‘哈伯’南天竹组织培养和快速繁殖

为建立‘哈伯’南天竹组织培养和种苗繁育技术体系,以半木质化带芽茎段为外植体材料开展植株再生研究。通过观察对比试验法、L₉(3⁴)正交试验设计完全随机法、极差分析、显著性检验、LSD多重比较,探讨了‘哈伯’南天竹组培的最适培养基配方。试验结果表明：最佳诱导培养基为MS + 6-BA 2.0 mg/L + IBA 0.1 mg/L +蔗糖30 g/L,诱导萌动率71.77%,成活率85.51%;最佳增殖培养基为WPM +6-BA 1.5 mg/L + IBA 0.01 mg/L + 蔗糖30 g/L,增殖系数6.3;最佳生根培养基为1/2 MS+ IBA 0.5 mg/L + NAA 1.0 mg/L + 蔗糖20 g/L + AC 0.2 g/L,生根率97.63%;试管苗移入泥炭土:珍珠岩=3:2(V/V)混合基质中,移栽成活率96.67%。该试验建立了高效稳定的组培快繁技术体系,得到的组培苗后代能够稳定的保持母本优良性状,为工厂化育苗提供了技术支撑。     

杉木第3代种子园多元素配方施肥效应研究

杉木种子园为培育高产稳产杉木良种提供重要保障,在林业生产中具有积极意义。通过试验研究,探究杉木3代种子园土壤养分丰缺情况、养分间的平衡规律,确定杉木种子园最佳肥料配比和施肥量,以期为闽北低山丘陵红壤分布区杉木种子园提供推荐施肥配方。采用“3414”配方施肥设计方案,对闽北第3代杉木种子园开展N、P、K、Ca、Mg、B、Mo等元素施肥试验。分别设计大量元素氮、磷、钾三因素施肥水平（并增设施用钼肥处理）、中微量元素钙、镁、硼三因素施肥水平,以球果数量（质量）、出籽率、种子产量、胸径等为评价指标,拟合肥料效应函数方程,研究各养分间的作用规律,并提出最佳施肥配比和施肥量。结果表明：种子园土壤N、P、K、Ca、Mg养分含量较低,B含量属于中等水平,而Mo含量较高;N、P、Mg以及N-K、Ca-B肥料联合对种子园产量的影响达到显著或极显著水平,而单施某一肥料效应较小,其中单施K、Ca、B几乎没有效果,而肥料联合施用效果较好。综合效应分析结果,得出种子园最大产量施肥量组合为：每株施尿素100 g+过磷酸钙897 g+氯化钾150 g+石灰150 g+硫酸镁105 g+硼砂75 g,并配施钼肥。N、P₂O₅、K₂O、CaO、Mg、B、Mo的用量依次为46.0、107.7、90.0、75.0、31.4、2.3、5.0 g,其配比为N:P₂O₅:K₂O:CaO:Mg:B:Mo=1.0:2.3:2.0:1.6:0.7:0.05:0.1。多元素配方施肥能很好地改善杉木种子园产量和质量,单株球果产量、球果单粒重、出籽率及种子园产量等指标均比不施肥的母树有大幅度提高。本研究施肥方案,理论上种子园产量可达45.99~95.65 g/株,大量元素施肥试验的产量达到理论产量的80%~86%,中微量元素施肥试验的产量为理论产量的70%左右。现实种子园平均产量大约是预测产量的40%,通过合理配方施肥有望大幅度提高杉木3代种子园产量。     

鸡的微卫星DNA标记与胴体性状的相关分析

测定了青脚麻鸡与隐性白鸡杂交后自交的F2代个体的全净堂重、全净堂率、脂肪宽带、腹脂重和腹脂率6个胴体性状,检测10个微卫星DNA标记,对标记基因型胴体性状值进行方差分析和多重比较,结果表明:MCW328对于10周龄体重和全净膛重两性状各基因型均有显著的差异(P<0.05);ADL292对于腹脂重性状各基因型有极显著的差异(P<0.01),对于腹脂率有显著的差异(P<0.05)。MCW328标记基因与控制10周龄体重和全净膛重QTL连锁,ADL292标记基因与控制腹脂重和腹脂率QTL连锁。     

高寒围栏草地短期放牧育肥牦牛增重试验

选择 40头成年阉牦牛 ,( 2 0头为试验组、2 0头为对照组 ) ,进行围栏草地上短期放牧育肥试验。结果 ,在围栏草地上放牧育肥的试验组阉牦牛 ,试验组每头牦牛的总增重比对照组提高 3 .3 1kg ,平均日增重 83g ,提高 44 % ,(P <0 .0 1)。试验组比对照组牦牛每头分别增收 2 6.48元     

对江苏省发展木片生产的几点思考

论述了江苏发展木片生产的意义和现状，分析了存在的问题及有利条件，提出了８条切实可行的建议。     

N_(98s)×特青(亚种间杂交稻)抽穗后物质生产及分配特点研究初报

对N×特青示范田中试区与肥料试验田各处理小区稻株分析结果表明:抽穗后,亚种间杂交稻N×特青比常规稻特青干物质生产能力强,灌浆速度快;地上部各器官占植株干重比例,前者叶片比重大于茎,后者茎比重大于叶。亚种间杂交稻对肥料反应敏感,抽穗后,干物质累积,植株高度,每穗总粒数和粒重均随施肥量增加而增加,在高肥区有利于进一步提高产量,但增施氮肥后,灌浆速度明显滞后。     

生沛素对皖系粗毛兔生产性能的影响

选择42日龄皖系粗毛兔幼兔42只,根据体重、性别,随机分成3组,分别饲喂含1‰、2‰生沛素日粮和对照组日粮.结果表明,添加1‰、2‰生沛素组产毛量比对照组分别提高17.5%(P＜0.01)和19.1%(P＜0.01);增重提高11.2%(P＜0.05)和12.5%(P＜0.05);每只兔一次产毛的经济效益比对照组分别提高2.96元和1.13元.另外,添加生沛素的试验组兔毛品质也比对照组有所提高和改善.     

西杂牛连续埋植“畜大壮”效果观察

本试验对甘肃河西地区西杂１－２岁和周岁以下的犊牛连续用促生长剂“畜大壮”分阶段进行耳根埋植,试验结果表明,４－６月龄犊牛全期１８０天,试验组增重和平均日增重分别为５．５ｋｇ和３０８．３３ｇ,试验组比对照组提高３７．２５ｋｇ和２０６．９４ｇ（Ｐ〈０．０１）,１－２岁牛全期１８０天,试验组平均增重和日增重分别为５６．４３ｋｇ和３１４．４９ｇ,试验缄比对照组分别提高３０．０３ｋｇ和１６６．８２ｇ差异显著     

Estimation of daily and total lactation milk yield of Chios ewes from single morning or evening records

Linear models were developed and evaluated for the estimation of daily (24 h) and total lactation milk yield of dairy sheep from single morning (am) or evening (pm) milking records. A total of 22,908 individual test-day milk records of 5110 ewes of the Chios breed, raised in 46 flocks, were used. Depending on the model, different daily milk yield estimates were derived for each ewe, accounting for lactation number, stage of lactation, season of previous lambing and interval between successive milkings. Daily milk yield was also estimated from doubling the single am or pm record. Actual and estimated yields were compared using root mean square errors (RMSEs), mean absolute differences, an accuracy parameter defined as the ratio of the actual yield variance over the sum of the variances of actual yield and absolute difference, and the product moment correlation between estimated and actual yield. Results were validated on independent datasets. Linear models resulted in less biased and more accurate estimates of the daily milk yield than simply doubling the am or pm record. Root mean square errors decreased by 7-37% and the mean absolute difference was reduced by 1-4% of the average daily yield. Higher accuracy and correlation were obtained from linear model application than doubling. Total lactation milk yield was predicted based on actual daily yield and compared to predictions based on estimated daily yield from am or pm records, as well as directly on single milking records. Root mean square errors obtained when daily yield had been estimated with linear models were 26-35% lower compared to doubling the am or pm yield and 0-13% lower compared to estimating the total lactation yield directly from single milking records. Linear model application also resulted in lower mean absolute difference and higher accuracy and correlation than doubling the am or pm record. Recording the yield of a single milking (am or pm) instead of both can benefit milk recording by reducing its cost and increasing farmer participation. In this context, linear models developed in the present study can be used for the accurate estimation of daily (24 h) and total lactation milk yield from single milking records.     

香菇出口生产技术综述

香菇无公害生产全程技术监控，包括优化产地生态环境栽培原材料把关，精选优良菌种，菌袋生产规范化，出菇科学管理，控制农药使用，产品标准化等七个方面。