A型口蹄疫病毒VP1蛋白间接ELISA检测方法的建立

以原核表达经纯化复性制备的A型口蹄疫病毒(FMDV)VP1蛋白代替传统病毒抗原包被,建立快速、安全、有效的A型FMDV抗体ELISA检测方法。对前期制备的A型VP1蛋白进行Western-Blot检测,结果表明,VP1蛋白能够特异性识别A型FMDV阳性牛血清,可作为检测抗原建立检测A型FMDV抗体的方法。以最佳质量浓度1mg/L VP1蛋白为检测抗原,方阵滴定法确定最佳检测血清稀释度为1∶50[V(血清)∶V(封闭液)],最佳的酶标二抗稀释度为1∶2 000[V(酶标二抗)∶V(封闭液)],建立A型FMDV抗体ELISA检测方法。该方法的敏感性为94.32%,特异性为99.09%,批内与批间重复试验变异系数均小于8%。采用该方法检测201份临床血清,与液相阻断ELISA试剂盒的符合率达92.54%。研制的VP1-ELISA试剂盒特异、敏感、稳定、操作简便,可用来监控A型口蹄疫抗体水平。     

高产奶牛血清镁含量分析

为调查南宁地区某奶牛场奶牛血清镁含量及其是否与该场奶牛蹄病有关,选取40头健康奶牛和46头蹄病奶牛,采用原子吸收分光光度法分析每头牛血清镁含量,结果表明全部被检牛血清镁平均含量为22.72±3.11mg/L;健康组与蹄病组奶牛血清镁含量分别为23.02±3.19mg/L和22.45±3.09mg/L,两组间差异不显著(P＞0.05).     

云南省水稻细菌性基腐病病原的初步鉴定

 2000年从云南省楚雄市采到水稻细菌性基腐病标本,经病害症状观察,细菌分离培养,革兰氏染色,鞭毛染色,致病性测定及致死温度测定的结果,基本上与前人^［1］报道的相符,其病原菌初步鉴定为菊欧氏杆菌（Erwinia chrysanthemi)。     

Social-ethical issues concerning the control strategy of animal diseases in the European Union: A survey

In 2004 a survey was conducted in the member states of the European Union designed to gain greater insight into the views on control strategies for foot and mouth disease, classical swine fever, and avian influenza with respect to the epidemiological, economic and social-ethical consequences of each of these animal diseases. This article presents the results of the social-ethical survey. A selection of stakeholders from each member state was asked to prioritize issues for the prevention and control of these diseases. A majority of stakeholders chose preventive measures as the preferred issue. An analysis was done to determine whether there were differences in views expressed by stakeholders from member states with a history of recent epidemics and ones without such a history, and whether there were regional differences. There were no differences between member states with or without a history of recent epidemics. There were indeed regional differences between the priority orders from Northern and Southern Europe on the one hand, and from Eastern Europe on the other. Nina E. Cohen is a biologist and is a researcher at the Wageningen University. She is specialized in societal and ethical issues in human–animal relationships. Her current research is focused on the social-ethical issues concerning the prevention and control of foot and mouth disease, classical swine fever and avian influenza. Marcel A.P.M. van Asseldonk has studied animal science. Currently he works at the Institute for Risk Management in Agriculture (IRMA) of the Wageningen University. He is specialized in the design and pricing of insurance policies and animal health funds for the main livestock epidemics. Elsbeth N. Stassen is a veterinarian and professor of Animals and Society at the Wageningen University. Elsbeth Stassen is specialized in animal health, animal welfare and human–animal relationships. She was a member of a governmental welfare committee during the avian influenza epidemic in the Netherlands in 2003.     

口蹄疫病毒2c基因的克隆及表达

从O型口蹄疫病毒China98株病毒材料中克隆到了非结构蛋白2C的基因，并成功地将该基因在大杆菌中进行了表达，为建立以基因工程产品为抗原，能区分人工免疫和自然感染动物的检疫方法提供也技术和材料条件。 sri     

牛烂蹄坏尾病致病弯角镰刀菌代谢产物分离鉴定

取金寨县耕牛烂蹄坏尾病致病弯角镰刀菌的玉米培养物，用丙酮提取，硅胶净化，制备型硅胶大板分离，得到无色针状结晶体，经质谱和红外光谱分析，并经相关谱图数据库联机检索、甄别，按其分子量、化学式及结构式确定为麦角甾醇，从而证实了致牛烂蹄坏尾病的弯角镰刀菌可产生麦角甾醇。     

三种试剂盒检测猪口蹄疫O型抗体的对比试验

使用三种猪口蹄疫O型抗体检测试剂盒分别检测接种猪口蹄疫O型合成肽疫苗和猪口蹄疫O型灭活疫苗的免疫抗体,以探讨三种抗体检测试剂盒的相关性。结果发现,猪口蹄疫病毒VP1结构蛋白抗体ELISA试剂盒和猪口蹄疫O型液相阻断ELISA试剂盒都可检测猪口蹄疫O型合成肽疫苗,两种试剂盒的相关性达90.8%,而间接血凝试剂盒不能用来检测猪口蹄疫O型合成肽疫苗;猪口蹄疫O型液相阻断ELISA试剂盒和间接血凝试剂盒可用来检测猪口蹄疫O型灭活疫苗,两种试剂盒的相关性达93.7%,而猪口蹄疫病毒VP1结构蛋白抗体ELISA试剂盒不能用来检测猪口蹄疫O型灭活疫苗。     

Ultrasonography of the Collateral Ligaments of the Distal Interphalangeal Joint in Horses: Technique and Reference Images

This study describes ultrasound (US) technique and reference images of the equine distal interphalangeal joint collateral ligaments (CLs), and evaluates the portion of the CLs assessable by US in a series of normal forelimbs. Transverse and longitudinal US images were obtained on five healthy horses and on 25 equine cadaver forelimbs. On six limbs, a needle was placed under US-guidance at the distal limit of visualization of each CL, and the portion of CL visible at US was evaluated on computed tomographic (CT) images. The normal CLs appear as oval structures located abaxial to the fossae of the middle phalanx in the transverse sections, obtained at the level of the coronary band. A centrodorsal hypoechoic image appears with increasing proximodistal probe inclination, demonstrating different fiber orientations within the ligament. Two main fascicles, a deep and a superficial, distally divergent, are visible on longitudinal images obtained in the central part of the ligament. The proportion of CL visible at US examination was more than 50% of the total ligament length in nine of the 12 CLs assessed by CT. Awareness of the estimated portion of distal interphalangeal joint CLs visible at US and detailed knowledge of the US technique and CLs morphology are essential to efficiently use US examination on clinical cases.     

口蹄疫及检测技术进展

口蹄疫包括七个主要的血清型,被国际兽疫局定为一类传染病,我国农业部也将其列为一类传染病。因为近年频繁爆发,严重威胁到畜禽生产和人类自身生存安全,同时由于亚型多、抗原变异性强、宿主范围广等原因,其生态学和公共卫生学意义有极其重要的作用,本文就它的感染机制和各种诊断与防制措施进行概述,从而阐述目前口蹄疫的诊断技术进展状况和进展。