全文获取类型
收费全文 | 184篇 |
免费 | 15篇 |
国内免费 | 13篇 |
专业分类
林业 | 6篇 |
农学 | 7篇 |
9篇 | |
综合类 | 67篇 |
农作物 | 24篇 |
水产渔业 | 15篇 |
畜牧兽医 | 75篇 |
园艺 | 2篇 |
植物保护 | 7篇 |
出版年
2024年 | 1篇 |
2023年 | 8篇 |
2022年 | 13篇 |
2021年 | 8篇 |
2020年 | 10篇 |
2019年 | 6篇 |
2018年 | 6篇 |
2017年 | 10篇 |
2016年 | 12篇 |
2015年 | 9篇 |
2014年 | 9篇 |
2013年 | 8篇 |
2012年 | 19篇 |
2011年 | 10篇 |
2010年 | 12篇 |
2009年 | 3篇 |
2008年 | 7篇 |
2007年 | 9篇 |
2006年 | 12篇 |
2005年 | 8篇 |
2004年 | 8篇 |
2003年 | 8篇 |
2002年 | 2篇 |
2001年 | 4篇 |
2000年 | 5篇 |
1999年 | 1篇 |
1992年 | 1篇 |
1990年 | 1篇 |
1988年 | 2篇 |
排序方式: 共有212条查询结果,搜索用时 15 毫秒
1.
采用体外批次培养法,研究在日粮中添加不同水平SBOS对绵羊瘤胃发酵功能的影响。SBOS的添加水平分别为基础日粮的0,0.4%,0.8%,1.2%,1.6%和2.0%等6个水平,即1个对照组(0水平组)5个试验组,每个组3个重复。结果表明:体外培养24 h,日粮中添加SBOS可以增加瘤胃液NH3-N、丙酸及总VFA、MCP浓度,提高培养底物NDF降解率,降低瘤胃液pH值、乙/丙比值,对瘤胃产气量、瘤胃液乙酸、丁酸浓度影响不显著。应用综合评定指标(MFAEI)对产气量、pH、MCP浓度、总VFA浓度、NH3-N浓度和NDF含量进行综合评定,得出添加量为1.2%的SBOS更有利于调控瘤胃发酵。 相似文献
2.
The test was aimed at determining the effects of adding MOS to diets with different concentrate to forage ratios on ruminal fermentation of sheep in vitro.The 4×6 two-factor experimental design was chosen.There were 4 diets with different concentrate to forage ratios(20:80, 30:70, 40:60 and 50:50) and added to 6 dosages MOS(0, 0.4%, 0.8%, 1.2%, 1.6% and 2.0%), respectively.The method of gas production in vitro was used to measure the total gas and CH4 production, IVDMD, IVCPD and IVOMD after cultured for 3, 6, 9, 12 and 24 h.The results showed that the gas(except 12 and 24 h) and CH4 production(except 9 h) were not impacted by the two factors(P>0.05).The IVDMD, IVCPD and IVOMD were significantly affected by concentrate to forage ratios(P<0.05), while the IVCPD at 24 h was significantly affected by MOS(P<0.05).Meanwhile, all the indexes were not influenced by the interaction of two factors(P>0.05).The IVDMD and IVOMD increased with the rising of concentrate approximately, while the IVCPD decreased with the rising of MOS roughly.The biggest associative effect was gained in 30:70 concentrate to forage diet when adding 1.2% MOS. 相似文献
3.
Effects of the prebiotic mannan‐oligosaccharide on feed deprived zebrafish: Growth and reproduction
下载免费PDF全文
![点击此处可从《Aquaculture Research》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Mohammad Navid Forsatkar Mohammad Ali Nematollahi Gholamreza Rafiee Hamid Farahmand Christian Lawrence 《Aquaculture Research》2018,49(8):2822-2832
This study was designed to investigate whether the prebiotic mannan‐oligosaccharides (MOS) can reduce the deleterious impacts of feed deprivation on growth and reproduction in zebrafish. In the growth performance experiment, juvenile fish were distributed in the following four treatments: normal‐control (NC), starved‐control (SC), normal‐prebiotic (NP) and starved‐prebiotic (SP). After 8 weeks, NP and SC fish showed the highest and lowest growth patterns, respectively among treatments. Standard length, specific growth rate and feed conversion ratio did not differ significantly between NC and SP treatments. Autochthonous lactic acid bacteria (LAB) were significantly higher in NP and SP than the NC and SP treatments. Feed restriction resulted in significantly lower concentrations of thyroxine. In the reproductive performance experiment, additional juveniles were fed as in the growth experiment. Crossing in the final week of the experiment resulted in successful spawning in only NP fish, which showed mature sperm and oocytes in histological examinations. The number of spermatozoa was significantly lower in the fish that experienced feed restriction; however, the oocytes of SP females were at the same maturation level to that observed in NC females. Sex‐steroids changed after both starvation and MOS supplementation where NC and SP showed no differences in the levels of testosterone and female's 17β‐estradiol. These results indicate that MOS supplemented diet reduced some side effects of feed deprivation (final weight and length, SGR, FCR and levels of LAB and T3), and suggest that supplementation of the diet with MOS may ameliorate some of the negative effects of feed deprivation in zebrafish. 相似文献
4.
植物低聚糖提取和生物活性鉴定 总被引:1,自引:0,他引:1
应用酶解法从小麦细胞壁中提取出低聚糖。经过活性鉴定,具有诱导大豆和小麦抗毒素产生,特别是从小麦代谢、细胞、植株等水平上改善该作物体内防御功能等生物作用。水解酶的种类、纯度、酶活值与所降解低聚糖的活性程度密切相关;高纯度、高酶活单位的水解酶提取的低聚糖,具有较强的生物功能。对来源不同的低聚糖进行了活性差异的比较,同时还讨论了其它因素——诸如小麦细胞的生理状态,低聚糖保存条件等对低聚糖活性的影响。 相似文献
5.
电渗析技术在大豆低聚糖溶液脱盐上的应用 总被引:3,自引:0,他引:3
从甜浆中提取的大豆低聚糖粗提液中含有很多的盐类,影响糖液的纯度。该试验通过离子交换膜辅助的电渗析法对大豆低聚糖模拟溶液进行脱盐处理,确定了较好的试验操作参数。试验结果表明:电渗析法对大豆低聚糖模拟液进行脱盐是可行的,且脱盐效果较好,脱盐率达到96.07%;该试验条件下较佳的工作电压为20 V,流量为60 L/h,样品稀释倍数为15倍。通过以上条件处理后,大豆低聚糖的保留率达到83.82%。 相似文献
6.
采用响应面法优化超声波辅助提取薏苡仁低聚糖的工艺条件。在单因素试验基础上,选取液料比、超声波时间以及超声波功率3个因素结合Box-Behnken试验建立数学模型,分析考察3个因素对薏苡仁低聚糖响应值的影响程度,优化工艺参数。各因素对薏苡仁低聚糖提取率影响程度从大到小顺序依次为:超声波功率超声波时间液料比。响应面设计法优化出其最佳超声波提取条件为:超声波温度70℃,液料比33∶1(m L/g),超声波时间27 min,超声波功率450 W。在该条件下,薏苡仁低聚糖提取率为0.94%,与模型预测值0.98%接近。说明使用响应面法优化超声波辅助提取薏苡仁低聚糖的工艺条件是可行的。 相似文献
7.
Zhao Xue Xiao-Meng Sun Cui Chen Xi-Ying Zhang Xiu-Lan Chen Yu-Zhong Zhang Shou-Jin Fan Fei Xu 《Marine drugs》2022,20(3)
Alginate oligosaccharides (AOS) have many biological activities and significant applications in prebiotics, nutritional supplements, and plant growth development. Alginate lyases have unique advantages in the preparation of AOS. However, only a limited number of alginate lyases have been so far reported to have potentials in the preparation of AOS with specific degrees of polymerization. Here, an alginate-degrading strain Pseudoalteromonas arctica M9 was isolated from Sargassum, and five alginate lyases were predicted in its genome. These putative alginate lyases were expressed and their degradation products towards sodium alginate were analyzed. Among them, AlyM2 mainly generated trisaccharides, which accounted for 79.9% in the products. AlyM2 is a PL6 lyase with low sequence identity (≤28.3%) to the characterized alginate lyases and may adopt a distinct catalytic mechanism from the other PL6 alginate lyases based on sequence alignment. AlyM2 is a bifunctional endotype lyase, exhibiting the highest activity at 30 °C, pH 8.0, and 0.5 M NaCl. AlyM2 predominantly produces trisaccharides from homopolymeric M block (PM), homopolymeric G block (PG), or sodium alginate, with a trisaccharide production of 588.4 mg/g from sodium alginate, indicating its promising potential in preparing trisaccharides from these polysaccharides. 相似文献
8.
9.
Algal-oligosaccharide-lysates prepared by two bacterial agarases stepwise hydrolyzed and their anti-oxidative properties 总被引:1,自引:0,他引:1
Twenty algal-oligosaccharide-lysates (AOLs), derived from six agars and four algal polysaccharide extracts (APEs), were treated
first with agarases with 250 or 500 agarase activity units (AU), which were produced from the agar-liquefying bacterial strain
Pseudomonas vesicularis MA103, named MA103-agarases. The AOLs were then treated with agarases (250 or 500 AU) derived from the agar-softening bacterial
strain Aeromonas salmonicida MAEF 108, named MAEF 108-agarases. Anti-oxidative properties of the AOLs were evaluated by five in vitro methods. The AOL obtained from the APE of Porphyra dentate, digested by 250 AU of MA 103-agarases, and by 250 AU of MAEF 108-agarases, designated as A250-Por, showed better results than the 19 other AOLs. This result is in accordance with the level of soluble total polyphenols
(STP) of A250-Por, which was also higher than the remainder of the AOLs tested. The AOL derived from the APE of P. dentate, digested by 500 AU of MA103-agarases and then 500 AU of MAEF108-agarases, and designated as B500-Por, displayed the second highest data in four potential evaluation methods, except in H2O2 scavenging capacity. In this study, certain agars or APEs digested by specific agarases can present an increasing antioxidative
capacity. These agars include Bitek agar, Agar powder, Bacteriological, Agar Bacteriological, and Guanghui agar, plus APEs
of Gracilaria sp. and Monostroma nitidum decomposed stepwise by two agarases. The fraction of polyphenols (<1 kDa) that were derived from A250-Por showed anti-oxidative activities on α, α-diphenyl-β-picrylhydrazyl (DPPH) assay and reducing power determination, while
the remaining four agar-lytic fractions obtained from A250-Por did not exhibit anti-oxidative activity. This phenomenon may suggest that anti-oxidative properties of AOLs originate
in polyphenols. Algal-oligosaccharide-lysates may have potential use as a health food. 相似文献
10.
大豆低聚糖分离纯化研究 总被引:1,自引:0,他引:1
大豆低聚糖能促进人体肠道内固有的有益细菌——双歧杆菌的增殖,从而抑制肠内腐败菌的生长,并减少有毒发酵产物的形成。是一类功能性低聚糖。综合论述了国内外工业上分离纯化大豆低聚糖的典型工艺过程,并对各单元操作中所用方法进行了比较和分析。 相似文献