仔兔补饲方法的比较试验

仔兔从18～20日龄采取随笼补饲和离笼补饲两种方法。两批试验结果表明:随笼补饲的仔兔断奶窝重、窝日增重及成活率均明显高于离笼补饲的仔兔,重要的是随笼补饲极大简化了烦琐操作程序,减少了工作量,更适合规模化养兔场应用。     

苜蓿品比试验研究

以新疆大叶苜蓿为对照品种进行品种比较试验，通过对苜蓿的出苗速度、生育期、产草量、抗寒性、抗病性及根茎直径与根茎入土深度等项指标调查研究结果表明，FunduleaⅠ苜蓿出苗速度高于对熙2．8％，第2，3年干草产量分别比对照增加16．4％与12．4％，成熟期比对照提前9～16d，且抗霜霉病。就抗寒性而言，FunduleaⅠ苜蓿第2年越冬率略低于对照新疆大叶苜蓿，第3年与对照品种相同，2种苜蓿干叶率相比，FunduleaⅠ苜蓿低干时熙品种新疆大叶苜蓿。     

耕牛血吸虫病检测方法比较试验

目的为了解几种检测耕牛血吸虫病的敏感性进行本试验。方法将经粪便毛蚴孵化法检出血吸虫病阳性的水牛(6头)，同时进行干卵聚酯薄膜环卵沉淀试验、间接血凝试验及快速诊断试纸盒检验的测试。结果以其检测结果与粪便毛蚴孵化法相比，阳性符合率分别为100％、100％和83％，3种方法间无显著差异。结论快速诊断试纸盒检测法以其操作简便且准确、快速、特异性高的特点，在动物血防普查工作中具有广阔的应用前景。     

Comparative studies of strains of infectious bovine rhinotracheitis virus isolated from latently infected calves

Three strains (479 C, 778 TL, 982 LE) of infectious bovine rhinotracheitis (IBR) virus isolated from latently infected calves were compared with the prototype strain of IBR virus (LA strain) in studies which included restriction endonuclease analysis, experimental infection, and reciprocal cross protection tests in cattle. From the restriction endonuclease analysis it appeared that the 3 "latent" viruses were derived from the same isolate, and that it differed slightly from the LA strain. However, latency does not seem to have affected the pathogenicity or the immunogenicity of the virus. This is demonstrated by the identical clinical and virologic response of calves subjected to experimental infection with the various strains under study, and by the finding that when the LA strain and a "latent" strain (982 LE) were tested in cross protection tests in cattle, they proved to be mutually protective.     

2015年华南4种林业期刊引文对比分析

为探讨林业期刊引文特点,对华南4种林业期刊《桉树科技》、《热带林业》、《广东林业科技》和《广西林业科学》2015年度所刊发的论文引文进行引文量、引文率、篇均引文量、引文类型、引文语种、期刊自引率等6个方面进行对比分析,结果表明:2015年4种林业期刊引文量为442 ～1 839条,平均1094条;篇均引文量7.8～16.4条,平均13.6条;除《热带林业》引文率为94.7％外,《桉树科技》等3种期刊引文率均达100％;均以期刊类引文最多,图书类次之,学位论文、标准、专利等其他引文最少,其平均所占比例分别为82.3％、10.3％和7.5％.4种林业期刊的引文语种为中文与英文两种,其所占比例分别为88.7％与11.3％;平均期刊自引率偏低,为4.3％,与我国农业科学类期刊平均自引率9.7％的差距较大.     

西方国家碳税收入使用政策比较及其启示

碳税收入使用政策是西方国家碳税制度设计的一项重要内容，其主要基于税收中性原则，将税收收入或投资于环保节能项目，或以税式支出方式降低纳税人负担，确保碳税收入的循环，实现节能减排的目标。在我国碳税制度设计的构想中，应借鉴西方国家税制经验，结合本国现实情况，科学合理地设计碳税收入使用政策，确保碳税调节功能的充分发挥，将碳税发展成培育战略性新兴产业的重要政策工具之一。     

两个林龄15个桉树无性系生长比较

对福建长泰岩溪林场两个林龄的15个桉树无性系进行调查分析，结果表明：5 a、15 a 时，各无性系树高、胸径、单株材积、公顷蓄积差异极显著。5 a 时 W7、DH32-27、EC1、DH32-22、209X 等为高产无性系；15 a 时 EC1、DH32-27、209X、W7、岩97、DH32-22、LH3、LH1为高产无性系。W5、LPT、LH22、W6等无性系生长缓慢，可能不适应闽南山地环境。选择这些适应闽南山地环境的优良无性系与 DH32-29等进行混系造林或嵌合造林，有利于增加桉树速丰林的遗传基因多样性。     

Estimation of Crown Temperature of Winter Wheat and the Effect on Simulation of Frost Tolerance

Accurate estimation of winter wheat frost kill in cold‐temperate agricultural regions is limited by lack of data on soil temperature at wheat crown depth, which determines winter survival. We compared the ability of four models of differing complexity to predict observed soil temperature at 2 cm depth during two winter seasons (2013‐14 and 2014‐15) at Ultuna, Sweden, and at 1 cm depth at Ilseng and Ås, Norway. Predicted and observed soil temperature at 2 cm depth was then used in FROSTOL model simulations of the frost tolerance of winter wheat at Ultuna. Compared with the observed soil temperature at 2 cm depth, soil temperature was better predicted by detailed models than simpler models for both seasons at Ultuna. The LT50 (temperature at which 50 % of plants die) predictions from FROSTOL model simulations using input from the most detailed soil temperature model agreed better with LT50 FROSTOL outputs from observed soil temperature than what LT50 FROSTOL predictions using temperature from simpler models did. These results highlight the need for simpler temperature prediction tools to be further improved when used to evaluate winter wheat frost kill.     

基于非洲猪瘟病毒标准物质的荧光PCR检测试剂盒初步评价

为探究非洲猪瘟病毒(African swine fever virus,ASFV)标准物质作为试剂盒评价体系的可行性,比较了市场上5种主流品牌ASFV荧光PCR检测试剂盒的检测性能。使用ASFV P72基因核酸标准物质作为模板,根据5种试剂盒说明书分别进行相应的荧光定量PCR检测,结合扩增曲线、Ct值,分析不同试剂盒的敏感性、可重复性以及所需反应时间。结果显示:5种试剂盒的阴性、阳性对照均成立,最低检测限均为5.9×10^-1拷贝/μL;4个厂家的试剂盒线性关系R2>0.98,其中最优的R²=0.994,离散度最小;各试剂盒的实际反应耗时与理论反应耗时均有一定差异(0.20~0.96 h)。结果表明,各生产厂家使用P72基因作为靶基因研制的ASFV荧光PCR检测试剂盒都可以使用ASFV标准物质作为评价体系,来评判试剂盒的检测性能。本试验为各实验室不同样品检测的ASFV荧光PCR检测试剂盒选择提供了一种可用的评价方法。