PRRSV弱毒细胞疫苗与灭活疫苗生殖道黏膜免疫效果观察

应用组织学方法比较观察了猪繁殖与呼吸综合征病毒(PRRSV)弱毒细胞疫苗与灭活疫苗生殖道黏膜免疫的效果。试验选用5月龄长白×大约克夏二元杂交母猪18头。PRRSV弱毒细胞疫苗和灭活疫苗通过生殖道黏膜免疫接种。结果表明PRRSV弱毒细胞疫苗生殖道黏膜接种组母猪的子宫黏膜上皮内淋巴细胞数较灭活疫苗生殖道黏膜免疫接种组和对照组母猪显著减少(P<0.01);而且子宫黏膜上皮变矮变薄,变得有些不规则。灭活疫苗生殖道黏膜接种组母猪子宫黏膜上皮内淋巴细胞数与对照组母猪间差异不显著(P>0.05)。本试验结果显示PRRSV弱毒细胞疫苗经生殖道黏膜免疫接种则对生殖道黏膜上皮内淋巴细胞产生抑制作用。说明PRRSV弱毒细胞疫苗和灭活疫苗经生殖道黏膜免疫效果不理想     

Mortality,morbidity, and external injuries in piglets housed in two different housing systems

An investigation was undertaken to study two different types of farrowing pens, use being made of well characterized veterinary parameters for the well‐being of piglets. A housing system with a solid floor and straw‐bedding was compared with a partly metal‐slatted floor system. The veterinary parameters we used were mortality, morbidity, and external injuries in piglets. There were slight differences in mortality and morbidity rates between the two housing systems. There were big differences with regard tp the piglets” injury‐index’ of the two housing systems. The straw system was more favourable. The studies show that obervations of external injuries ('Method of Ekesbo') can be used to judge housing systems of piglets in the farrowing house.     

Genomic characterization and comparative analysis of Leptospira kirschneri serogroup Grippotyphosa UC5/2011, a strain isolated after mare abortion: Implications for genital animal leptospirosis

The genome of a Brazilian strain of Leptospira kirschneri serogroup Grippotyphosa isolated from a mare post-abortion was sequenced and analyzed. High symmetrical identity and few structural differences were found when compared with a European strain of the same serogroup, L. kirschneri serovar Valbuzzi strain 200702274. Genes associated with virulence and antimicrobial resistance were found. Knowledge of the virulence evolution of Leptospira remains limited, especially in diseases of the reproductive sphere. We highlight the importance of virulence studies in the sphere of genital leptospirosis.     

Insertion sequence profiling of UK Mycoplasma bovis field isolates

Mycoplasma equigenitalium and Mycoplasma subdolum have been associated with infertility, endometritis, vulvitis and abortions in mares, and with reduced fertility and balanoposthitis in stallions. Despite their role in equine genital disorder, determinants of virulence and pathogenesis as well as factors provoking specific host immune responses have not been identified, so far. To establish the major immunogenic components of Mycoplasma (M.) equigenitalium and M. subdolum, antigen profiles of their type strains as well as 30 clinical isolates were compared by SDS-PAGE and immunoblot analysis using hyperimmune rabbit sera and equine sera from clinical cases. To define the major protein antigens of both mycoplasma species, detergent-phase fractionation with Triton X-114 was performed. Western blot analysis of 30 clinical isolates revealed a high similarity of their overall antigen profile with only slight differences. In contrast, monospecific polyclonal antibodies raised against detergent-phase proteins of the two mycoplasma species identified three prominent proteins (pST17, pST42, and pET45) undergoing variation in expression and size among clinical and clonal isolates.     

PRRSV弱毒细胞疫苗与灭活疫苗生殖道黏膜免疫效果观察

应用组织学方法比较观察了猪繁殖与呼吸综合征病毒(PRRSV)弱毒细胞疫苗与灭活疫苗生殖道黏膜免疫的效果。试验选用5月龄长白×大约克夏二元杂交母猪18头。PRRSV弱毒细胞疫苗和灭活疫苗通过生殖道黏膜免疫接种。结果表明PRRSV弱毒细胞疫苗生殖道黏膜接种组母猪的子宫黏膜上皮内淋巴细胞数较灭活疫苗生殖道黏膜免疫接种组和对照组母猪显著减少(P＜0.01);而且子宫黏膜上皮变矮变薄,变得有些不规则。灭活疫苗生殖道黏膜接种组母猪子宫黏膜上皮内淋巴细胞数与对照组母猪间差异不显著(P＞0.05)。本试验结果显示PRRSV弱毒细胞疫苗经生殖道黏膜免疫接种则对生殖道黏膜上皮内淋巴细胞产生抑制作用。说明PRRSV弱毒细胞疫苗和灭活疫苗经生殖道黏膜免疫效果不理想。     

Review papers

The occurrence of keratoconjunctivitis infectiosa ovis (KIO) in the Netherlands was reported by Holland et al. in 1969 for the first time. At present the disease is well known in the Netherlands. Nevertheless there are still questions about the causative agent and the most effective and easiest therapy.

Most authors suppose that the disease is caused by Colesiota conjunctivae, although others mention infections by other chlamydia, Mycoplasma conjunctivae, Mycoplasma ovipneumoniae, Acholeplasma oculi and a wide variety of bacteria.

The diagnosis can be made on the basis of the symtoms and the detection of the agent in Conjunctival scrapings. The bacilliform bodies can be found in conjunctival smears in the cytoplasm (Giemsa, Stamp).

Many therapies are used topically, parenterally or orally. Locally used eye‐ointments must be effective against Colesiota; antimicrobial drugs administered by injection must be effective against the latter and also provide a sufficient cell tissue penetration with excretion into the lacrimal fluid. Injections have proved to be easier to administer, especially in serious outbreaks, but such outbreaks are exceptional (18). Some therapies have been evaluated in small scale experiments (28) as well as in field trials (17).     

荣昌公猪生殖器官的解剖学观察

解剖了5头荣昌公猪的生殖器官,其年龄为1岁左右,体重90公斤。观察和测量了睾丸、附睾、副性腺和尿生殖道等器官。 睾丸平均重为53±13.9克,附睾均重29.8±8.6克,睾丸输出管平均数为14.5±3.8条,输精管平均长46.3±2.3厘米。副性腺平均重量如下:精囊腺102±37.8克,前列腺体部8.5±2.5克,尿道球腺89±15克。尿生殖道骨盆部平均长17.6±1.29厘米。尿生殖道阴茎部均长45.7±2.4厘米。阴茎平均长46.3±2.3厘米。包皮盲囊的平均大小如下:长3.8±0.2厘米、宽5.2±0.55厘米、高3.9±0.6厘米。睾丸动脉均长为110±19厘米。精索淋巴结的出现率为40％,其体积差异很大。