除草剂生物测定的分析方法——平行剂量反应法

介绍一种除草剂生物测定的分析方法平行剂量反应法。该方法基于Logistic参数方程的原理 ,结合除草剂与植物间的作用特性 ,得出了描述测试植物与除草剂相互关系的参数 ,通过总结参数 ,来评价所测试的因素对药效的影响程度。如果所测定的因素单独应用不对测试植物产生明显影响时 ,可用此方法     

巨大芽胞杆菌E-1菌株对土壤氯嘧磺隆残留的降解效果

采用玉米对氯嘧磺隆敏感的原理,测定巨大芽胞杆菌E-1对土壤氯嘧磺隆残留的降解能力。结果表明降解菌用量为300 mL菌液/kg土时,对玉米根长的修复率为18.31%～62.48%;降解菌用量30 mL菌液/kg土时的修复率为11.78%～49.72%;降解菌用量3 mL菌液/kg土时,施用降解菌的处理与不施菌处理之间无显著差异。     

Effect of Different Bioassay Methods on Enzymatic Characteristics of Cotton Aphid,Aphis gossypfi Glover （Hemiptera：Aphididae）

In this study, the susceptibility of three populations of cotton aphid, Aph＆ gossypii Glover （Hemiptera：Aphididae） was assayed to imidacloprid （35SC） and thiametoxam （50WG）. The involvement of metabolic enzymes in the resistance strain of cotton aphid to the neonicotinoids was determined by the biochemical biomarkers and the resistance mechanism was determined as CaE. In another study, three different bioassay experiments were designed for detecting the susceptibility of cotton aphid to imidacloprid and thiametoxam and the effect of these two insecticides on the enzymatic activity of cotton aphid was assessed in the adult aphids treated with three different bioassay methods using a modified version of the FAO dip test, residue bioassay procedure and starvation method. Our findings suggested that the type of bioassay methods is very important when aphids＇ populations assess for the resistance against the neonicotinod insecticides. It has shown the starvation method is the most reliable method compared with other methods.     

分离自红棕象甲虫尸上的绿僵菌菌株鉴定及其对红脉穗螟的致病力

红脉穗螟是海南省椰子、槟榔等作物的重要害虫,已引起了严重为害,造成了一定的经济损失.自死亡的红棕象甲虫分离得到一株绿僵菌菌株Red-3,通过形态特征和分子生物学方法对其进行了鉴定,并测试了其对红脉穗螟的致病力.通过DNA提取、ITS序列扩增得到的片段长度为555 bp,经比对后发现该菌株与绿僵菌相似度达99％.实验室测...     

几个树种化感物质的初步分离与生物测定

对杉木、丝栗栲以及木荷叶片的化感物质进行初步分离，并用杉木种子进行生物测定结果表明，丝栗栲和木荷叶片经丙酮和乙酸乙酯萃取浓缩后，经乙醇提取的极性化感物质活性大于石油醚提以的弱极性化感物质活性，杉木叶片分别经丙酮和乙酸乙酯萃取浓缩后，经石油醚提取的弱极性化感物质活性大于乙醇提取的极性化感物质活性，这在一定程度上可以推断丝栗栲和木荷叶片化感物质对杉木种子发芽促进作用较强的是极性化感物质，杉木叶片化感物质对杉木种子抑制作用较强的是弱极性化感物质。     

金龟子绿僵菌对稻水象甲的致病性

以稻水象甲为靶虫,对３７株不同来源的绿僵菌菌株进行了致菌性检测,结果有６株致病率大于９０％对不同生育期的稻水象甲感病性测定表明,在寄主产卵期感病性最强,越冬期次之,新羽化代最 成虫产卵期施用绿僵菌,对控制稻不象甲有明显的作用。     

杀菌剂开发中的室内生物测定

针对杀菌剂开发中的室内生物测定,探讨了活体和离体的相互关系,生物测定类型的选择问题,同时从多角度阐述了杀菌剂生物测定的创新途径。     

绿僵菌生防制剂助剂的筛选

使用绿僵菌油剂防治草原蝗虫曾收到很好的防效。实验对绿僵菌油剂助剂中介质油、增活剂、紫外保护剂进行筛选。结果表明,用色拉油与煤油的混合油,作为绿僵菌孢子粉喷洒介质比较理想;增C,50mg/kg为最佳的增活剂及浓度;UV-14∶10为最佳的紫外保护剂和紫外保护剂与孢子粉的配比。用该油剂对蝗虫进行室内生物测定,求得LT50为5.23d。     

高效广谱Bt菌株的筛选

从感染Ｂｔ的小菜蛾和斜纹夜蛾虫尸中,分离得到３７个菌株,经过初筛、复筛,获得９８～１２、９８～２７、９８～２９三菌株,对斜纹夜蛾、小菜蛾均具有高毒力,并已通过生产中试。其中由９８～１２攻株生产的Ｂｔ增效制剂抑虫琳田间防效达８０％左右。     

In Vitro Activity of Lawsonia inermis （Henna） on Some Pathogenic Fungi

The present study was conducted to investigate antifungal activity ofLawsonia inermis （Henna plant）. Leaf samples of the plants were collected from Eastern Nile of Khartoum state, Sudan. Ethanol and petroleum ether extracts in various concentrations were obtained by maceration （cold method）. The extracts were bioassay in vitro to know their bioactivity to inhibit the growth of tested fungi. The cup plate agar diffusion method was adopted to assess the antifungal activity of the extracts against tested yeasts while agar incorporated method was used for other molds. Both extracts revealed anti fungal activity against all yeast strains except Pichiafabianii which was found resistant to both ethanol and ether extracts. The results displayed antifungal activity against tested fungi. Minimum mould concentration （MMC） of the extracts was determined. The obtained results revealed antifungal activity of henna leaves extracts which support the traditional use of the Henna in therapy of fungal infections. The possibility of therapeutic use of Sudanese henna as antifungal agents is recommended.