基于机器视觉的棉种破损检测技术

研究了破损棉种的机器视觉识别方法,采用均值、方差、均方比等统计特性参数,计算棉种边界破损参数.通过实验确定均方比分类阈值为0.58,将棉种分为破损棉种和正常棉种.选取正常棉种330粒、破损棉种110粒,利用该检测系统进行检测,其识别精度达93%.     

Identification of resistance to Aspergillus flavus infection in cotton germplasm

Natural resistance in cottonseed to Aspergillus flavus infection has not been explored to date. A green fluorescent protein (GFP) expressing A. flavus strain was used to assess the resistance of seed from 35 cotton varieties from Gossypium arboreum, G. barbadense, and G. hirsutum. Mature cotyledons devoid of seed coat were wounded, inoculated, and assessed for innate resistance to A. flavus infection. Of the initial 35 varieties tested, we observed a range of resistance to infection in representatives of all three species. A subset of 15 representatives was further analyzed. Within this group, G. arboreum cultivar A2 186 and G. hirsutum cultivar SA 1582 were most resistant to fungal infection. The most susceptible cultivar in this group was G. hirsutum SA 1595. The remaining 12 representatives tested in the secondary screen (3 G. arboreum, 3 G. barbadense, and 6 G. hirsutum lines) exhibited intermediate resistance. We did not observe any relationship between species and resistance. Within each species, there was a range of responses to fungal infection. Future studies using this methodology to screen additional diploid and tetraploid cotton lines may enable us to identify naturally resistant germplasm that can be used to develop cotton with enhanced resistance to fungal infection.     

Effects of dietary cottonseed meal protein level on growth, gonad development and plasma sex steroid hormones of tropical fish tilapia Oreochromis sp

Five experimental diets containing increasing proportions of cottonseedmeal (CSM) protein (0, 25, 50, 75 and 100%; diets 1 to 5, respectively) toreplace fish meal (FM) protein were formulated for intensive culture of tilapiaOreochromis sp. Each diet was fed to three replicategroupsof fish (mean weight ± SE = 11.3 ± 3.9 g) in30L aquaria connected as a closed recirculating-water system andmaintained at 27 ± 1 °C. Fish were fed three times adayby hand at a rate of 3% of body weight during four weeks, after which thefeeding rate was gradually decreased to reach 1.5% at 16 weeks. Thesubstitutionof 75 and 100% of FM proteins by CSM proteins resulted in significantly lowerbody weights in both sexes. In both sexes, gonadosomatic indexes and plasmaconcentrations of sex steroids (testosterone, 11-ketotestoterone,estradiol-17 and 17,20-dihydroxy-4-pregnen-3-one) were notsignificantly different among dietary treatments. The concentration ofgossypol,an antifertility agent contained in CSM, was measured in reproductive tissues.The total gossypol concentration in the testis was consistently lower than thatmeasured in the ovaries of the same group. Moreover, in both sexes, theconcentration of the (+)isomer of gossypol was always higher than that of(–)isomer. The total gossypol concentrations in testes increasedsignificantly with the increase of CSM in the diet. The highest levels of the(+)isomer (7.64 ± 0.62 g g^–1)were found in the testes of fish fed diet 4, whereas the (–)isomerreached its highest values in the testes of fish fed diet 5. The highest levelsof both enantiomers of gossypol were found in the ovaries of fish fed diet 4(14.2 ± 2.7 and 5.6 ± 1.5 g g^–1for (+) and (–)isomers, respectively). In both sexes, thehistological analysis of the gonads did not reveal differences among the fishfed different levels of CSM. Although CSM at any levels did not affect thereproductive parameters examined in this study, it cannot be used to substitutemore than 50% of FM since at higher levels growth of tilapia was compromised.     

秸秆还田替代化学钾肥对棉麦轮作中棉仁油分累积的效应

为研究棉田化学钾肥的秸秆替代施入对棉仁含油量的影响及其生理生化基础,2012—2013年于江苏省农业科学院试验站进行麦棉两熟周年秸秆还田定位试验,在棉花季设置小麦秸秆不还田(0,W0)、半量还田(4500 kg hm–2,W1)和全量还田(9000 kg hm–2,W2),在小麦季设置棉花秸秆不还田(0,C0)、半量还田(3750 kg hm–2,C1)和全量还田(7500 kg hm–2,C2),两种作物秸秆不同还田量组合后共9个秸秆还田处理,另根据秸秆折合钾肥量,于2012年棉花季开始增设2个钾肥用量处理,即150 kg K2O hm–2和300 kg K2O hm–2(K1和K2)。研究显示,在适宜氮肥(300 kg N hm–2)、磷肥(150 kg P2O5 hm–2)水平下,随着逐年秸秆还田与施钾,土壤速效氮、有效磷年际间差异均不显著,但土壤速效钾含量年际间存在显著差异;花后17 d、24 d是不同处理条件下棉仁含油量差异形成的关键时期;相较于6-磷酸葡萄糖脱氢酶(G6PDH)、磷酸烯醇式丙酮酸羧化酶(PEPC),花后17 d、24 d的磷脂酸磷酸酯酶(PPase)的活性对棉仁油分的通径系数更大。结果表明,短期秸秆还田与单施化学钾肥均主要影响棉田土壤速效钾含量;从棉仁油分累积角度来看,秸秆还田可在很大程度上替代化学钾的施入;花后17 d、24 d棉仁钾含量是影响棉仁含油量的关键因子;秸秆还田替代化学钾肥条件下,PPase较G6PDH、PEPC对棉仁油分的影响更为关键。     

温,湿度条件及不同种子处理对棉籽吸胀冷害的影响

棉籽在4－5℃条件下吸水，发生吸胀冷害的程度随吸水速度的加快而减轻，随吸水时间的延长而加重；在10-12℃、18－20℃的水中吸水分别超过16小时、12小时，会因吸水时间过长使部分棉籽的种子活力降低，而使正常发芽率降低。经温汤浸种和预冷处理的棉籽能耐72小时0－5℃的低温，且耐较长时间低温的能力强于干籽与晾干籽；干籽、晾干籽能耐24小时0－2℃的低温，且干籽耐短时间低温的能力强于湿籽。     

棉子品质性状的遗传研究

本研究采用6个陆地棉亲本的Griffing双列杂交设计模式Ⅱ对5个棉子品质性状进行了Hayman双列遗传模型分析.结果表明,种子蛋白质和油分百分含量、种子蛋白质和油分指数及仁指都适合“加性—显性”模型.种子蛋白质百分含量和指数以显性效应为主且表现超显性;种子油分百分含量和指数以加性效性应为主且表现部分显性;仁指加性和显性效应都较重要,接近完全显性.在所研究的亲本中,不同性状的显、隐性基因频率,正负效应基因的比例,显、隐性基因的方向及狭义遗传力等都明显不同.     

卡拉库尔羊血液生化指标常值测定

从大群卡拉库尔羊(以下简称卡羊)中选择公羊羔18只，自由采食中棉35号大陆棉棉壳饲料，利用半自动生化分析仪测定血液生化指标。结果表明：与绵羊临床正常生化指标相比，卡羊的总蛋白、碱性磷酸酶测定值偏高；白蛋白、低密度脂蛋白胆固醇、谷丙转氨酶测定值偏低；其它指标都在正常值范围内。     

饲喂棉籽饼水牛尿石症发生中血清维生素A含量变化及其作用的研究

选用１２头无既往病史，９－１２月龄末去势公水牛随机分为４组，每组３头。采用饲喂棉籽饼与经加水煮沸１小时后的棉籽饼，添加与不添加维生素Ａ的方法，进行了１２周的人工诱病和预防试验。对参试水牛血清中维生素Ａ含量的动态变化进行了观察。结果表明：饲喂棉籽饼与饲喂经加水煮沸的棉籽饼组间，添加与不添加维生素Ａ组间，表现与不表现临床尿石症水牛间血清维生素Ａ含量差异不显著（Ｐ＞０．０５），试验期间各组水牛血清维生素     

Partial replacement of fish meal by cottonseed meal and soybean meal with iron and phytase supplementation for parrot fish Oplegnathus fasciatus

Two consecutive feeding trials were conducted to determine the optimum dietary level of the cottonseed and soybean meal (CS) for replacement of fish meal (FM) in diets for juvenile and ongrowing parrot fish with or without iron and phytase. In experiment I, juvenile parrot fish (BW: 3.17 g) were fed one of six experimental diets for 12 weeks which were formulated to replace FM protein by equal proportion (1:1, w:w) of CS at 0, 10, 20, 30, 40, or 50% (designated as CS0, CS10, CS20, CS30, CS40, or CS50, respectively). In experiment II, ongrowing parrot fish (BW: 55 g) were fed one of five experimental diets for 9 weeks. The experimental diets were formulated to replace FM protein by CS at 0, 20, or 30% (designated as CS0, CS20, or CS30, respectively) and to include ferrous sulfate (0.1 and 0.2%) and phytase into the CS20 and CS30 diets (CS20 + Fe&P and CS30 +Fe&P, respectively). Results from the two feeding trials indicated that the CS could replace up to 20% FM protein in diets for juvenile parrot fish (3–22 g) and up to 30% in ongrowing (55–120 g) parrot fish. The supplemental effect of iron and phytase was not significant on growth performance. Total or each gossypol enantiomer concentration in the liver increased as the level of dietary cottonseed meal increased. However, gossypol in the liver of fish fed diets supplemented with iron was not detected. The dietary supplementation of CS significantly reduced the levels of plasma triacyglycerols and total cholesterol. The present study indicates that plant protein sources are better utilized in larger fish, and that up to approximately 30% FM protein could be replaced by CS with iron and phytase in the presence of supplemental lysine and methionine.     

Lipids and Tocopherols in Serum and Liver of Female Rats Fed Diets Containing Corn Oil or Cottonseed Oil

Growing female rats were fed diets containing either corn oil (CO) or cottonseed oil (CSO) to determine if the previously reported lowering effect of CSO versus CO on serum total cholesterol (TC) and high-density lipoprotein cholesterol (HDL-C) was sex specific and to compare the effect of these two oils on serum and tissue concentrations of RRR-alpha-tocopherol (α-T) and RRR-gamma-tocopherol (γ-T), the two major tocopherols in these oils. In a 4-week study, groups of rats (n=10 each) were fed diets containing 100 g/kg of either CO or CSO. TC was lower for group CSO than group CO. Serum concentrations of HDL-C, non-HDL-C, triglycerides (TGs), as well as the TC/ HDL-C ratio and the hepatic concentrations of cholesterol and TGs, were unaffected by diet. For serum and liver, between-group differences were noted for the concentrations of α-T (where values were higher for group CSO) and γ-T (where values were lower for group CSO). These differences reflected differences between the oils in their concentrations of these tocopherols. Thus, CSO has a lowering effect on TC for both sexes, but on HDL-C for male animals only; replacement of CO with CSO results in changes in tocopherol status.