聊城古城景观保护及绿地生态规划研究

基于聊城古城景观结构特色及其建设中存在的问题,依据景观生态学的原理和方法,提出古城景观保护方案并进行绿地系统生态规划。     

佛山市文华公园总体规划

佛山市文华公园是佛山市禅城区中心组团城市景观轴线的重要节点。整个公园采取开放式设计,力图建设成为一个高品质的具有浓郁岭南文化意蕴的“城市绿色客厅”。     

广州旧城区绿地建设对策研究

通过分析城市旧城区绿地建设普遍存在的困难,在建设策略上,提出规划建绿、文化建绿、见缝插绿、立体增绿、临时建绿、拆危建绿、绿地提升、保存大树等针对旧城区绿地建设的对策。     

我国蜂蜜出口中的绿色壁垒影响剖析

近年来我国蜂蜜出口屡屡遭遇发达国家设置的绿色壁垒。对此应该冷静客观审视：正当合理的绿色壁垒对我国蜂蜜出口有着积极的导向作用。从长期来看,这种导向作用必定会促使我国蜂蜜出口竞争力大大提升;但是短期内绿色壁垒对我国蜂蜜出口实际造成一定消极影响。为此,应该采取积极主动的生产与贸易对策。     

脑心肌炎增强型绿色荧光蛋白嵌合病毒的构建

携带增强型绿色荧光蛋白(Enhanced green fluorescent protein, EGFP)的脑心肌炎(Encephalomyocarditis virus, EMCV)嵌合病毒是研究该病毒体内外生物学特性的有力工具。因此,本研究基于前期构建的巨细胞病毒(Cytomegalovirus, CMV)感染性克隆,在EMCV基因组2A蛋白序列之后插入EGFP基因片段,并将重组质粒转染BHK-21细胞,获得携带EGFP的嵌合病毒。通过荧光定量PCR(real-time PCR)、间接免疫荧光(Indirect immunofluorescence assay, IFA)及半数组织细胞感染量测定(Median tissue culture infective dose, TCID₅₀)等方法对拯救病毒的基因组复制、蛋白表达及病毒粒子的感染性进行测定,结果证实嵌合病毒能够在BHK-21细胞上成功表达EGFP并产生完整的病毒粒子。然而,相较于野生型亲本拯救病毒,嵌合病毒在BHK-21细胞上的复制能力降低,并且在连续传代后逐渐失去绿色荧光信号,表明嵌合病毒中EGFP...     

基于HS-SPME-GC-MS的青梅酒香气成分研究

[目的]为更好地勾兑青梅酒,研究青梅浸泡酒、青梅汁、青梅汁发酵酒以及浸泡基酒的香气成分.[方法]采用顶空固相微萃取(HS-SPME)与气相色谱质谱(GC-MS)相结合的方法定性分析浸泡基酒、青梅浸泡酒、青梅汁及青梅汁发酵酒4种样品的香气成分.[结果]试验显示,4个样品依次检测出14、32、17、46种香气成分,香气成分本质的差异决定了其特有的风味和口感.其中,青梅在浸泡过程中产生的特有香气成分为苯甲醛、S-(-)-2-甲基-1-丁醇、苯甲酸乙酯、5-羟甲基糠醛等.青梅汁中特有的香气成分为糠醛、苯乙醇、苯甲醇等.青梅汁发酵酒中产生的主要微量香气成分有苯乙醇(6.941％,峰面积相对百分含量,下同)、异戊醇(6.940％)、辛酸乙酯(3.734％)、癸酸乙酯(2.590％)、己酸乙酯(2.479％)、9-癸烯酸乙酯(2.080％)、5-羟甲基糠醛(1.756％)等.[结论]研究可为青梅酒的品质改善提供科学依据和数据参考.     

黄蟹“和”绿蟹“的鉴别方法及两者的蜕壳死亡率

介绍了中华绒螯蟹蟹种中“黄蟹”和“绿蟹”的鉴别方法，报导了用这两种蟹种进行对照饲养试验的结果。     

Recovery of cover-crop-N in the soil-plant system in the Guinea savannah zone of Ghana

In order to understand the efficiency of residue-N use and to estimate the minimum input required to obtain a reasonable level of crop response, it is important to quantify the fate of the applied organic-N. The recovery of N from ¹⁵N-labelled Crotalaria juncea was followed in the soil and the succeeding maize crop. Apparent N recovery (ANR) by maize from unlabelled Crotalaria juncea, Crotalaria retusa, Calopogonium mucunoides, Mucuna pruriens and mineral fertilizer at three locations were also evaluated. The maize crop recovered 4.7% and 7.3% of the ¹⁵N-labelled C. juncea-N at 42 days after sowing (DAS) and at final harvest, respectively. The corresponding ¹⁵N recovery from the soil was 92.4% and 58.5%. The highest mean ANR of 57.4% was with mineral fertilizer, whereas the mean ANR of 14.3% from C. retusa was the lowest. A large pool substitution and added-N interaction effect was observed when comparing N recovery from the labelled and unlabelled C. juncea. The amount of residue-N accounted for by the isotope dilution method at 42 DAS was 97.1% and at final harvest 65.8%. The large residue-N recovery in the soil organic-N pool explains the residual effect usually observed with organic residue application.     

The Impact of Fertilization Methods on the Nutrient Balance and Changes of Soil Chemical Features in Crop Rotation

The effect of various fertilization methods on the simplified nutrients balance and changes of soil chemical features in crop rotation was estimated on the basis of 7-year (1994-2000) studies carried out on loess-based, sedimentary-gley, light-clay soil. Different fertilization methods were used: A - mineral fertilization; B - mineral and manure fertilization; C - straw, green manure and mineral fertilization; D - ecological compost and biodynamic preparation fertilization. Particular fertilization methods caused a significant differentiation of the nutrients balance. A positive phosphorus balance was found out in all the fertilization systems. It has been observed that the nitrogen and potassium carry-in with fertilizers was higher than the carry-out with crops in system B and C. A favorable calcium balance was achieved in systems C and D while a favorable magnesium balance - only in the ecologically fertilized systems (D). The changes of soil chemical features were only insignificantly dependent on the nutrient balance. After seven years of research, the contents of humus, phosphorus, potassium, magnesium and calcium increased while the soil reaction decreased in the soil of all the fertilized systems except the ecologically fertilized system.     

基于城市生态安全的防护林体系建设探讨——以广州“绿色亚运”增绿行动计划防护林建设为例

城市防护林建设是城市安全重要内容,建设好城市防护林对城市可持续发展有着重要意义.广州“绿色亚运”增绿行动防护林建设是系统论、景观生态学理论的融合,是践行建设生态高效、景观优美、城乡一体、以人为本的宜居城市防护林体系.文章在对广州防护林现状分析的基础上,从沿海防护林、工业园防护林、农田林网三大建设工程方面对广州“绿色亚运...