比格犬人工感染埃立克体病的临床研究

为了表述比格犬感染埃立克体（Ehrlichia)后的临床表现，用已确证为Ｅ．platys和Ｅ．canis混合感染的犬血对５条比格犬进行了人工感染，并对临床和血液学变化进行了观察。埃立克体病的潜伏期为６－２９天，其后的临床表现为急性发热、眼结膜苍白、厌食、精神沉郁、体重严重减轻、腹泻带血、多处有淤血斑点出现、６－４５天内全部死亡。血液学变化显示血红蛋白和白细胞明显减少（Ｐ＜０．０１，Ｐ＜０．０５）。由此可见，比格犬对２种埃立克体（Ｅ.canis和Ｅ．platys）混合感染的性很高，严重的临床表现与美国学者普遍认为的亚临床状态相左，这可能是中国Ｇzh981株（Ｅ．platys)和Ｇzh982株（Ｅ．casnis)与国外株在毒力上的差异所致。     

直干桉、蓝桉无性系多点测定的初步分析

经方差分析后发现，３个试验点的直干桉、蓝桉无性测定林在１９９４年７月以前造的，其处理间的生长差异显著或极显著，直干桉的生长差异比蓝桉更大。将造林时的苗高作协方差分析，用回归系数调整树高后，大部分无性系的树高都超过对照。对无性系与环境的交互作用初步结果的分析表明，多数地点、地点ｘ无性系的差异显著。以实测值（并非调整值）为难，选出了材积超过对照２５％的直干桉无性系７个，蓝桉无性系６个，最好无性系的材积分别为对照的２８８％和２５６％。     

纵带球须剌蛾的生物学特性及其防治

纵带球须刺蛾是多种农林作物的害虫。在广州1年发生1～3代,以老熟幼虫在土中茧内越冬,幼虫一般为7～8龄,幼虫感染核多角体病毒后,常成为流行病,死亡率可达90%以上。进行防治试验表明,90%敌百虫晶体40000倍液、80%敌敌畏乳剂100000倍液和50%杀螟松乳剂100000倍液等,以及2.3×10~(5-7)浓度的核多角体病毒,均对幼虫有较好的毒杀效果。     

Effects of storage time and freezing temperature on clinical chemical parameters from canine serum and heparinized plasma

To assess changes in 24 blood constituents in frozen serum and heparinized plasma, blood samples were drawn from 10 clinically normal German Shepherd army dogs. The storage characteristics of nine enzymes (ALP, ALT, amylase, AST, CK, GGT, GLDH, LDH, lipase), and 15 metabolites and minerals (albumin, bile acids, bilirubin, calcium, cholesterol, creatinine, fructosamine, glucose, magnesium, phosphate, potassium, protein, sodium, triglycerides, urea) were studied. Parallel samples of serum and heparinized plasma were stored for 90 and 240 days at two different storage temperatures, -200 degrees C and -700 degrees C. Sixteen of the 24 analytes (ALP, ALT, amylase, AST, CK, GGT, GLDH, LDH, bile acids, calcium, cholesterol, creatinine, fructosamine, magnesium, phosphate, urea) showed statistically significant (p < 0.05) changes during the storage period related to storage time, storage temperature, and sample type. Seven of the analytes (amylase, GGT, GLDH, LDH, bile acids, fructosamine, magnesium) showed changes of possible clinical importance with mean differences from baseline larger than 20% for the enzymes and 10% for the metabolites and minerals during the storage periods.     

The application of atomic force microscope in clinic diagnosis of erythrocytes

AIM: To investigate the application of atomic force microscope (AFM) in clinic diagnosis. METHODS: Topographic images and some parameters of large range field and microstructures of erythrocytes in the blood of normal subjects, lung cancer and myelodisplastic syndrome (MDS) patients were examined by atomic force microscope. RESULTS: Many clear topographic images of many erythrocytes, single erythrocyte, and microstructure of erythrocyte membrane surface were obtained. Many erythrocytes in lung cancer patients were found to change into echinocytes. One erythrocyte had 10-20 protuberances, most of which, with a mean width of 589. 0 nm and a length of 646. 7 nm, were on the edge of cells. The protuberances on the center of echinocytes are lodged and embedded. The erythrocytes of MDS patients were biconcave in shape. Many apertures with different diameters of tens to hundreds nanometer appeared on the surface of cell membrane. CONCLUSIONS: AFM can be widely applied in clinic pathological inspection, including quantification of cells, obtainment and comparison of many parameters (such as diameter, thickness, volume, surface, surface area/volume ratio), observation of topograph of single cell, and observation and comparison of membrane surface microstructure of cells, and so on.     

留守村民的健康分析——基于株洲市茶陵县马加村的调查

采用调查问卷和临床问诊的方法对湖南省株洲市马加村随机抽样的252名留守村民进行调查,共发放调查问卷264份,回收问卷252份,回收率95.5%。调查表明:81.7%的被调查者有不同程度的身体疾病,其中血压偏高者57.3%,长期肌肉疼痛者69.4%,关节、骨异常者52.9%,腰间盘突出者16%。在此基础上,对影响留守村民的健康的因素进行了分析。     

不同药物防治羔羊奶结症临床效果分析

为寻找羔羊奶结症的有效防治药物，将筛选出的5组药物用于羔羊奶结症的防治试验？结果表明，第l组药物“消维康”口服液对于减少羔羊奶结的发生有明显作用，第2组药物四仙莪棱香附散治疗羔羊奶结效果最佳．     

Sugar beet rotation effects on soil organic matter and calculated humus balance in Central Germany

In order to quantify the influence of land use systems on the level of soil organic matter (SOM) to develop recommendations, long-term field studies are essential. Based on a crop rotation experiment which commenced in 1970, this paper investigated the impact of crop rotations involving increased proportions of sugar beet on SOM content. To this end, soil samples were taken in 2010 and 2012 from the following crop rotation sequences: sugar beet–sugar beet–winter wheat–winter wheat (SB–SB–WW–WW = 50%), sugar beet–sugar beet–sugar beet–winter wheat (SB–SB–SB–WW = 75%), sugar beet–grain maize (SB–GM = 50%) and sugar beet-monoculture (SB = 100%); these were analysed in terms of total organic carbon (TOC) and microbial biomass carbon (MBC) content, MBC/TOC ratio and the TOC stocks per hectare. In addition, humus balances were created (using the software REPRO, reference period 12 years) in order to calculate how well the soil was supplied with organic matter. In the field experiment, harvest by-products (WW and GM straw as well as SB leaves) were removed. After 41 years, no statistically significant differences were measured between the crop rotations for the parameters TOC, MBC, MBC/TOC ratio and the TOC stock per hectare. However, the calculated humus balance was significantly affected by the crop rotation. The calculated humus balance became increasingly negative in the order SB–SB–WW–WW, SB–SB–SB–WW, SB monoculture and SB–GM, and correlated with the soil parameters. The calculated humus balances for the reference period did not reflect the actual demand for organic matter by the crop rotations, but instead overestimated it.     

猪丹毒

猪丹毒又称“红热病” “打火印”或“钻石皮肤病”,是一种急性的、热性的传染病,引起该病的病原为红斑丹毒丝菌,可以通过伤口感染人,也是一种人兽共患病。患病猪的临床表现形式大致可分为3种类型,即急性、亚急性和慢性型。急性型病例主要的表现为出现明显的败血症症状。亚急性型病例则是在四肢、胸腹部、背部等皮肤处出现红色菱形、方形或圆形疹块。慢性型则多以出现关节炎和心内膜炎引起的关节肿胀、运动障碍和心跳加速、呼吸急促为主的临床表现;有时还可见皮肤坏死。红斑丹毒丝菌的最易感动物是猪,没有品种和年龄差异。使用疫苗是最佳的预防手段,治疗方面除使用抗生素外还可以选择中药方剂进行治疗。