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S. Haneda K. Nagaoka Y. Nambo M. Kikuchi Y. Nakano M. Matsui Y. Miyake J.N. Macleod K. Imakawa 《Domestic animal endocrinology》2009,36(4):209-218
To identify factors involved in the establishment of pregnancy in the mare, endometrium was collected from day 13 (day 0 = day of ovulation) cyclic and day 13, 19, and 25 pregnant animals. From initial cDNA subtraction studies, interleukin-1 receptor antagonist (IL-1RN) mRNA was found as a candidate molecule expressed uniquely in the pregnant endometrium. Expression of IL-1RN mRNA was markedly increased in day 19 and 25 gravid endometrium. In situ hybridization analysis revealed that IL-1RN mRNA was localized to the glandular epithelium. Interleukin-1 receptor antagonist (IL-1RN) protein was found in the extracts of day 25 gravid endometrium and was immunochemically localized to the glandular epithelium/luminal cavity of the pregnant uterus. High concentrations of estradiol-17β (E2) were detected in day 25 conceptuses. Concentrations of E2 were higher in the gravid endometrial portion than in other endometrial regions. On the other hand, progesterone concentrations did not differ among endometrial samples analyzed. Furthermore, the expression of IL-1RN mRNA was up-regulated in endometrium culture samples treated with 10 ng/mL E2 and 10 ng/mL progesterone. In the analysis of related gene expression, increased amounts of IL-1α and IL-6 mRNA were also found in the day 25 gravid endometrium; however, these expressions in endometrial culture samples were not up-regulated by the steroid treatment. These results indicate that expression of IL-1RN in the endometrium is likely regulated by E2 and progesterone and suggest that IL-1RN regulates the degree of IL-1 signal transduction and thereby plays an important role in the establishment of equine pregnancy. 相似文献
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The objectives of the present study were to establish a nonsurgical transfer method for elongating bovine conceptuses and to
combine this method with biopsy and sexing. Bovine conceptuses were recovered from donor cows on days 13–14 of the estrus
cycle. In experiment 1, day 13 conceptuses were transferred to recipient cows using a standard day 7 embryo transfer (ET)
method. The pregnancy rate of day 13 conceptus transfer (CT) is comparable to that of day 7 ET. In experiment 2, day 14
conceptuses were transferred using modified methods (balloon catheters or ET guns with modified sheaths). Using the standard
ET method, no pregnancies were obtained; however, when balloon catheters or ET guns with modified sheaths were used, the
pregnancy rates after CT were 48.0% and 44.8%, respectively. In experiment 3, day 14 conceptuses were biopsied without a
micromanipulator, sexed using the loop-mediated isothermal amplification method and transferred to recipient cows. The
pregnancy rate of biopsied conceptuses was 46.2% and did not differ significantly from that of unbiopsied conceptuses.
Moreover, all pregnant cows transferred conceptuses following biopsy and sexing delivered calves with the expected sexes.
These results suggested that the nonsurgical bovine CT method was comparable to day 7 ET and that this technique enables
biopsy and sexing without expensive equipment such as a micromanipulator or specialized skills. 相似文献
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Agnieszka BLITEK Ewa MORAWSKA-PUCINSKA Magdalena SZYMANSKA Jolanta KIEWISZ Agnieszka WACLAWIK 《The Journal of reproduction and development》2013,59(6):512-519
Transforming growth factor (TGF) β and its receptors are expressed at the
conceptus-maternal interface during early pregnancy in the pig. The present studies
were conducted to examine: (1) the effect of conceptus products on TGFβ1 mRNA
expression and protein concentration in the porcine endometrium using in
vivo and in vitro models, and (2) the effect of TGFβ1 on
proliferation of porcine trophoblast cells in vitro. During
in vivo experiments, gilts with one surgically detached uterine
horn were slaughtered on days 11 or 14 of the estrous cycle and pregnancy. For
in vitro studies, endometrial explants and luminal epithelial
(LE) cells co-cultured with stromal (ST) cells were treated with conceptus-exposed
medium (CEM). Moreover, porcine trophoblast cells were treated with TGFβ1, and the
number of viable cells was measured. On day 11, the presence of conceptuses had no
effect on TGFβ1 mRNA expression, but decreased the TGFβ1 protein concentration in the
connected uterine horn compared with the detached uterine horn. In contrast to day
11, on day 14 after estrus, TGFβ1 mRNA expression and protein content in the
endometrium collected from the gravid uterine horn were greater when compared with
the contralateral uterine horn. The treatment of endometrial slices with CEM resulted
in greater TGFβ1 mRNA expression and protein secretion. LE cells responded to CEM
with an increased TGFβ1 mRNA level. Moreover, TGFβ1 stimulated the proliferation of
day 14 trophoblast cells. In summary, porcine conceptuses may regulate TGFβ1
synthesis in the endometrium at the time of implantation. TGFβ1, in turn, may promote
conceptus development by increasing the proliferation of trophoblast cells. 相似文献
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