稗草影响大豆根瘤固氮的机制研究之一——水分干扰机制

在全自控人工气候室内系统研究了稗草对大豆根瘤固氮的水分干扰机制。稗草在每盆3株的密度下使播后53-63淹水期间及淹水期后1天的大豆单株根瘤固氮速率平均分别比无草对照提高了16.6%和30.0%,在每盆10株的密度下分别使其比无草对照提高了34.2%和67.5%。在不淹水的情况下播后30-70天,稗草在每盆3株和10株的密度下使其所在处理的平均日蒸发蒸腾量分别比无草对照增加了23.8%和50%,削弱了水分对大豆植株的供应水平;而土壤水分供应水平从其田间持水量的80%降低到35%时,大豆根瘤的氧气传导速率从0.38cm/s下降到0.20cm/s,净光合速率从18.81mg/dm#+2/h降低到4.04mg/dm#+2/h,每盆10株稗草对大豆单株结瘤量及根瘤固氮酶活性的抑制率则分别从8.2%和17.8%升高到18.3%和19.6%,其对大豆单株根瘤固氮速率的抑制率也随之从21.5%上升到33.1%。证明通过干扰土壤水分供应水平,首先影响大豆植株的同化物产量及根瘤内的氧气供应,进而影响大豆植株的根瘤形成和根瘤固 氮酶的固氮活性,是稗草影响大豆根瘤固氮的主要机制之一。     

Economic implications of herbicide resistance and high labour costs for management of annual barnyardgrass (Echinochloa crus-galli) in Philippine rice farming systems

Implications of increasing labour costs and the development of herbicide resistance for profitable weed management in Philippine rice farming systems are investigated. The study employs RIMPhil (Resistance and Integrated Management in the Philippines), a bioeconomic simulation model developed to provide a comprehensive assessment of integrated weed management programmes for the control of annual barnyardgrass (Echinochloa crus-galli) in rice crops. Results indicate that herbicide application will become increasingly economically attractive, relative to manual weeding, as labour cost increases. This is important since urban migration in the Philippines continues to increase the scarcity of rural labour. Results also show that the onset of herbicide resistance results in substantial losses in farm profit. It is worthwhile for farmers to take management actions to prevent or delay the onset of herbicide resistance, provided that these changes are effective and not too costly. The study highlights the complexity of decision making about integrated weed management on rice farms in the Philippines.     

干湿交替灌溉条件下不同种稗草对水稻光合特性和产量的影响

以两优培九和南粳9108为试验材料,自水稻移栽至成熟分别与无芒稗(T1)、稗(T2)、西来稗(T3)和光头稗(T4)共生,以无稗草水稻处理(CK)为对照,研究干湿交替灌溉条件下不同稗草对水稻光合特性和产量的影响。结果表明,稗草对水稻产量的干扰因稗草种和水稻品种的不同而异。稗草种间干扰强度表现为T3T1T2T4,两优培九减产率小于南粳9108。T1、T2、T3和T4处理后两优培九的减产幅度分别为11.16%~13.78%、10.19%~10.60%、19.00%~23.79%和0.50%~1.57%,除T4外其他处理较对照显著降低;南粳9108的减产幅度分别为38.44%~45.51%、31.29%~36.86%、54.88%~60.65%和8.28%~15.14%,均达显著差异。T1、T2和T3处理后对两优培九叶面积指数和叶绿体色素含量无显著影响,但使南粳9108的叶面积指数降低和叶绿体色素含量增加。4种处理还显著降低了水稻冠层的透光率、剑叶光合速率、蒸腾速率和气孔导度以及干物质积累量。冠层透光率、光合速率、气孔导度、蒸腾速率和成熟期干物质积累量降低以及灌浆期叶绿体色素含量不同程度增加可能是水稻产量降低的重要原因。     

2种寒地稗草农艺学性状与细胞学研究

通过对黑龙江省内不同地方收集的2种寒地稗草(Echinochloa crusgalli(L.) Beauv.)的农艺学性状测试和细胞学研究,以期为寒地稗草资源的进一步研究和利用提供依据。2007年对2种稗草进行观察和试验,测定了生育期、株高、分蘖数、叶面积以及鲜、干重量等主要农艺性状,并对小区实际产量进行测试。结果表明:2种稗草农艺性状均为良好,在黑龙江省的气候条件下,粳稗(001)比大粒稗(026)表现更优;核型分析显示,2种稗草的核内染色体数目均为2n=54;大粒稗(026)2n=48m(3SAT)+6sm,粳稗(001)2n=36m+16sm+2st。     

Losses in Nitrogen Uptake by Tomato and Pepper Due to Infestation by the Weed Barnyardgrass

Abstract

Glasshouse experiments showed that the weed “barnyardgrass” (Echinochloa crus‐galli) competes for nitrogen (N) with tomato and pepper crops. Competition was more severe with pepper than with tomato, and greater in both crops the earlier the weed emerged or the longer it grew with the crops. This competition affected growth attributes, fruit yield and its components, and N uptake in both crops. Shoot N content was also affected in pepper. Significant damage to both crops occurred even when weed emergence was as late in the crop growth season as the beginning of flowering.     

Cyhalofop application timing and adjuvant selection for Echinochloa crus-galli control in rice

Field experiments were conducted in Stuttgart, Arkansas, in 2007 and 2008 to evaluate cyhalofop application timing and adjuvant selection for Echinochloa crus-galli L. Beauv. (barnyardgrass) control in drill-seeded rice. In the application timing experiment, cyhalofop was applied at 1 d prior to flooding (PREFL) and at 7, 14, and 21 d post-flood (POSTFL) as single (314 g a.i./ha) or sequential application (first application at 314 g a.i./ha followed by a second application 14 days later at 213 g a.i./ha). In the adjuvant study, the adjuvants evaluated were non-ionic surfactant (NIS) at 0.25% v/v, crop oil concentrate (COC) at 1% v/v, methylated seed oil (MSO) at 1% v/v, 28% urea ammonium nitrate (UAN) at 2.5% v/v, combination of NIS or COC or MSO with UAN, a proprietary blend of MSO/organosilicone (OSL)/UAN at 2.5% v/v, and MSO/OSL/UAN applied with NIS or COC. All adjuvants applied with cyhalofop (314 g a.i./ha at 14 d after flood establishment) resulted in E. crus-galli control and rice yields comparable to cyhalofop alone. In 2007, a single application of cyhalofop controlled E. crus-galli 98–100% by the end of the season, except in the 14-d POSTFL timing (90%). In 2008, E. crus-galli plants were more robust, and control was inconsistent and less than in 2007 (10–91%). At the PREFL timing, sequential applications were needed to improve end-of-season E. crus-galli control. The 7-d POSTFL timing was one of the best single application timings for increased E. crus-galli control and rice yield. Delaying the cyhalofop application to 21-d POSTFL resulted in unacceptable E. crus-galli control of 10 and 46% with single and sequential programs, respectively. Average rice yield was reduced almost three-fold when the first cyhalofop application was at 21-d POSTFL compared with 7-d POSTFL.     

稗对水稻生长和产量性状的影响及其经济阈值

稗Echinochloa crusgalli Beauv.在稻田的发生危害对水稻生产造成了严重威胁.为明确稗对水稻生长的影响及其经济危害允许水平,采用添加系列试验和模型拟合的方法研究了不同稗草密度下水稻各产量性状的变化规律.研究结果表明,水稻在稗的竞争干扰下,植株的分蘖数、有效穗数、千粒重及产量均随稗草密度的增加而逐渐降低.指数模型y=beax可以较好地拟合稗对水稻分蘖数(y=493.74e-0.0164x,P＜0.0001)、有效穗数(y=437.2e-0.0165x,P＜0.0001)及千粒重(y=21.876e-0.0006x,P＜0.005)的影响,而对数模型y=aLnx b拟合稗与水稻产量(y=-1250.4Lnx 6375.4,P＜0.0001)及产量损失(y=18.844Lnx 3.9182,P＜0.0001)间的关系最佳.稻田使用乙苄、丁草胺、禾大壮、二氯喹啉酸等药剂进行化学除草时,稗的经济危害允许水平在0.71%～1.10%之间,经济阈值在0.85株/m2左右.     

水稻化感作用潜力研究初报

6个化感水稻的化感作用潜力试验结果表明，不同品种水稻的化感潜力存在差异，6个供试水稻按其对稗草生长抑制率的大小依次为“Woo Co Chin Yu”＞“Kouketsumuchi”＞“AC1423”＞“CN-15”＞“Taichung Native 1”＞“YH-1”。提高化感物质的浓度，可相应增强对稗草生长的抑制作用。试验证实，水稻对稗草的化感作用具有密度依赖效应，表现为杂草密度加大，水稻化感抑制效果降低，这与资源竞争的结果恰好相反。     

Screening of allelochemicals on barnyardgrass (Echinochloa crus-galli) and identification of potentially allelopathic compounds from rice (Oryza sativa) variety hull extracts

Experiments were conducted to screen 23 known allelochemicals, including sinapic acid, and an equimolar mixture of the chemicals for potential allelopathy on barnyardgrass (Echinochloa crus-galli var. oryzicola), and to identify allelochemical(s) from hull extracts from three rice (Oryza sativa L.) cultivars. In a bioassay, the inhibitory effect was increased as the concentration of allelochemicals increased from 10⁻⁵ to 10⁻³ M. Ferulic, p-hydroxybenzoic, p-coumaric, and m-coumaric acids were the most active compounds and caused the greatest effect on seed germination, germination rate, and total seedling dry weight reduction. p-hydroxybenzoic acid (10⁻³ M; pH 4.1) showed the greatest inhibitory effect on the same parameters. HPLC analysis using three rice cultivars, Janganbyeo, Baekambyeo, and Labelle, showed that the concentration and composition of potentially allelopathic compounds depended upon the cultivar. Hull extracts from the allelopathic cultivar Janganbyeo contained higher levels of p-hydroxybenzoic acid than did those of the non-allelopathic cultivars Labelle and Baekambyeo. Nine compounds, including p-hydroxybenzoic acid (4.29 mg/g) in Janganbyeo, seven compounds including m-coumaric (0.43 mg/g) in Labelle, and five compounds including p-hydroxybenzoic acid (0.36 mg/g) in Baekambyeo, were detected. Preliminary identification by HPLC analysis resulted in peaks with retention times near those of standards, including p-hydroxybenzoic acid that was confirmed with EI/MS. It is suggested that these compounds may be, at least, a key factor in rice allelopathy on barnyardgrass, and the information presented may contribute to the development of naturally occurring herbicides.     

水稻、稗草叶片超微结构与光合性能关系的研究

应用电镜研究了水稻及稗草灌浆期旗叶片的超微结构，并测定了单位叶面积叶绿体含量，叶绿素含量及光合强度，结果表明，叶绿一及光龛中度不但和单个叶绿体光合膜系统有关，更主要的是受单位叶面积内总光合 影响。水稻叶肉细胞不且多为多环形复式细胞，胞质丰富，内含较多叶绿体，单位叶面积内光合膜系统发达，韧皮部各种细胞内存在发达的胞间连丝，有利于光合产物由叶肉细胞共质体运输至筛胞分子，水稻叶片光合性能较高。稗草叶肉细