多花黑麦草品种比较试验

通过对现行生产中常用的4个多花黑麦草品种在昆明地区的物候期、株高、草产量、生长率、净同化率等指标的规定表明,在生长初期,特高的生产性能比其余3个品种都好,但生长一段时间后,邦德的生产性能明显超过特高(P<0.05),抗锈王略高于特高(P>0.05),旺饲的生产性能最差(P<0.05)。因此,邦德是一个在生产中值得推广应用的品种。     

Computed Tomographic Angiography of the Pancreas in Cats with Chronic Diabetes Mellitus Compared to Normal Cats

Background

Diabetes mellitus (DM) is a common endocrinopathy in cats. No known diagnostic test or patient characteristic at the time of diagnosis can predict likely disease course, unlike in people in whom computed tomographic angiography (CTA) is used. No published data exist regarding the CTA appearance of the pancreas in cats with DM, and thus, it is unknown what if any CTA variables should be further assessed for associations with pancreatic endocrine function.

Hypothesis/Objectives

A significant difference in pancreatic attenuation, volume, and size will be identified between normal cats and those with chronic DM on CTA.

Animals

Ten healthy control cats and 15 cats with naturally occurring DM present for >12 months.

Methods

Prospective cross‐sectional study comparing pancreatic attenuation, enhancement pattern, size, volume, pancreatic volume‐to‐body weight ratio (V:BW), pancreatic arterial: portal phase ratio (A:P), time‐to‐arterial enhancement, and time‐to‐peak portal enhancement on CTA between sedated healthy control cats and those with chronic DM.

Results

The pancreas in cats with chronic DM was significantly larger, had higher volume, higher V:BW, and shorter time‐to‐peak portal enhancement on CTA when compared to normal cats.

Conclusions and Clinical Importance

Peak portal enhancement time, pancreatic size, pancreatic volume, and V:BW can be used to differentiate normal sedated cats from those with chronic DM by CTA. These variables warrant further investigation to identify possible associations with endocrine function.     

西丰杨6号地膜覆盖扦插对比试验

通过对西丰杨6号地膜扦插试验,西丰杨具有生长快,成活率高,抗病性强等特点。是植树造林的优良树种。     

西藏仿野生种植天麻与野生天麻中天麻素含量的对比分析

[目的]分析西藏仿野生种植天麻Gastrodiaelata中天麻素含量,并将其与野生天麻中天麻素的含量进行对比。[方法]采用HPLC测定天麻素含量。[结果]西藏波密县仿野生种植天麻的天麻素介于0.41～2.28 g/kg,其平均含量为0.88 g/kg,比当地野生天麻高4倍。[结论]在生长的生态环境、生长年限、采挖季节相同或较接近情况下,仿野生种植天麻品质高于当地野生天麻。在波密县推广仿野生种植天麻技术切实可行,该项目有较强的经济价值。     

提高日本南瓜坐果率的栽培措施

通过对比试验,研究了提高日本南瓜坐果率的栽培管理方法。     

2种降水模式对2次降水过程的对比分析

利用T639模式与日本模式的降水数值预报,对2008年7月的2次降水天气过程进行对比分析。结果发现,日本模式与T639模式的36和60h实效预报情况较好,降水落区和降水强度基本可以信任。2008年7月6日过程T639模式和日本模式东北地区的36h时效日累积降水预报除对暴雨区的位置有偏差外,降水带的落区和量级与实况较为一致;2008年7月24日过程的36和60h实效的预报效果与实况大体一致。但从2次过程的84h预报情况来看,降水落区和降水强度均有较大偏差。     

Involvement of TLR4/NLRP3 inflammasome in contrast medium-induced inflammation and injury in renal tubular epithelial cells

AIM: To investigate whether Toll-like receptor 4 (TLR4) and Nod-like receptor protein 3 (NLRP3) inflammasome were involved in contrast medium (CM)-induced inflammation and injury in renal tubular epithelial cells. METHODS: Iopromide was used to injure NRK-52E cells in the study. The cell viability was measured by CCK-8 assay. The protein levels of TLR4, NLRP3, apoptosis-associated speckle-like protein (ASC), caspase-1 and cleaved caspase-3 were determined by Western blot. The releases of interleukin (IL)-1β and IL-18 were detected by ELISA. The apoptotic rate was evaluated by Hoechst staining, and mitochondrial membrane potential (MMP) was analyzed by JC-1 staining. siRNA was transfected into the NRK-52E cells to silence NLRP3 expression. RESULTS: CM decreased the viability of NRK-52E cells (P<0.05). CM also elevated the protein levels of cleaved caspase-3, TLR4, NLRP3, IL-1β and IL-18 (P<0.05). Silencing NLRP3 attenuated CM-induced releases of inflammatory cytokines. Moreover, treatment with TLR4 inhibitor TAK-242 or knockdown of NLRP3 by siRNA transfection both attenuated cell apoptosis and loss of MMP caused by CM. CONCLUSION: TLR4/NLRP3 inflammasome takes part in the pathogenesis of CM-induced acute kidney injury, and mediates CM-induced injury and inflammation in renal tubular epithelial cells.     

转基因玉米转化体特异性PCR检测技术研究

根据不同转基因玉米中重组DNA结构,分别对转基因玉米Bt11、Bt176、Mon810、Mon863、TC1507、GA21和NK603设计转化体特异性引物进行PCR检测。在此基础上分别以玉米内参照基因(ZSSIIb)作对照,对Bt11、Mon810、TC1507和GA21、NK603、Bt176分别作两对四重PCR反应,实现在同一个PCR反应管中同时对3种转基因玉米及内参照基因的特异性检测。     

EM发酵玉米秸秆和青贮玉米秸秆对奶牛生产性能的对比试验

本试验应用EM发酵玉米秸秆和青贮玉米秸秆饲喂奶牛,以观察两种方法处理的玉米秸秆对奶牛生产性能及经济效益的影响效果。结果表明,用EM发酵的玉米秸秆饲喂奶牛较青贮玉米秸秆饲喂奶牛,每天每头奶牛采食量提高1.03%,平均产奶量提高2.02%,经济效益提高0.13元;EM原液发酵的玉米秸秆和青贮玉米秸秆1∶1混合饲喂奶牛,每天每头奶牛采食量、平均产奶量、经济效益分别比饲喂青贮玉米秸秆的奶牛提高2.85%、2.99%和1.15元。     

Use of Contrast‐Enhanced Fluid‐Attenuated Inversion Recovery Sequence to Detect Brain Lesions in Dogs and Cats

Background

The diagnostic value of a contrast‐enhanced T2‐weighted FLAIR sequence (ceFLAIR) in brain imaging is unclear.

Hypothesis/Objectives

That the number of brain lesions detected with ceFLAIR would be no greater than the sum of lesions detected with nFLAIR and ceT1W sequence.

Animals

One hundred and twenty‐nine animals (108 dogs and 21 cats) undergoing magnetic resonance imaging (MRI) of the head between July 2010 and October 2011 were included in the study.

Methods

A transverse ceFLAIR was added to a standard brain MRI protocol. Presence and number of lesions were determined based on all available MRI sequences by 3 examiners in consensus and lesion visibility was evaluated for nFLAIR, ceFLAIR, and ceT1W sequences.

Results

Eighty‐three lesions (58 intra‐axial and 25 extra‐axial) were identified in 51 patients. Five lesions were detected with nFLAIR alone, 2 with ceT1W alone, and 1 with ceFLAIR alone. Significantly higher numbers of lesions were detected using ceFLAIR than nFLAIR (76 versus 67 lesions; P = 0.04), in particular for lesions also detected with ceT1W images (53 versus 40; P =.01). There was no significant difference between the number of lesions detected with combined nFLAIR and ceT1W sequences compared to those detected with ceFLAIR (82 versus 76; P =.25).

Conclusion and Clinical Importance

Use of ceFLAIR as a complementary sequence to nFLAIR and ceT1W sequences did not improve the detection of brain lesions and cannot be recommended as part of a routine brain MRI protocol in dogs and cats with suspected brain lesions.