抗TY番茄新品种海纳178的选育

海纳178是以K-1-4-5-1-3为母本,H-5-1-4-2-2为父本组配而成的番茄一代杂种。该品种为无限生长类型,植株生长健壮;成熟果大红色,色泽鲜亮;果硬、耐贮运,单果质量180 g左右,中熟,抗黄化曲叶病毒病,植株综合抗逆性强。     

昆虫病原格氏线虫与小卷蛾线虫染色体组型的研究

以格氏线虫(NC513)及小卷蛾线虫(DD-136)的幼胚、精巢及卵巢为材料,用地衣红染色、压片法制作玻片标本、对其染色体组型进行了研究,结果表明,两种线虫的染色体数目均为雌性2 N=10,雄性2 N=9。但它们的染色体组型并不一致,两种线虫的染色体组型可概括为以下公式。格氏线虫(NC513):2N=4m+6t(雌),2N=4m+5t(雄)。小卷蛾线虫(DD-136):2N=8m+2t(雌),2N=7m+2t(雄)。     

适宜麦套的晚春播棉花品种冀178选育研究

冀178是河北省农林科学院棉花研究所以选自夏播短季棉品种中棉所16的508系为母本、以综合性状优良的169系为父本选育而成的适宜棉麦套作的晚春播棉花新品种,2015年通过河北省农作物品种审定委员会审定(审定编号:冀审棉2015010号)。该品种具有早熟、稳产、纤维品质优良、抗病性好、适宜棉麦套作等特点,在棉麦套作模式下棉花霜前花率可达87.2%且显著高于其他参试品种,对促进棉田增效和加快河北省棉花种植结构调整具有重要意义。     

胶孢炭疽菌致病相关基因Plv2功能分析

胶孢炭疽菌(Colletotrichum gloeosporioides)引起的橡胶树炭疽病是橡胶树三大叶部病害之一,严重威胁着橡胶树的生长。本研究从构建的胶孢炭疽菌RC178 T-DNA突变体库中筛选获得一株致病力明显减弱的突变菌株T1103,其T-DNA为单拷贝。采用TAIL-PCR克隆了该突变体T-DNA插入位点的侧翼序列,通过比对胶孢炭疽菌全基因组序列,发现该TDNA插入位点所在的序列包含一个5 400 bp的基因,将其命名为Plv2。Plv2基因包含3个外显子,编码一个假定的甾醇C-24甲基转移酶。敲除野生型菌株RC178中的Plv2,发现敲除突变体与T1103的致病力表现基本一致,由此推测Plv2基因为致病相关基因。     

The genetic architecture of zinc and iron content in maize grains as revealed by QTL mapping and meta-analysis

Micronutrient malnutrition, especially zinc (Zn) and iron (Fe) deficiency in diets, has aroused worldwide attention. Biofortification of food crops has been considered as a promising approach for alleviating this deficiency. Quantitative trait locus (QTL) analysis was performed to dissect the genetic mechanism of Zn and Fe content in maize grains using a total of 218 F_2:3 families derived from a cross between inbred lines 178 and P53. Meta-analysis was used to integrate genetic maps and detect Meta-QTL (MQTL) across several independent QTL researches for traits related to Zn or Fe content. Five significant QTLs and 10 MQTLs were detected. Two informative genomic regions, bins 2.07 and 2.08, showed a great importance for Zn and Fe content QTLs. The correlation between Zn and Fe level in maize grains was proposed by MQTLs as 8 of the 10 involved both traits. The results of this study suggest that QTL mapping and meta-analysis is an effective approach to understand the genetic basis of Zn and Fe accumulation in maize grains.