Resistance to Botrytis cinerea in Solanum lycopersicoides is Dominant in Hybrids with Tomato, and Involves induced Hyphal Death

Botrytis cinerea causes gray mold disease and affects hundreds of plant species, including tomato (Lycopersicon esculentum). The wild nightshade, Solanum lycopersicoides, is cross compatible with tomato and is more resistant to B. cinerea, thus representing a potential source for crop improvement. Tests involving droplet inoculation of detached leaves and spray inoculation of entire seedlings demonstrated that resistance to B. cinerea varies among S. lycopersicoides accessions, with S. lycopersicoides LA2951 being the most resistant accession tested. Expression of resistance in the intergeneric hybrid (L. esculentum cv. 'VF36' × S. lycopersicoides LA2951) suggested that resistance is at least partially dominant in tomato. A green fluorescent protein-tagged B. cinerea strain was used for confocal microscopic comparison of infection in leaves of S. lycopersicoides and tomato. Even though S. lycopersicoides supported spore germination, there was evidence for hyphal lysis and death 3 days after inoculation, at a time when lesions were expanding on susceptible tomato plants. The reduced frequency of B. cinerea lesion spread on S. lycopersicoides explains why this fungus produced fewer spores in this wild nightshade than in tomato.     

水稻半外卷叶突变体sol1的表型分析与基因定位

适度卷曲有利于提高水稻叶片的光合效率,增加植株光合产物的有效积累量。我们利用甲基磺酸乙酯(EMS)处理籼型水稻保持系西农1B,获得一个稳定遗传的水稻半外卷叶突变体。该突变体从十叶期开始各叶片逐渐向外卷曲直至半卷状,并伴随茎秆半矮化和叶片披垂,暂被命名为semi-outcurved leaf 1(sol1)。与野生型(WT)相比,sol1的叶片卷曲指数均达到30%以上(P<0.01);倒一、倒二、倒三、倒四节节间长度和穗长极显著缩短,倒一、倒二、倒三叶的叶夹角显著或极显著增加;有效穗数、千粒重、每穗实粒数、结实率显著或极显著下降,一次枝梗数则增加11.3%(P<0.05)。sol1的蒸腾速率、胞间CO2浓度、气孔导度显著高于野生型。石蜡切片显示,sol1倒一叶的泡状细胞体积变小,数量显著增多,表皮细胞体积略微增大。遗传分析表明,sol1的半外卷叶性状受1对隐性核基因调控,定位于6号染色体标记JY6-3和JY6-10之间165 kb的物理范围内,共含15个注释基因。qRT-PCR结果表明,与泡状细胞相关的内卷基因和外卷叶基因RL14、Roc5、REL1在突变体sol1中呈不同程度的上调,NRL、BRD1、OsHox32、ADL1、LC2则呈不同程度的下调。研究结果为SOL1基因的克隆和功能研究奠定了基础。     

Unraveling the morphological patterns of a subantarctic eelpout: a geometric morphometric approach

Phenotypic variation in organisms depends on the genotype and the environmental constraints of the habitat that they exploit. Therefore, for marine species inhabiting contrasting aquatic conditions, it is expected to find covariation between the shape and its spatial distribution. We studied the morphology of the head and cephalic sensory canals of the eelpout Austrolycus depressiceps (4.5–22.5 cm TL) across its latitudinal distribution in South Pacific (45°S–55°S). Geometric morphometric analyses show that the shape varied from individuals with larger snout and an extended suborbital canal to individuals with shorter snouts and frontally compressed suborbital canal. There was size variation across the sampled populations, but that size does not have a clear latitudinal gradient. Only 8% of the shape variation relates to this size variation (allometry), represented by a decrease in the relative size of the eye, and a depression of the posterior margin of the head. There were spatial differences in the shape of the head, but these differences were probably caused by allometric effects. Additionally, 2 of the canals of the cephalic lateralis pores and the head shape showed modularity in its development. This study shows that the morphology of marine fish with a shallow distribution varies across distances of hundreds of kilometers (i.e., phenotypic modulation).     

Research on a Novel Inverter of VSC

In order to improve waveform performance of light HVDC system, a lot of switches which operate in high frequency are used, which causes large switching losses and results high cost. A new Buck type voltage source converter(BVSC) is proposed. The new BVSC comprises a dual-buck converter and a three-phase inverter operating in grid frequency. Only two high frequency switches are used, which results good output waveform performance. In order to reduce the high frequency switching losses, a novel improved double-frequency Buck type voltage source converter(DBVSC) is also proposed. An additional dual-Buck converter is added to the BVSC, the added dual-Buck converter deals with the fundamental power that operates in low frequency, and BVSC only deals with harmonic power that operates in high frequency. The output waveform is enhanced and the switching losses are reduced in DBVSC, and the power rating is also increased. The DBVSC is very suitable for large power rating application. The one cycle control theory is adopted to control the proposed DBVSC with analog circuit, which is very simple. The proposed DBVSC and control method are verified by simulation.     

红外光电开关在杨树对靶喷雾机中的应用

环境保护和可持续发展是21世纪农业发展优先考虑的问题,对作物的病虫害进行化学防治是植物保护的主要手段。截止目前,我国已有常用农药品种180种以上,但有关植保的研究大多集中在农药本身,对施药机械的研究开发进行得很少,这是我国农药应用效率不高的一个重要因素。由此而造成的环境污染也越来越严重,对农作物对靶喷药是植保机械的重要研究方向。为此,设计了远距离红外光电开关应用于杨树对靶喷雾中,实验结果达到了设计要求,减少了施药成本,降低了对环境的污染程度。     

高效小功率离线式开关待机电源

论述了TinySwitch系列开关电源控制器TNY253/254/255的性能和工作原理,开关电源控制器主要用于10W以下的离线式开关电源。其中,TNY253特别适于用作消费类电子产品中的低成本、高效率待机电源。同时,介绍了用TNY253组成的7.5V/1.3W电视机电路;而TNY255特别适用于计算机待机电源。因此,用TNY255组成的10W计算机待机电源电路。TinySwitch开关电源控制器还可应用于做充电器电源。     

水肥一体化智能调控设备智能液位开关设计

在对设施蔬菜生产中使用的水肥一体化设备进行智能灌溉控制设计中,监测混肥罐和回液桶中液位高低是非常必要的。现有液位检测普遍采用机械开关式液位浮子,工程实践中发现当进出水口与浮子过近,或者水纹波动显著时,浮子上下剧烈抖动会导致水泵随浮子的抖动而经常启停,对于启动电流较大的泵容易造成损伤。本文在软硬件设计中采用了计数器数字滤波电路以及单片机定时器中断,设计了一种替代简单液位浮子的实用智能液位开关。为了检验该设计的性能,在同等条件下,进行了不同液面、不同压力和水流量的对比试验。试验结果表明,计数脉冲发生电路中充电电阻和放电电阻为4.7 kΩ时,滤波后的输出信号比现场实际的数控信号时间迟延了2.11 s,经数字电路滤波后的输出信号已经完全消除了宽度小于2 s的所有干扰信号,并且具有很高的稳定性。迟延时间与计数电路、计数脉冲发生电路有关,通过电路设计调整滤波脉宽可使输出信号有效地滤除干扰成分。同时,利用该方法也可以根据不同工况,如不同功率的水泵设定不同参数滤除干扰信号,提高智能液位开关适用性。     

构建VED-SegNet分割模型提取鱼类表型比例

为提高鱼类表型分割精度和准确度,实现鱼类表型智能监测,该研究基于深度学习算法构建了VED-SegNet模型用于鱼类表型分割和测量。该模型将cross stage partial network和GSConv结合作为编码器（VoV-GSCSP）,保持足够精度的同时降低网络结构复杂性。另一方面,该模型采用EMA(efficient multi-scale attention module with cross-spatial learning)建立强化结构,加强编码器和解码器之间的信息传递,提高模型精度,并实现了8个表型类别的输出。采用自建的鱼类表型分割数据集对VED-SegNet模型进行了测试,测量结果中鱼类各表型比例与实际测量值相接近,表型最大平均绝对和平均相对误差为0.39%、11.28%,能实现无接触式提取水产养殖中鱼类表型比例。对比其他常见语义分割模型,平均交并比mean intersection over union,mIoU和平均像素准确率mean pixel accuracy,m PA最高,分别到达了87.92%、92.83%。VED-SegNet模型在环境复杂、多鱼重叠的...     

数字果树及其技术体系研究进展

果树是重要的农林植物。在万物智联时代,利用数字化、智能化、网络化技术建立数字果树技术体系,实现对果树生命、生产和生态复杂系统的高效感知、认知和智慧管控,对于果业实现数字化转型具有重要意义。该研究在数字植物技术范畴下,系统论述了数字果树的概念和内涵,提出了数字果树技术体系框架。重点在果树表型信息获取、环境数据获取、三维模型计算、数字育种、果树大数据和虚拟现实技术等方面综述了数字果树研究进展。从产业应用角度,综述了数字果树技术在树形管理、生长监测、种植管理、农技培训、品牌营销等方面应用效果和挑战。最后,展望了数字果树发展趋势、研究热点和技术突破方向,以期为数字果树的进一步发展提供思路与借鉴。     

装载机中数字电液比例控制系统的仿真

介绍了用全新的数字电液比例先导控制方向阀——高速开关阀取代传统的电液比例阀,与多路阀构成数字电液比例换向阀,实现对装载机的数字化电液比例控制。然后用Matlab仿真软件中的Simulink对高速开关阀进行动态特性仿真,证明通过改变它的占空比可以控制多路阀的流量,从而达到控制工作部件的运动速度。