“无饲养细胞”杂交瘤制备技术

在３７℃温度下，用４５％ＰＥＧ（ｐＨ７．４）对ＮＳ０瘤细胞和免疫Ｂａｌｂ／ｃ鼠脾脏细胞做１ｍｉｎ融合。细胞融合后，加入１６４０／ＨＡＴ培养基，在不加饲养细胞和预加饲养细胞的两种环境中培养。结果发现融合细胞培养中不加小鼠腹腔巨噬细胞，杂交瘤克隆孔形成的量较预加小鼠腹腔巨噬细胞的多。     

哺乳动物胚胎干细胞分离与培养的研究进展

从胚胎干细胞的来源、分离方法和饲养层细胞等方面,总结了胚胎干细胞分离与培养在近年来所取得的进展。     

支持牛类胚胎干细胞发育的饲养层培养体系的建立

以小鼠胎儿和牛睾丸为材料 ,以含 1 5 % NBS、0 .1 mmol/ Lβ-巯基乙醇、0 .1 μmol/ L Na2 Se O3的DMEM溶液为培养液 ,分离获得了传 1 5代的小鼠胎儿成纤维细胞和 5代牛睾丸成纤维细胞 ,建立了小鼠和牛类 ES细胞培养体系。结果表明 :牛睾丸成纤维细胞和小鼠胎儿成纤维细胞均属附着生长型细胞 ,与小鼠胎儿成纤维细胞相比较 ,牛成纤维细胞直径和长度大 ,生长速度快 ,易于老化 ;1 2～ 1 6日龄的小鼠胎儿最适宜分离与克隆小鼠胎儿成纤维细胞 ;在 2 5℃条件下 ,以 0 .2 5 %胰蛋白酶 0 .0 4% EDTA消化液作用胎儿小块组织分离原代小鼠胎儿成纤维细胞 ,消化液作用时间不应超过 2 0 min,以相同的消化液在 3 7℃条件下 ,离散贴壁成纤维细胞 ,作用时间以 2～ 3 min为宜 ;培养细胞密度与传代时间间隔有密切关系 ,若传代时间间隔为 3～ 4d,培养细胞浓度应为 3× 1 0 5个 / ml～ 5× 1 0 5个 / ml;在成纤维细胞分离与克隆过程中 ,培养基中添加0 .1μmol/ L Na2 Se O3 0 .1 mmol/ Lβ-巯基乙醇 1 5 % NBS,有利于 MEF和 NBTF的增殖     

One or Two Feeders for Groups of 16 Growing–Finishing Pigs: Effects on Health and Production

Over a 3-year period, seven batches of growing-finishing pigs in groups of 16 pigs (total 672 pigs) were fed using one or two singlespace feeders. The pigs with access to two feeders had a significantly lower number of skin lesions (P<0.0001) and higher daily weight gain than did the pigs with access to one feeder (863 vs. 837 g day- 1 , P<0.05). The within-pen variation in daily weight gain was higher in the pens with one feeder than in the pens with two feeders (SD 139 vs. 106g day- 1 , P<0.0001). The pigs with access to two feeders consumed more feed during the period when they were allowed to eat ad libitum , in comparison to the pigs with access to one feeder (2.07 vs. 1.97 kg day- 1 , P<0.05). The feed conversion ratio of the pigs did not differ between the two treatments. No differences in carcass meat percentage, health or number of remarks at slaughter were detected. A comparison was made between using computerized IVOG® feeders, with individual recording of feed intake, and conventional feeders. The feed conversion ratio was poorer for the pigs fed with the IVOG-feeders than for those fed with conventional feeders. The automatic recordings from the IVOG-system showed that there had been a higher daily feed intake during the ad libitum period. During the restricted period the pigs had a lower daily weight gain than with the conventional feeders.     

稻壳气力给料装置试验研究

为可靠输送稻壳,提出了一种可用于小型稻壳气化或燃烧装置的非机械阀气力给料方案。通过试验考察了给料系统的输送能力、调节范围及输送效率等方面的性能,结果表明,该方案低负荷调节特性好,给料量调节范围宽,输送效率高,气固比小,与机械式给料方案相比,结构简单,成本低,运行可靠,有较好的推广、应用前景。     

Efficacy of the Boar-Operated-System to deliver baits to feral swine

Feral swine (Sus scrofa) pose a significant disease threat to livestock and humans. Emerging technologies to reduce feral swine disease transmission risks include fertility control, vaccination, and toxicants. However, for these technologies to be appropriate for field application, a feral swine-specific oral delivery system is needed. We used two field trials to generate information related to appropriate field application of the Boar-Operated-System (BOS?), an oral delivery system designed to provide bait access only to feral swine. Our objectives were to determine whether pre-baiting BOS? units increased bait removal and to evaluate the proportion of feral swine and non-target animals that ingest baits designed to deliver pharmaceuticals through the BOS?. During both trials we used baits housed within 10 BOS? units. We monitored wildlife visitation, bait removal, and ingestion using motion sensing digital photography and baits containing the bait marker tetracycline hydrochloride (TH). During trial 1 we found three of five pre-baited BOS? units were used by feral swine only. Additionally, we found the five BOS? units that were not pre-baited were not used by feral swine or non-target wildlife. During trial 2 we determined bait removal from the BOS? to be reduced by only 10% for feral swine when activated, whereas bait removal from the BOS? by all other wildlife was reduced by 100% when activated. We captured 81 feral swine and 23 raccoons and found 90% and 13% to have TH-marked teeth, respectively. With minor modifications, the BOS? should be considered a valuable tool to be used in feral swine disease management in conjunction with existing technologies.     

A Comparison of Three Conventional Horse Feeders with the Pre-Vent Feeder

This study compared a feeder with molded cups on the bottom (Pre-Vent feeder) with a commonly used rubber tub and hanging bucket feeder to determine differences in time spent eating and feed wastage. Nine Quarter Horse geldings were fed a 12% crude protein pellet diet at 0.75% body weight twice daily from one of the three feeders twice daily for 3 days, and then switched to the next feeder, following a 3 × 3 replicated Latin square design (n = 9). The horses spent more time eating from the cup feeders (31.15 ± 1.43 minutes) than from bucket (19.39 ± 0.42 minutes) and tub (18.87 ± 0.49 minutes, P < .0001) feeders. When fed from cup feeder, horses dropped significantly less feed (3.2 ± 0.52%) of their ration than when fed from bucket (10.2 ± 1.75%) and from tub (7.0 ± 1.32%, P = .001) feeders. When the most wasteful horse was fed from the cup feeder, he lost a mean of 8.7% of his ration, compared with 32.8% when fed from the bucket, and 26.2% when fed from the tub feeders. The cup design is useful for increasing time spent eating and reducing feed wastage.     

竖直下降管换热实验台改进设计与实

下降管式热解液化装置能够实现生物质热解液化.为了研究下降管中生物质半焦和陶瓷球两种散体混合下落的换热规律,在前期研究的基础上,对实验设备进行了改进设计,并将原实验物料--生物质粉更换为生物质半焦,使实验结果对实际装置的设计与改进更具指导意义.根据不同温度陶瓷球(50、60、70℃)与室温空气的换热实验,通过热平衡分析,得到了陶瓷球热载体与空气的对流传热系数h=600 W/(m2*K);陶瓷球与生物质半焦颗粒换热实验表明,不考虑边界效应时竖直管内温度呈线性分布;对流换热为竖直下降管系统热量传递的主要方式.     

血清和饲养层对水牛精原干细胞体外培养的影响

本研究首先将水牛睾丸经二步酶消化法制成单细胞悬液,依次采用差速贴壁法和Percoll不连续密度梯度离心法分离和纯化精原细胞。在制备好的小鼠胎儿成纤维细胞饲养层上,采用含2．5％血清的培养液对精原细胞进行体外培养,观察血清和成纤维细胞对精原细胞生长的影响,并在体外成功培养了2周通过提取体外培养精原干细胞总FZNA,设计引物并对其进行基因鉴定和免疫细胞化学鉴定,证实体外培养所得的细胞仍保持有精原干细胞的特性,并且该克隆是处在未分化状态的精原干细胞形成的。上述研究可为体外建立水牛精原干细胞长期培养体系提供技术支撑。     

小型循环式家蚕人工饲料给料机的研制

探讨了挤压式人工饲料给料法 ,并研制了一种小型循环式人工饲料给料机。试验结果表明 ,该机具有结构简便、效率高、可靠性好的特点 ,可取代家蚕人工饲料育给料工序的手工作业 ,给料工效达 10 0kg/h ,为手工操作的 10倍以上。给料工艺符合人工饲料育技术要求。