Activated coagulation times of normal cows

The Activated Coagulation Time (ACT) was determined on 10 normal cows. Duplicate samples were taken from each cow. The mean ACT was 145 seconds with a range of 120-180 seconds. Standard deviations were 18 and 13 for the first and second sampling, respectively. Repeatability of the ACT was acceptable.     

肾上腺皮质肿瘤良恶性鉴别方法探究

肾上腺皮质肿瘤（Adrenocortical Tumor,ACT）是一种常见的肿瘤,存在于人体的内分泌系统中,并且是引发肾上腺皮质癌（Adrcnocortical Carcinoma,ACC）进而导致死亡的主要原因.经典的＂Weiss系统＂在诊断明确性上存在种种无法解决的问题,但随着科技水平的发展,人们开始探索新的途径来鉴别ACT的良恶性,于是开始有了影像学和分子生物学技术等特异性的检查方法,从而进一步增强了诊断的明确性.现通过对比的方式,探究肾上腺皮质肿瘤（Adrenocortical Tumor,ACT）影像学检查和分子生物学技术检查的结果,从而能更准确地鉴别其良恶性.     

鸭梨肌动蛋白2基因克隆和表达分析

根据其他植物肌动蛋白2基因(Actin2)的保守序列设计一对简并性引物,从鸭梨叶片中克隆到1个编码肌动蛋白的基因,命名为PbACT2。PbACT2基因cDNA全长为1 180bp,开放阅读框1 134bp,编码377个氨基酸。氨基酸比对分析表明,该基因编码的氨基酸与其他陆生植物肌动蛋白基因具有较高的同源性。研究证明,PbACT2在鸭梨不同器官和不同发育时期的表达基本一致,说明该基因的表达较为稳定。     

Determination of Normal Values Using an Automated Coagulation Timer for Activated Coagulation Time and its Application in Dogs with Hemophilia

The present study was performed to determine normal values for the Medtronic HemoTec automated activated coagulation time (ACT) analyzer (Medtronic HemoTec Inc, Parker, CO, distributed in Switzerland by Convergenza AG, Vaduz, Liechtenstein), and to evaluate its ability to detect dogs with hemophilia. ACT was measured in 43 healthy dogs presented to the Companion Animal Hospital, University of Bern, Bern, Switzerland, with the Medtronic HemoTec ACT analyzer to determine normal values. The mean +/- 2 standard deviations (SDs) of the values obtained was defined as the normal range. ACT was measured 8-10 times on the same day in 6 dogs to determine repeatability. ACT also was measured in 11 dogs with hemophilia and compared with a conventional visual ACT measurement test and with the activated partial thromboplastin time (APTT). ACT values of the 43 dogs used to determine normal values ranged from 66.5 to 97.0 seconds (mean, 79.3 seconds; SD, 7.35 seconds; median, 78.5 seconds). A range of 64-95 seconds (mean +/- 2 SDs) was defined as the normal range for the tested device. Repeatability was poor (r = 0.256). ACT values measured with the automated device did not correlate with ACT values measured with a conventional visual test or with APTT Sensitivity of the test was 90.9%, specificity was 98.0%, and accuracy was 96.7%. Variability in the test results was large and may lead to incorrect results. The automated measurement device was not superior to the conventional visual method in evaluating dogs with hemophilia.     

激活素、抑制素及其受体与动物生殖作用的研究进展

激活素和抑制素均属于转化生长因子β(TGF-β)超家族成员的多功能生长和分化因子,具有多种生物学功能及广泛的生物学活性,其生理功能是通过受体与靶器官结合而实现的。作者主要就抑制素、激活素的结构特征、生理功能和对生殖的作用及其与受体的作用方式进行综述。     

白色金针菇原生质体单核菌系的建立及其遗传特性分析

利用原生质体技术获得的白色金针菇原生质体再生菌株,根据细胞核的分布与数目以及锁状联合的有无,选出25株单核菌株,对峙试验表明,这些单核株分为核基因型不同的两种类型。两种类型的单核株和始发双核菌株的菌落形态、生长速度、胞外漆酶活性和药物抗性等性状指标均有明显差异,表明生成原生质体的过程中,所再生的单核株发生了变化。     

湖南永州奈李果实一种新病害的病原鉴定

[目的]明确危害湖南永州奈李果实新发生病害的病原物种类,为更好地识别并防控该病害提供理论依据.[方法]用常规分离方法对湖南永州奈李果园发病样品进行病原菌分离,通过形态学特征观察,ITS、TUB、CAL和ACT序列和系统发育进化树分析及致病性测定,对分离获得的病原菌进行鉴定.[结果]分离获得的病原菌形态学特征与大豆拟茎点霉(Phomopsis longicolla)一致;其ITS、TUB、CAL、ACT序列与P.longicolla同源性分别为98%、99%、99%和98%.室内人工接种病原菌3~5 d后果实形成椭圆形至不规则形褐色病斑、后转腐烂等症状,与田间果实危害症状一致.[结论]初步确定危害湖南永州奈李果实的病原菌为P.longicolla YZ.     

不同繁殖力山羊血浆中INHB、ACTA和FSH变化规律的研究

实验以高繁殖力的大足黑山羊和繁殖力较低的萨能奶山羊为研究对象,比较2个山羊品种在一个发情周期内血浆中抑制素B(INHB)、活化素A(ACTA)和促卵泡激素(FSH)的分泌变化规律,旨在研究这3种激素与山羊繁殖力之间的关系。结果表明:大足黑山羊和萨能奶山羊血浆中FSH的分泌与ACTA呈正相关(P<0.01,P=0.361),与INHB呈负相关(P=0.022,P=0.005),大足黑山羊在整个发情周期内血浆中FSH、ACTA平均浓度略高于萨能奶山羊(P>0.05),而INHB平均浓度显著低于萨能奶山羊(P<0.05)。从发情征状明显至排卵前这段时间内,大足黑山羊血浆中FSH平均浓度显著高于萨能奶山羊(P<0.05)。结果提示,大足黑山羊卵泡期比萨能奶山羊长,发情周期内INHB与FSH的差异可能是导致大足黑山羊和萨能奶山羊繁殖力差异的根本原因之一。