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1.
Single nucleotide polymorphisms (SNPs) of cytokine genes have been found to be involved in the clinical outcome of Tuberculosis. The present study was aimed to identify the high risk genotypes in Tuberculosis patients and their household contacts. A total of 490 subjects were studied which includes 150 active pulmonary tuberculosis patients (APTB), 190 household contacts (HHC) and 150 healthy controls (HC). The SNPs of TNF-α (-308A/G), IL-10(-1082G/A) and IL-6(-174G/C) were performed by ARMs PCR. The IL-10 GA genotype showed significant association in APTB and HHC and was 2.3 times higher risk in APTB and 3.7 times in HHC compared to HCs. The A allele was found to be significantly associated with the risk of disease. The CC genotype of IL-6 was found to be significantly associated in APTB and an insignificant positive association in HHCs. The multifactor dimensionality reduction (MDR) analysis indicated that the genotypes of IL-6 were showing high risk with GA genotype of IL-10. In conclusion the gene interaction may be useful for identification of genotypes as biomarkers to distinguish high risk individuals. 相似文献
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豫谷6号是应用有性杂交技术,经连续南繁、温室加代选择育成的夏谷新品种。具有高产稳产、高抗倒伏、高度抗旱、综合抗病力强等优良特性,适宜中等以上肥力水、旱地种植。 相似文献
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Although the molluscicide Frescon is a strong neurotoxin to the Lymnaea stagnalis central nervous system in vitro, it is probable that the exposure of the whole animal to this molluscicide fails to result in central nervous system abnormalities: Frescon does not appear to reach the brain in sufficient quantity to disrupt its normal activity. However, only those Frescon analogs found to be neurotoxic were molluscicidal, suggesting some related mode, if not site, of action. Frescon and its analogs may act by affecting excitable tissues other than the nervous system (e.g., the snail musculature) by altering certain functional and/or structural membrane properties. 相似文献
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6-BA在葡萄植株体内的运转和分配 总被引:10,自引:0,他引:10
以葡萄为试材,研究了葡萄根、茎、叶、果等器官对6-苄基腺嘌呤(6-BA)的吸收及它在植株内的运转和分配。结果表明,对1年生葡萄幼苗茎部引入或叶片涂抹6-BA,1h后,在植株所有部位都有分布,其中以茎尖和根部浓度较高,表现为快速、非极性的运输特性。茎部引入5d之内,植株根内6-BA呈持续升高趋势,茎尖内6-BA虽然有波动,但是最后仍达到最大;而叶片涂抹则在涂抹后5h时,全株各部位6-BA浓度达到最大,5d后,除引入叶片外,其它部位几乎测不到6-BA。盛花后30d,果穗浸蘸和穗轴引入6-BA,2h后,果皮、果肉、种子中6-BA浓度达到高峰,以后逐渐下降,果穗浸蘸30d后,果实各部位均已测不到6-BA,穗轴引入30d后,果皮和果肉内仍能测到6-BA,但种子内已测不到。 相似文献
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结核分枝杆菌分泌蛋白ESAT-6、CFP10基因的克隆、鉴定及其原核表达 总被引:3,自引:0,他引:3
以人型结核分枝杆菌 H37RV株基因组 DNA为模板 ,应用 PCR法对 ESAT- 6和 CFP10基因进行扩增 ,产物经纯化后与载体 PMD18- T连接、转化及酶切鉴定 ,亚克隆到原核表达载体 PGEX- 6 P- 1,构建原核重组表达质粒 ,转化入大肠杆菌 BL2 1中 ,以 1mmol/L IPTG诱导 ,进行 SDS- PAGE电泳。结果表明 ,ESAT- 6和 CFP10基因表达的融合蛋白相对分子质量分别为 32 0 0 0和 36 0 0 0 ,与实测相符。重组结核杆菌分泌蛋白 ESAT- 6和 CFP10的成功表达为结核病诊断及重组疫苗的构建打下了基础 相似文献
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Background: CDK4/6 (Cyclin-dependent kinases 4/6) are the key promoters of cell cycle transition from G1 phase to S phase. Thus, selective inhibition of CDK4/6 is a promising cancer treatment. Methods: A total of 52,765 marine natural products were screened for CDK4/6. To screen out better natural compounds, pharmacophore models were first generated, then the absorption, distribution, metabolism, elimination, and toxicity (ADMET) were tested, followed by molecular docking. Finally, molecular dynamics simulation was carried out to verify the binding characteristics of the selected compounds. Results: Eighty-seven marine small molecules were screened based on the pharmacophore model. Then, compounds 41369 and 50843 were selected according to the ADMET and molecular docking score for further kinetic simulation evaluation. Finally, through molecular dynamics analysis, it was confirmed that compound 50843 maintained a stable conformation with the target protein, so it has the opportunity to become an inhibitor of CDK4/6. Conclusion: Through structure-based pharmacophore modeling, ADMET, the molecular docking method and molecular dynamics (MD) simulation, marine natural compound 50843 was proposed as a promising marine inhibitor of CDK4/6. 相似文献