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排序方式: 共有57条查询结果,搜索用时 15 毫秒
1.
斑马蛔虫ITS及5.8SrDNA的克隆及进化分析 总被引:1,自引:0,他引:1
研究从斑马体内分离的蛔虫的核糖体DNA内转录间隔区(ITS)及5.8SrDNA序列的遗传变异情况,并用ITS序列重构斑马蛔虫与其它蛔虫的种群遗传关系。利用聚合酶链反应(PCR)扩增斑马蛔虫rDNA的ITS-1、5.8S及ITS-2片段,将PCR扩增出的片段纯化后克隆至pGEM.TEasy载体,重组质粒通过菌落PCR鉴定后,对阳性菌落进行序列测定并进行序列分析。结果显示所获得的斑马蛔虫ITS及5.8SrDNA序列总长为892bp,包含部分的18S、28S及全部的ITS,1、5.8S及ITS.2序列。证实从斑马体内分离的蛔虫为马副蛔虫,从而为斑马蛔虫的分类鉴定以及进一步的分子流行病学调查奠定了基础。 相似文献
2.
The practice of horse rugging has important implications for horse welfare and performance, but in Australia, rugging practices have not been properly documented. The objective of this study was to obtain an understanding of Australian horse rugging practices. An online survey reviewed the management of 2659 Australian horses over 12 months. Of the reported horses a total of 84.8% were rugged, although most owners (69.5%) indicated that they were unsure whether horses needed to be rugged at all. Rugging was felt by 59.8% of owners to be a requirement for horses in competition. Riding and competition were significantly associated with the use of rugs. Nearly all respondents (89.0%) felt that ‘over-rugging’ was a concern. However, 4.8% of owners would use up to 4 rugs at any one time on their horse and 21.4% of horses were still rugged in temperatures above 20°C in Australia. Many Australian horse owners (42.8%) also believe that horses feel cold if they are not rugged. Anthropomorphism when it comes to rugging horses cannot be justified as the horse has a much wider thermoneutral zone (TNZ) than humans. Australian climate, owner opinions, equestrian discipline and whether a horse is used for riding influence Australian horse rugging practices. However, current practices are based on limited available research and are not necessarily to the benefit of the horse. The major limitation was that the survey likely attracted respondents who are interested in the topic creating a self-selection bias. 相似文献
3.
Tonometry was performed to estimate intraocular pressure (IOP) in 12 Nubian ibexes ( Capra ibex nubiana ), 10 Grant zebras ( Equus burchelli ) and five Arabian oryxes ( Oryx leucoryx ), using both applanation (Tono-Pen) and/or indentation (Schiotz) tonometers. Animals were anesthetized with a mixture of etorphine hydrochloride and acepromazine maleate. Mean (± SD) IOP in the ibex was 17.95 ± 4.78 mmHg (24 eyes, indentation tonometry). In the zebra, indentation tonometry (20 eyes) yielded a mean IOP of 25.30 ± 3.06 mmHg, and applanation tonometry (six eyes) yielded a mean IOP of 29.47 ± 3.43 mmHg. In the oryx, indentation tonometry (five eyes) yielded a mean IOP of 22.68 ± 8.15 mmHg, and applanation tonometry (10 eyes) yielded a mean IOP of 11.76 ± 3.43 mmHg. There were no significant effects of gender, age, weight, side or reading number on the IOP measured in any of the three species. No significant differences were found between the IOP of the three species, nor between the readings of the two instruments, although some of the P -values were close to the significance level. 相似文献
4.
Ecology Habits of Equus hemionus and Gazella subgutturosa in Kalamaili Ungulate Reserve in Autumn 总被引:5,自引:0,他引:5
IntroductionKalamailiUngulateReserVe(88"30,-90o001E,44o46,-46o00'N),beingfoundedin1982andcovering17,000km',issituatedinsoutheasternDzungariaBasininXinjiang.Wildass(Equushemionus)andGoitredgazelle(GazedssUbgutturosa)arerankedrespectivelyasclass1and11proteCtedanimalsinChina11].ThereservebelongstoinlandlowhillydesertwitheXtremehotsumm\erandcoldwinter.Thevegetationfeaturepresentsscarcecoverage,dwarfplantsandlowbiomes.DominantplantsareHaIOXyIOnammodondfon,ReaumUfissoogaFI'Ca,Art6miSiBsp… 相似文献
5.
The food habits of Wild ass (Equus hemionus) and Goitred gazelle (Gazella subgutturosa) in kalamaili Ungulate Reserve in autumn was described, based on the investigation conducted in 1992. Wild ass was much concentrated
around water sources, and had more food resource than Goitred gezelle. The quagmires in the reserve were critical conditions
as food and water bases for both species. In terms of present survey, it was estimated that 680 Wild ass and 8840 Goitred
gazelle lived in this reserve.
(Responsible Editor: Zhu Hong) 相似文献
6.
The successful introduction of captive bred takhi or Przewalski’s horse, Equus ferus przewalskii, into Mongolia in the 1990s is a good example of the benefits of ex situ conservation and one of the few examples of the recovery of an animal after it became extinct in the wild. This is also particularly interesting because virtually nothing was known about how takhi lived before they died out, and the introductions have enabled us to study how they have settled, and their ecology and behaviour within their former natural range. In this paper, we describe the movement, home range size and shape, and habitat use of takhi at one of the release areas, the 570 km2 Hustai National Park in Mongolia. Harem home ranges varied between 129 and 2399 ha, with 80% core areas of between 61 and 1196 ha. There was no relationship between range size and harem size, or length of time since release. Initially, harems stayed near their release enclosures, but over time they established home ranges further away. There was little overlap between home ranges of different harems, but neither was there evidence of exclusive range use. The more nutritious vegetation at lower elevations was preferentially selected. Thus the present situation looks good, but, as the population continues to grow, we anticipate that there will be potential problems related to intraspecific competition for water and vegetation resources, and the potential for hybridisation with domestic horses belonging to the local people. We consider the time it may take for takhi to reach carrying capacity within Hustai National Park and emphasise that continual monitoring of the population is essential because interventional management is likely to be required in the future. 相似文献
7.
8.
采用聚丙烯酰胺凝胶电泳对藏野驴与家驴4种血清同工酶进行比较研究。结果发现,除LDH同工酶以外,藏野驴与家驴的酯酶、淀粉酶和碱性磷酸酶酶谱都有颇大的差异。 相似文献
9.
王旭东 《云南农业大学学报(自然科学版)》1991,6(2):116-121
本研究通过CIEP试验在血清中查到特异性循环抗体、免疫荧光和免疫过氧化物酶(IF和IP)染色检出肺组织存在多量IgG和S.T.孢子残留物以及ANAE染色结果表明:在S.T.的致病过程中存在Ⅲ和Ⅳ型变态反应。试验结果还暗示:无机尘有可能做为免疫佐剂而促进有机尘致病机理中的Ⅲ型变态反应。 相似文献
10.
The Polish Konik is a primitive breed of horse, most likely descended directly from the wild extinct forest equus so-called Tarpan (Equus ferus ferus). The studbook of this breed is closed, and only individuals with blue dun in color with a well-developed black middorsal stripe and other primitive markings are entered. The aim of the presented study was the investigation of genetic background of dun phenotype in Polish Konik horses. A total of 93 Polish Koniks were investigated toward single nucleotide polymorphisms in a region of TBX3 gene by Sanger sequencing method. Obtained results confirmed that all investigated horses carry dun dilution genotype. Two new variants were identified which have not been previously described [WT/WT-G/G-A/G; WT/del-G/del-G/G]. Furthermore, our results showed in population of Polish Koniks occurrence of the rare genotype recently found in Estonian-native horses. 相似文献