探究畜牧养殖疫病多发原因及有效抑制

本文从介绍常见动物疫病类型入手,针对疫病多发原因进行分析,并围绕增强疫病防控意识、营造良好防疫环境、实行科学饲养管理、采取病源抑制措施四个层面,探讨了关于动物疫病的有效抑制措施,以期为畜牧养殖与疫病防控工作提供参考价值。     

酶法检测大豆蛋白酶抑制因子的实验探讨

大豆蛋白酶抑制因子是大豆的主要抗营养因子，约占大豆蛋白质的6％。生大豆抗营养作用的40％是由蛋白酶抑制因子引起的。大豆蛋白酶抑制因子能抑制畜禽肠道内胰腺分泌的蛋白水解酶，如胰蛋白酶、糜蛋白酶和弹性蛋白酶等的活性，引起生长停滞、胰腺增生和肥大等。这种作用在幼年畜禽上尤为明显，减少了其饲养价值，限制了大豆和豆粕作为主要蛋白饲料的使用价值。因此，实际生产中，获取不同品种的大豆及其加工副产物中胰蛋白酶抑制因子的活性范围，以确定大豆和豆粕在饲料中的使用量，急需简便、快速、准确的胰蛋白酶抑制因子活性测定方法。     

Inhibition of HSP70 Gene Expression by Modified Antisense and Its Effects on Embryonic Sensitivity to Heat Shock

Experiments were performed to evaluate the efficiency of inhibition of HSP70 gene expression by antisense oligonucleotides complementary to the mRNA of HSPTO and to test the effects of inhibition of HSP70 gene expression on subsequent embryonic sensitivity to heat shock. The results showed that transfection of pre-implantation embryos at 4-cell stage with 5 μM antisense oligo had no effect on in vitro blastocyst development. However, transfection with 10 to 40 μM antisense oligo had reduced in vitro blastocyst development to 15, 10% and 0; For the embryos which exposed to 40 μM As arrested at the 16-cell stage, there was no blastocyst formation within the heat shock groups. In contrast, transfection had no effect on embryonic sensitivity to heat shock, above 25% of embryos developed to blastocyst stage in control groups.     

冻干细胞检验鸡新城疫血清抗体的试验

冻干（醛化）红细胞和新鲜红细胞与同一份鸡血清分别做鸡新城疫（ND）的血凝（HA）和血凝抑制（HI）试验，二者血凝抑制价基本符合率达98．72％，相关系数为0．9288，冻干红细胞完全可以代替新鲜红细胞做血凝抑制试验检测鸡新城疫抗体效价。     

论电路中的干扰形成及干扰抑制技术

本文对电路中的噪声的形成进行分析,并对噪声干扰的抑制技术进行探讨。     

种植王草防制紫茎泽兰的技术研究

<正> 王草是象草和美洲狼尾草杂交育成的多年生直立型丛生性禾本科牧草,是我国热带和亚热带地区继象草之后又一种收割型速生性青饲料,原产于南美洲,与紫茎泽兰生活习性近似。王草作为饲料具有品质好、叶量多、适口性好、种植粗放、须根发达、分蘖快、侵占力强、适应性好等特点。王草生长快,凡是紫茎泽兰能生