贵州省某猪场体表脓肿病原菌的分离鉴定及药敏分析

为探明贵州省某猪场引起猪体表脓肿的原因,本研究对该猪场脓肿部位的脓汁进行细菌分离培养,并对分离所得的细菌进行革兰氏染色镜检、生化试验、药敏试验、16S rDNA序列分子分析及动物感染试验。结果显示,从脓汁中成功分离到了3株菌落形态不一的菌株,分别为金黄色葡萄球菌、化脓隐秘杆菌、停乳链球菌类马亚种,根据分离地点和时间将其分别命名为GZGP2018-1、GZGP2018-2和GZGP2018-3;GZGP2018-1菌株与NCBI上金黄色葡萄球菌的同源性高达99.9%,GZGP2018-2菌株与NCBI上化脓隐秘杆菌的同源性高达99.9%,GZGP2018-3菌株与NCBI上停乳链球菌类马亚种的同源性高达100%;3株分离菌株对头孢拉定、环丙沙星、磺胺间甲氧嘧啶和氟苯尼考较敏感,对青霉素类药物和红霉素耐药;3株分离菌株对试验小鼠均具有致死性。本研究为该猪场猪体表脓肿的发病原因、实验室诊断方法及日常防控提供了理论参考。     

集约化兔场高致病性痢疾志贺菌的分离与鉴定

石河子某大型规模化养兔场陆续发生一种高发病率、高死亡率的兔病。临床表现为皮下出现大小不一的脓肿,脓汁呈干酪样,尤其在颈部、背部和四肢较多发。食欲和精神无明显变化,病初体温升高,最后脓肿破溃,肌肉萎缩,消瘦而死。经细菌培养、形态学观察、动物实验、药敏实验以及生化特性鉴定和血清学实验最终确定为痢疾志贺菌。此菌对多种常用抗生素能产生耐药性,但对红霉素及部分人医用药品较敏感。     

Deep Gluteal Abscess in a Zebra (Equus burchelli boehmi)

A male zebra aged about 7 years, weighing approximately 250 kg showed signs of lameness on its hindquarter. It was treated by the zoo veterinarians symptomatically, and it recovered. However, there was recurrence of the symptoms after 1 month. The animal was treated with fluid, electrolyte, antibiotic, and analgesic therapy. The hematology and serum biochemistry profiles were tested and found within the normal range. The animal was tranquilized, and physical and external examinations were conducted. Radiological examination of hoof ruled out any chances of laminitis or any other hoof deformities. Per-rectal examination along with flexion and extension of the hind limbs could not reveal any abnormalities. However, there was no improvement, and finally it died. Postmortem examination revealed an internal deep gluteal abscess. This is a rare report of deep gluteal abscess in a zebra. The difficulties encountered in diagnosing the case and its serious possible complications and possible methods of diagnosing have been described, which may help future equine and wildlife vets in diagnosing such cases successfully and may help in saving the precious lives of the patients suffering from such conditions.     

利福平在脓肿病灶内的残效期及其耐受菌株的分离鉴定

将伪结核棒状杆菌人工接种５０只实验小鼠,４周后用利福平对其中存活的２７只进行治疗试验,疗程为１周。然后每周解剖３只实验小鼠,检测药物在其脓肿病灶内有效杀菌作用的持续时间即残效期;同时对耐受该药物的菌株进行分离鉴定。结果发现：停药后第８周病灶内不仅仍有药物残存,而且仍然具有强大的杀菌作用;同时由４只小鼠的脓肿病灶分离到接种菌,经鉴定确认均为利福平耐受菌。表明伪结核棒状杆菌对利福平极易产生耐受性。     

Brain microabscesses in a porcine model of Staphylococcus aureus sepsis

Background

Sepsis caused by Staphylococcus aureus often leads to brain microabscesses in humans. Animal models of haematogenous brain abscesses would be useful to study this condition in detail. Recently, we developed a model of S. aureus sepsis in pigs and here we report that brain microabscesses develop in pigs with such induced S. aureus sepsis.Twelve pigs were divided into three groups. Nine pigs received an intravenous inoculation of S. aureus once at time 0 h (group 1) or twice at time 0 h and 12 h (groups 2 and 3). In each group the fourth pig served as control. The pigs were euthanized at time 12 h (Group 1), 24 h (Group 2) and 48 h (Group 3) after the first inoculation. The brains were collected and examined histopathologically.

Results

All inoculated pigs developed sepsis and seven out of nine pigs developed brain microabscesses. The microabscesses contained S. aureus and were located in the prosencephalon and mesencephalon. Chorioditis and meningitis occurred from 12 h after inoculation.

Conclusions

Pigs with experimental S. aureus sepsis often develop brain microabscesses. The porcine brain pathology mirrors the findings in human sepsis patients. We therefore suggest the pig as a useful animal model of the development of brain microabscesses caused by S. aureus sepsis.     

Abnormal regurgitation in three cows caused by intrathoracic perioesophageal lesions

Background

Three Brown Swiss cows with abnormal regurgitation because of a perioesophageal disorder are described.

Case presentation

The cows were ill and had poor appetite, salivation and regurgitation of poorly-chewed feed. Collection of rumen juice was successful in one cow, and in another, the tube could be advanced to the level of the 7th intercostal space, and in the third, only saliva could be collected. In one cow, oesophagoscopy revealed a discoloured 10-cm mucosal area with fibrin deposits. Thoracic radiographs were normal. The cows were euthanased and examined postmortem. Cow 1 had a large perioesophageal abscess containing feed material at the level of the thoracic inlet, believed to be the result of a healed oesophageal injury. Cow 2 had an abscess between the oesophagus and trachea 25 cm caudal to the epiglottis with the same presumed aetiology as in cow 1. Cow 3 had a mediastinal carcinoma that enclosed and constricted the oesophagus.

Conclusions

Abnormal regurgitation in cattle is usually the result of an oesophageal disorder. Causes of oesophageal disorders vary widely and their identification can be difficult.