p<0.05) in BSC but even at extremely high concentrations had no effect on proliferation. In summary, our data indicate the E2-stimulation of proliferation and E2-stimulation of IGF-I mRNA expression in BSC cultures occur via different mechanisms. Our previous results showing that ICI 182 780 inhibited BSC proliferation and results of the current study showing lack of response to E2-BSA or G1 suggest that E2-stimulated proliferation in BSC cultures is mediated through classical estrogen receptors. Stimulation by ICI 182 780, G1 and E2-BSA suggests the E2-stimulated IGF-I mRNA expression in BSC cultures is mediated through the GPR30 receptor.     

Cepharanthine promotes A549 cell apoptosis by regulating miR-150 and miR-182

AIM: To investigate the effect of cepharanthine on the growth of human lung carcinoma A549 cells and the underlying mechanism. METHODS: After A549 cells were treated with cepharanthine, the growth inhibitory rate was detected by MTT assay. The cell morphological changes were observed under light microscope. The apoptosis of the A549 cells was analyzed by flow cytometry. The expression of microRNA (miR)-150, miR-182, p53 mRNA and FOXO1 mRNA were detected by real-time PCR. The downstream target genes were predicted by software, and the expression of p53 and FOXO1 was determined by Western blot. RESULTS: After cepharanthine treatment, the growth of A549 cells was inhibited, the apoptosis rate was significantly increased, and the expression levels of miR-150 and miR-182 were significantly decreased. With cepharanthine treatment at 10 μmol/L, the expression levels of p53 and FOXO1 were elevated; however, with cepharanthine at 30 μmol/L, the expression levels of p53 and FOXO1 were decreased. After transfection with miR-150, the expression of p53 was significantly decreased, while the expression of FOXO1 was significantly decreased after transfection with miR-182. CONCLUSION: Cepharanthine inhibits the growth of A549 cells and promotes the apoptosis of A549 cells by inhibiting the expression of miR-150 and miR-182. miR-150 and miR-182 may down-regulate the expression of p53 and FOXO1, respectively.     

甘蔗品种柳城03/182新植蔗性能表现

为加快甘蔗新品种推广力度,本试验开展了甘蔗新品种柳城03/182在百色蔗区的新植性能试验。结果表明:柳城03/182较新台糖22号表现为早熟、高糖,有效茎数多,但茎径、单茎重不及新台糖22号,比新台糖22号增产3.2%、增糖12%,但差异不显著。柳城03/182适合在百色市蔗区大面积推广应用,有利于改良百色市甘蔗不同熟期品种结构,改变品种单一化格局,为糖厂榨期的合理安排提供有利条件。     

诱导奶山羊发情及解冻精液的配种技术

利用外源激素诱导母羊同期发情效果,对影响冻精配种受胎率的各种因素进行了试验。结果表明,三合激素处理效果显著,注药后48 h100%母羊发情,冷冻精液配种情期受胎率可达51.06%,产羔率220.35%;利用PMSG处理,同期发情效果不如三合激素明显,发情集中在72 h内,发情率85%;利用IC I(80996)诱导母羊发情效果最差,发情率仅达61.67%,并集中在72 h内。同时用不同解冻方法解冻精液后对精子活力、存活时间、顶体完整率、死活染色率进行了对比分析,80℃干解冻最理想(P0.01),活力达0.4,存活时间18 h以上;采用湿解冻时温度应控制在40℃,在实际生产中采用一次性解冻(80℃干解冻或40℃湿解冻)2~3粒冻精,不影响受胎率。     

优质中筋小麦晋太182高产栽培技术研究

为了在山西中部麦区大面积推广优质中筋小麦晋太182,开展了播期和种植密度二因素随机区组试验研究。结果表明,播期与密度对晋太182产量构成因素的影响均达极显著水平,随着播期的推迟,株高依次降低,穗长依次缩短,结实小穗数和不孕小穗数均逐渐减少;随着种植密度增大,植株依次增高、穗长变短、结实小穗数减少、不孕小穗数增多。不同的播期有不同的最佳种植密度。晋太182适播期为9月24—29日,而以9月24日为最佳播期,适宜密度为375万~525万株/hm2,450万株/hm2为最佳密度。晋太182在因前茬作物不能及时收获而晚播的条件下,通过增大种植密度同样可以获得高产。     

基于780MHz频段的温室无线传感器网络的设计及试验

针对以往农用无线传感器网络(wireless sensor network,WSN)能耗与成本较高、传输性能不理想等问题,该文选用无线射频芯片AT86RF212、单片机C8051F920等,设计了一种工作在780 MHz中国专用频段且与IEEE802.15.4c标准兼容的无线传感器网络。该文简述了无线传感器网络节点结构,重点介绍了780 MHz无线传感器网络的硬件设计,并选择北方典型的日光温室作为试验研究环境,通过改变无线收发距离,对780、433和2 400 MHz频段的无线传感器网络节点的接收信号强度值(RSSI,received signal strength index)和平均丢包率(PLR,packet loss rate)进行了测试与分析。试验结果表明,3种不同频段的无线收发模块的接收信号强度值RSSI都随着收发距离的增大而减小。在温室内测试,收发距离小于20 m时,3种无线模块的RSSI值相近;收发距离为40~90 m时,7803 MHz模块比433 MHz模块的RSSI值略大,2.4 GHz的RSSI值最小。在温室内收发距离小于90 m的范围内,780 MHz模块和433 MHz模块的丢包率均为0,2.4 GHz模块的最高丢包率不超过5%。在温室间测试,收发距离为50~90 m时,780 MHz模块和433 MHz模块的RSSI值相近;收发距离大于90 m时,780 MHz模块比433 MHz模块的RSSI值大;2.4 GHz模块在温室间收发距离为50~140 m时的RSSI值均小于433、780 MHz。2.4 GHz模块在收发距离大于70 m时出现丢包现象,收发距离大于135 m时丢包率达到100%;温室间收发距离为140 m时,433 MHz模块的最大丢包率为11%,780 MHz的最大丢包率不超过6%。因此,在温室环境监测的应用中,780 MHz频段的无线传感器网络的传输性能表现最佳,且与433 MHz都明显优于2.4 GHz。     

小麦新品种涡麦182的选育与应用

阐述了小麦新品种涡麦182的选育方法及选育经过,并介绍了涡麦182产量表现突出、产量结构合理、籽粒商品性好、综合抗性好等特点,提出了其栽培技术,以促进该品种的推广应用。     

截短NDV F蛋白的原核表达

根据NDVLaSota株F基因已知的抗原表位,对F蛋白进行分段表达。应用RT—PCR方法分段扩增F基因,并将其克隆到pET30a（＋））原核表达载体上,得到重组质粒pET30-F780和pET30-F760,将质粒导人BL21（ED3）感受态中,经IPTG诱导表达。表达的重组蛋白通过SDS—PAGE和Westem—blotting方法进行鉴定。表达的两段蛋白大小约为31．1kDa和27．9kDa,与预期的蛋白分子量大小相符。Westernblot分析表明重组蛋白可以和NDV抗体发生特异性反应。成功构建了原核表达质粒pET -F780和pET—F760并获得了高效表达,通过Westernblot分析表明重组蛋白具有良好的免疫反应性。