小麦品种抗条锈菌小种专化性估测方法的研究

 在田间设置6品种×4小种组合的小麦条锈病隔离接种试验小区,成株期同步测定了各组合的组织学病变特征数值,包括侵入率、菌落面积、坏死面积、单菌落吸器个数和流行学特征数值,包括相对侵染点数、潜育期、病斑面积、产孢面积、孢子堆密度、病害传播速度和流行速度。计算了各品种,各项特征值的小种间变异系数和品种-小种互作方差贡献率并比较了在抗病性鉴定中利用它们估测小种专化性和非小种专化性的效果。在此基础上进行了多项指标间的相关分析。建议今后在抗病性类型鉴定中,将观察项目简化为镜检坏死面积比、单菌落吸器数或调查侵染几率、孢子堆密度等并计算这些数据的小种间变异系数来定量评价抗病性的小种专化程度。     

Evaluation of newly developed ‘six recombinant secretary proteins based ‘cocktail ELISA’ and ‘whole cell lysate’ based ‘indigenous ELISA’ and tissue microscopy’ with ‘Gold standard’ histo-pathology for the diagnosis of Johne’s disease in slaughtered goats and buffaloes

Three screening tests {(newly developed, six recombinant secretory proteins based 'cocktail ELISA', in-house robust 'indigenous ELISA' based on semi-purified protoplasmic antigens and tissue microscopy were evaluated with 'Gold standard', histo-pathology for the diagnosis of Johne's disease in goats and buffaloes. Serum and tissues {mesenteric lymph nodes and intestines) were driven from farmer's goats (n = 77) and buffaloes (n = 40) slaughtered for harvesting meat and farm goats (n = 77), died and necropsied. Twenty seven (35%) goats and 23 (57.5%) buffaloes were positive in all the four tests. Of 134 tissues screened by histo-pathology, 79.8% MLN and 76.8%, intestines, were positive for MAP infection. In tissue microscopy, 55.2 and 52.3%, goats and buffaloes were positive, respectively. Of 117 sera screened by i_ELISA, 58.4 and 70.0%, goats and buffaloes were positive, respectively. Whereas, c_ELISA detected 55.8 and 62.5%, goats and buffaloes, positives, respectively. Twelve tissues (70.5%) of goats necropsied were positive, both in tissue microscopy and histo-pathology. Most significant gross findings were serous atrophy of the fat and mild to moderate, diffuse thickening of terminal ileum, especially at ileo-caecal junction with or without transverse / longitudinal corrugations. In histo-pathology grade III and IV lesions were significantly low as compared to grade I and II. Of the four tests used for screening 268 samples, histo-pathology was most sensitive (78.3%), followed by i_ELISA (62.3%), c_ELISA (58.9%) and tissue microscopy (58.9%). Between two ELISA tests, c_ELISA using six recombinants secretory proteins, had higher specificity as compared to i_ELISA.