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Thirlwall RE Commander NJ Brew SD Cutler SJ McGiven JA Stack JA 《Veterinary research communications》2008,32(3):209-213
This report describes the use of cell mediated immunity to improve specificity of current diagnosis for Brucella suis. Diagnosis is problematic due to cross reactions that lead to false positive serological reactions (FPSR) in the standard
diagnostic tests. A common cause of this cross reactivity is infection with the organism Yersinia enterocolitica O:9. Gottingen™ mini-pigs were experimentally infected with B. suis biovar I field strain or Y. enterocolitica serotype O:9 biotype 3. Infection was followed for 70 days. During this time whole blood stimulation assays were set up using
Brucella specific antigen. IFNγ was measured in the supernatants (SN) from these assays by ELISA. Concurrent standard serological
tests were carried out. The results indicate that the IFNγ assay is specifically able to distinguish Y. enterocolitica O:9 infection from a B. suis infection in experimentally infected mini-pigs. These results represent an improvement in diagnostic specificity compared
to currently used serological tests. Thus suggesting that in a surveillance setting this test could be applied as a confirmatory
test in the face of FPSR.
The authors are British Civil Servants and as such their work is subject to British Crown Copyright. This means the exclusive
copyright for the article cannot be transferred. 相似文献
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