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1.
Protective efficacy of an H5/H7 trivalent inactivated vaccine produced from Re-11, Re-12, and H7-Re2 strains against challenge with different H5 and H7 viruses in chickens
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ZENG Xian-ying CHEN Xiao-han MA Shu-jie WU Jiao-jiao BAO Hong-mei PAN Shu-xin LIU Yan-jing DENG Guo-hua SHI Jian-zhong CHEN Pu-cheng JIANG Yong-ping LI Yan-bing HU Jing-lei LU Tong MAO Sheng-gang GUO Xing-fu LIU Jing-li TIAN Guo-bin CHEN Hua-lan 《农业科学学报》2020,19(9):2294-2300
We developed an H5/H7 trivalent inactivated vaccine by using Re-11, Re-12, and H7-Re2 vaccine seed viruses, which were generated by reverse genetics and derived their HA genes from A/duck/Guizhou/S4184/2017(H5 N6)(DK/GZ/S4184/17)(a clade 2.3.4.4 d virus), A/chicken/Liaoning/SD007/2017(H5 N1)(CK/LN/SD007/17)(a clade 2.3.2.1 d virus), and A/chicken/Guangxi/SD098/2017(H7 N9)(CK/GX/SD098/17), respectively. The protective efficacy of this novel vaccine and that of the recently used H5/H7 bivalent inactivated vaccine against different H5 and H7 N9 viruses was evaluated in chickens. We found that the H5/H7 bivalent vaccine provided solid protection against the H7 N9 virus CK/GX/SD098/17, but only 50–60% protection against different H5 viruses. In contrast, the novel H5/H7 trivalent vaccine provided complete protection against the H5 and H7 viruses tested. Our study underscores the importance of timely updating of vaccines for avian influenza control. 相似文献
2.
Mark D. Rawling Nicola Pontefract Ana Rodiles Ilektra Anagnostara Eric Leclercq Marion Schiavone Mathieu Castex Daniel L. Merrifield 《Journal of the World Aquaculture Society》2019,50(6):1108-1122
Fish were fed a single‐strain yeast fraction (SsYF; 2 g/kg) or a multistrain yeast fraction (MsYF; 0.8 g/kg) for 10 weeks. The results demonstrated significant (p ≤ 0.03) elevations in weight gain, specific growth rate, protein efficiency ratio, and feed conversion ratio in fish fed the yeast fraction‐supplemented diets. In the distal intestine, a significant elevation in microvilli density was observed after 5 and 10 weeks of dietary supplementation with MsYF and SsYF, respectively, compared to control fed fish (p < 0.001). A significant elevation (p = 0.02) in the perimeter ratio was observed in fish fed diets supplemented with the yeast fractions. After 10 weeks of feeding on the experimental diets, Rt‐qPCR demonstrated a significant downregulation (p < 0.05) in the stress response genes, heat‐shock protein 70 (hsp70) and proliferating cell nuclear antigen (pcna), in fish fed diets supplemented with the yeast fractions. Significant (p < 0.05) elevations in interleukin 1‐beta (il1β) and interleukin‐10 (il10) gene expression were observed in fish fed diets supplemented with the MsYF compared to the other dietary groups. These findings suggest that feeding an MsYF specifically at a lower incorporation rate < 1 g/kg, compared to a commercial SsYF at 2 g/kg, is effective in improving the intestinal health status and growth performance of European seabass. 相似文献
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Gustavo Pereira Valani Aline Fachin Martíni Laura Fernanda Simes da Silva Renata Cristina Bovi Miguel Cooper 《Soil Use and Management》2021,37(1):22-36
Integrated crop–livestock–forest is a promising strategy to improve soil quality. It comprises four different integrated farming systems: crop–livestock, crop–forest, forest–livestock and crop–livestock–forest. This work systematically reviewed studies about integrated crop–livestock–forest systems and soil quality. A total of 92 papers were retrieved from the Web of Science—Clarivate Analytics platform, and the following information was analysed: publication year, institution, region of the studied site, type of integrated system, soil type, tillage system, maximum soil depth and the soil quality indicators assessed. Most studies were published in the second half of the 2010s. Brazil is a prominent focus of research about soil quality and integrated crop–livestock–forest systems, with significant contribution from its central and southern regions. The Embrapa was the main publishing institution, present in over one‐third of the studies. Crop–livestock was the most common integrated system, Ferralsols was the most common soil group, and most of the studied soils were clayey. No tillage was the main tillage system. Most studies focused on the topsoil, assessing physical and/or chemical soil quality indicators. More emphasis on biological indicators of soil quality is required, as well as assessments integrating biological, physical and chemical indicators of soil quality. Future works should compare different integrated systems, including assessments deeper in the soil profile, especially in systems with the forest component, and also in sandy and silty soils. Soil quality indicators that have been rarely used should be further tested. Novel indicators should be added to better understand the promotion of soil quality by integrated crop–livestock–forest systems. 相似文献
5.
为掌握典型药物在农作物中的污染特征及健康风险,保障农产品质量安全,利用超声萃取-固相萃取-高效液相色谱/串联质谱(LC-MS/MS)技术,对围场县全县马铃薯中5大类(磺胺类、四环素类、氟喹诺酮类、大环内酯类及其他类)25种药物污染水平及富集效果进行了调查,并对健康风险进行了评价。结果表明,有20种目标物被检出,不同目标物检出率差异显著,检出含量范围为0.13~3.67μg·kg-1,单种污染物最高检出含量为17.0μg·kg-1。马铃薯对目标污染物的富集系数范围在0.01~39.6之间,最大富集系数为61.8。畜禽粪便是马铃薯中药物残留的主要来源,尽管整体检出水平和健康风险相对较低,但长期摄入与协同作用引发的潜在健康风险不容忽视。因此,仍需严控污染源头,推进畜禽粪便安全资源化利用,确保农田环境质量及农产品安全品质。 相似文献
6.
免疫剂量和母源抗体对禽流感重组鸡痘病毒活载体疫苗免疫效力的影响 总被引:4,自引:0,他引:4
利用表达 H 5亚型禽流感病毒 (AIV)血凝素基因的重组鸡痘病毒 (r FPV- HA)以不同剂量免疫 1日龄 SPF鸡、有或无母源抗体 (FPV、AIV H5)的商品鸡 ,并于免疫后 2 1d利用同亚型 AIV通过肌肉注射进行致死性攻击 ,通过检测免疫后 HI抗体应答、比较攻毒后发病率和死亡率评价免疫剂量和母源抗体对 r FPV- HA免疫效力的影响。结果发现 ,免疫后 2 1d,15 %~ 2 0 %的 SPF鸡和无母源抗体商品鸡可检出 HI抗体 ,而含母源抗体商品鸡检测不到 HI抗体。利用H5亚型 AIV致死性攻击后 ,10 3~ 10 6 PFU的 r FPV- HA可保护 95 %~ 10 0 %的 SPF鸡和无母源抗体商品鸡抵御强毒攻击 ,使之免于发病和死亡 ;而不同剂量 r FPV- HA接种的含母源抗体商品鸡有 80 %~ 90 %发病和死亡。结果表明 ,在较宽的免疫剂量范围内 ,r FPV- HA对 SPF鸡和无母源抗体商品鸡可提供良好的保护 ,显示出一定的应用前景 ;母源抗体影响 r FPV- HA诱导的免疫应答 ,且提高免疫剂量亦不能克服其干扰作用 ,这提示在实际应用中需优化免疫程序 ,避免母源抗体干扰。 相似文献
7.
lcylp@sohu.com 《勤云标准版测试》2006,(3)
采用生态系统健康的理论和评价方法分析评价农田生态环境问题具有重要的理论和现实意义,应用生态健康指数定量评价农田健康水平是生态系统管理的重要内容。以武川县农田为研究对象,选择水分、生物量、幅宽等代表性指标,分析了不同作物覆盖下农田生态健康指数。结果表明:不同种植模式间农田健康指数差别明显。播种期较早的小麦田最为健康,没有采用地膜覆盖的马铃薯田健康指数最低,而采用地膜覆盖的马铃薯田健康指数比较高。推广小麦栽培和覆膜栽培是北方农牧交错带农田很好的经营管理模式,具有推广价值,是缓解该地区环境的有效手段之一。 相似文献
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氟虫腈(锐劲特)对水稻害虫防治效果及增产效应 总被引:6,自引:0,他引:6
为了阐明新一代杀虫剂氟虫腈(锐劲特)对二化螟、三化螟、稻纵卷叶螟的防治效果及应用前景,进行了水稻盆栽试验、田间防效试验,并对施用锐劲特后水稻叶绿素含量、功能叶叶面积和产量进行了测定。结果表明,锐劲特施药后13天接虫的三化螟幼虫死亡率仍高达94.44%。在田间防效试验中,每公顷施用锐劲特80%WG有效成分24g防治二化螟,防止枯心效果达97.62%,防止白穗效果达94.44%,害虫死亡率达96.15%;每公顷施用锐劲特5%SC有效成分22.5g防治晚稻三化螟,保苗效果可达95.91%,害虫死亡率达99.04%;每公顷施用锐劲特5%SC有效成分30g防治稻纵卷叶螟,保叶效果达97.88%。叶绿素含量比对照增加32.4%;每公顷施用锐劲特80%WG有效成分36g时,功能叶叶面积增加16%~27.5%,并且杂交稻博优湛19比常规稻早糯3321和早熟早籼815对锐劲特更敏感。每公顷施用锐劲特5%SC有效成分22.5~37.5g时,水稻株高增加4.0~6.1cm,每稻丛穗数增加2.6%~31.18%,结实率提高19.43%~33.97%,水稻产量增加21.58%~28.19%。 相似文献
10.