FABPs作为猪肌内脂肪沉积候选基因的研究进展

对FABPs 作为猪肌内脂肪沉积候选基因的研究现状及前景作一综述。主要包括H-FABP和A-FABP基因的性质、变异以及与肌内脂肪含量的关系。     

藏猪FABPs基因PCR-RFLP多样性研究

采用PCR-RFLP技术检测藏猪H-FABP和A-FABP基因遗传多态性。结果发现藏猪H-FABP-HinfⅠ酶切出现3种基因型,其中基因型HH频率最高,为63.39%;A-FABP-BsmⅠ酶切出现3种基因型,其中基因型AA频率最高,为55.36%;上述基因型分布均符合Hardy-Weinberg平衡。藏猪这2个基因均呈多态分布,与高IMF含量相关的基因型频率较高,这可能与藏猪优良的肉质有关系。     

Anti-diabetic effects of emodin involved in the activation of PPARγ on high-fat diet-fed and low dose of streptozotocin-induced diabetic mice

Rheum palmatum Linn has been widely applied in the clinical treatment of diabetes mellitus. It has been found that emodin as the major bioactive component of R. palmatum L exhibits the competency to activate peroxisomal proliferator-activated receptor-γ (PPARγ) in vitro. So the aim of this study was to evaluate the anti-diabetic effects of emodin through the activation of PPARγ on high-fat diet-fed and low dose of streptozotocin (STZ)-induced diabetic mice. The diabetic mice were intraperitoneally injected with emodin for three weeks. No changes of food consumption and the body weight in emodin-treated mice were monitored daily during the entire experiment. At the end of experiment, the levels of blood glucose, triglyceride and total cholesterol in serum were significantly decreased after emodin treatment. However, serum high-density lipoprotein cholesterol (HDLc) concentration was significantly elevated. The glucose tolerance and insulin sensitivity in emodin-treated group were significantly improved. Furthermore, the results of quantitative RT-PCR analysis showed that emodin significantly elevated the mRNA expression level of PPARγ and regulated the mRNA expressions of LPL, FAT/CD36, resistin and FABPs (ap2) in liver and adipocyte tissues. No effects on the mRNA expressions of PPARα and PPARα-target genes were observed. Taken together, the results suggested that the activation of PPARγ and the modulation of metabolism-related genes were likely involved in the anti-diabetic effects of emodin.     

脂肪酸结合蛋白（FABPs）基因多态性对畜产品品质的影响

脂肪酸结合蛋白（FABPs）属于脂结合蛋白超家族成员，广泛存在于动物细胞质中，承担着细胞内脂肪酸的运输任务。研究发现，脂肪酸结合蛋白（FABPs）基因多态性可以通过调控脂肪代谢来改善畜产品品质，提高饲料利用率，降低环境污染。就FABPs的结构和类型，FABPs对脂肪代谢的影响，FABPs基因多态性及其应用后在改善畜产品品质中发挥的作用、功效和应用特点进行了阐述，以期为改进畜禽养殖技术提供参考。     

宁乡猪FABPs、SLC13A5和NR1H4基因多态性及其与IMF含量的关联分析

旨在研究宁乡猪FABPs、SLC13A5和NR1H4基因的多态性及其与肌内脂肪（IMF）含量的关联性。本试验采用PCR-RFLP和Sanger测序的方法对172头宁乡阉公猪FABPs、SLC13A5和NR1H4基因的多态性进行检测,并探究多态性与宁乡猪IMF含量的关联性。结果表明,宁乡猪H-FABP-Hinf Ⅰ酶切位点存在多态性,其中Hh基因型个体的IMF含量最高,与HH、hh基因型个体的IMF含量差异极显著（P<0.01）;H-FABP-Hae Ⅲ酶切只检测到DD和Dd两种基因型,Dd基因型个体的IMF含量极显著高于DD基因型个体（P<0.01）;H-FABP基因的Hinf Ⅰ和Hae Ⅲ多态性位点共形成3种单倍型和5种单倍型组合,其中单倍型组合HD/hd个体具有最佳的IMF含量。H-FABP的Msp I酶切结果及A-FABP基因测序结果显示,宁乡猪不存在多态现象;SLC13A5基因多态性位点g.50705299T/C和NR1H4基因多态性位点g.83607915G/A处不同基因型间IMF含量的差异均不显著（P>0.05）。综上所述,H-FABP基因Hinf Ⅰ和Hae Ⅲ酶切位点多态性与宁乡猪IMF含量显著关联,其形成的单倍型组合HD/hd的IMF含量最高。以上结果可为宁乡猪IMF含量性状的早期分子选育提供参考。