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1.
为建立一种能够快速、灵敏和特异地检测J亚群禽白血病病毒(ALV-J)的SYBR Green Ⅰ荧光定量PCR方法,本研究以ALV-J原型病毒株HPRS-103的pol基因3′端与gp85编码基因之间的保守区域(5 258 bp~5 802 bp)为检测目的片段,构建重组质粒并作为靶基因,通过对其浓度、引物浓度和退火温度的优化,建立了ALV-J SYBR Green Ⅰ荧光定量PCR方法.结果显示该方法的最低检测限度为2.36×102拷贝/μL,比普通PCR高100倍;与其他禽源病毒无交叉反应;批内和批间变异系数均小于5%.表明本研究建立的方法具有良好的特异性、稳定性和灵敏性.采用该方法对100只临床病鸡进行检测,ALV的检出率为44%.随机选择10只感染阳性鸡,检测病毒基因在心脏、肝脏、脾脏、肺脏和肾脏中的分布与表达水平,结果表明ALV-J在各主要脏器中均有分布,但以肾脏中的病毒表达量最高. 相似文献
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Effect of dietary phosphorus levels on growth and body composition of crucian carp,Carassius auratus under indoor and outdoor experiments 下载免费PDF全文
Indoor and outdoor experiments were carried out simultaneously to evaluate the effects of dietary phosphorus levels on growth performance and body composition of crucian carp. Monocalcium was used to formulate extruded feed containing available phosphorus (AP) of 1.5 (D1), 4.7 (D2), 6.3 (D3) and 9.0 (D4) g kg?1. Twelve 500‐L recirculating tanks were assigned to four triplicate groups with each tank stocked with 15 juveniles (39.88 ± 0.14 g) for indoor experiment. Twelve polyethylene enclosures were assigned to four groups with each one stocked with 120 juveniles (44.08 ± 1.36 g). After 8 weeks of feeding, survival ranged from 96% to 100%. Weight gain, special growth rate, protein efficiency ratio and feed conversion rate did not show significant differences among dietary treatments in both experiments. Fish cultured in the enclosures grew better, although D1 and D2 treatments did not reach significant level. Crude protein and phosphorus content of the fish were not significantly affected by dietary phosphorus level. Moisture, crude lipid, crude ash and vertebrae phosphorus were significantly affected by different phosphorus levels. Based on vertebrae phosphorus, crucian carp cultured in the tanks and in the enclosures showed phosphorus deficiency under 4.7 g kg?1 and 6.3 g kg?1 AP, respectively. 相似文献
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《Journal of Crop Improvement》2013,27(1-2):75-91
Abstract Maize grain yields have increased dramatically over the past fifty years, and concurrently end-uses have proliferated requiring special quality characteristics. Plant breeders have developed many specialty types of maize, all of which are influenced by the agronomic practices used to produce the crop. Grain yield increases have resulted in lower protein concentration except when the yield increase resulted from nitrogen fertilizer application. Irrigation improves the biological value of protein, while higher nitrogen application rates alter the amino acid balance thereby reducing the nutritional value. Kernel breakage susceptibility and kernel density increase with higher nitrogen fertilizer application rates, and are reduced by irrigation. Extractable starch and oil concentration are largely influenced by hybrid choice, but small production practice effects have been documented. Essential mineral nutrient levels are often influenced by soil or foliar fertilizer application. The production of aflatoxin can be reduced by irrigation or other strategies to avoid water stress during grain fill. Although genetics usually exerts the largest effect on maize grain quality, agronomic practices are also important. 相似文献
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HJ-1卫星延寿期的CCD影像质量评价与可用性分析 总被引:1,自引:0,他引:1
HJ-1卫星发射升空投入运行以来,已在灾害与环境的快速监测与预报领域发挥了重要作用。HJ-1卫星目前已经成功运行3a多,属于超期服役状态。为了评价延寿期影像质量,该文从影像的几何校正精度、灰度特征和纹理特征3个方面对延寿期HJ-1A-CCD影像进行分析,并与正常寿命运行期的HJ-1ACCD影像、同时期的Landsat7 ETM+影像进行比较,分析结果表明:延寿期HJ-1A-CCD影像质量虽然有一定程度的下降,但其与正常寿命期影像质量接近,仍能较好地描述丰富的地物类型及地物细节信息,可以像正常寿命期影像一样,有效地用于环境监测、灾害监测、国土资源管理、城市规划、农业应用等方面。 相似文献
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Effects of Irrigation Patterns and Nitrogen Fertilization on Rice Yield and Microbial Community Structure in Paddy Soil 总被引:6,自引:0,他引:6
Water and nitrogen (N) are considered the most important factors affecting rice production and play vital roles in regulating soil microbial biomass, activity, and community. The effects of irrigation patterns and N fertilizer levels on the soil microbial community structure and yield of paddy rice were investigated in a pot experiment. The experiment was designed with four N levels of 0 (N0), 126 (N1), 157.5 (N2), and 210 kg N ha-1 (N3) under two irrigation patterns of continuous water-logging irrigation (WLI) and water-controlled irrigation (WCI). Phospholipid fatty acid (PLFA) analysis was conducted to track the dynamics of soil microbial communities at tillering, grain-filling, and maturity stages. The results showed that the maximums of grain yield, above-ground biomass, and total N uptake were all obtained in the N2 treatment under WCI. Similar variations in total PLFAs, as well as bacterial and fungal PLFAs, were found, with an increase from the tillering to the grain-filling stage and a decrease at the maturity stage except for actinomycetic PLFAs, which decreased continuously from the tillering to the maturity stage. A shift in composition of the microbial community at different stages of the plant growth was indicated by principal component analysis (PCA), in which the samples at the vegetative stage (tillering stage) were separated from those at the reproductive stage (grain-filling and maturity stages). Soil microbial biomass, measured as total PLFAs, was significantly higher under WCI than that under WLI mainly at the grain-filling stage, whereas the fungal PLFAs detected under WCI were significantly higher than those under WLI at the tillering, grain-filling, and maturity stages. The application of N fertilizer also significantly increased soil microbial biomass and the main microbial groups both under WLI and WCI conditions. The proper combination of irrigation management and N fertilizer level in this study was the N2 (157.5 kg N ha-1) treatment under the water-controlled irrigation pattern. 相似文献
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通过水管部门组织科技人员对河套灌区调研为基础,以农业综合节水为目的,在灌区的中游永济灌域隆胜乡境内建设了节水农业示范区,面积为0.28万hm2.节水农业改造工程主要措施:渠道防渗衬砌、平地缩块.节水农业改造工程2002年竣工后,在运行中监测了3年,得出灌溉水量平均减少了12.3%;对示范区水盐动态进行了分析. 相似文献
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AM真菌与施氮量对白术光合色素的影响 总被引:3,自引:0,他引:3
以非灭菌土为生长基质,采用盆栽试验和室内分析相结合的方法,研究了不同施氮水平下接种AM真菌摩西球囊霉(Glomus mosseae)对白术光合色素的影响。结果表明,接种AM真菌提高了白术根系菌根侵染率和植株的光合色素含量,进而使光合作用增强;AM真菌的接种效应因施肥量不同而变化,施氮量为0.3~0.45 g/kg土时效果最好;适氮量和AM真菌结合有交互正效应,有利于白术生长。 相似文献
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选用健康乌苏里幼公貉63只,随机分为7组(每组9只)。1~4组用以探讨适宜蛋白质水平,其蛋白质水平分别为31.54%、36.39%,40.37%和43.53%;5-7组用以探讨适宜能量水平,其能量水平分别为19589.78,21860.94和22600.04k J/kg。试验分育成前期和冬毛生长期两个阶段进行,饲喂同一营养水平的日粮。结果表明,育成前期饲喂蛋白质水平为36.39%的日粮,可获得理想的体重、体长及经济效益。 冬毛生长期,日粮蛋白质水平在31.54%~43.53%范围内,对幼貉的体重和体长无显著影响。育成期以饲喂能量水平为21860.94~22600.04 k J/kg的日粮为宜。育成前期适宜的蛋能比为16.1~16.6,冬毛生长期为14.0~14.4。 相似文献