Comparison of flagellin and an oil-emulsion adjuvant in inactivated Newcastle disease vaccine in stimulation of immunogenic parameters

The present study was designed to investigate the potential application of native (N) and recombinant (truncated modified [tmFliC] and full-length [flFliC]) flagellin proteins along with inactivated Newcastle disease virus (NDV). Fifty six SPF chickens were immunized twice with PBS (control), inactivated NDV (Ag), inactivated NDV/flFliC (AgF), inactivated NDV/tmFliC (AgT), inactivated NDV/N (AgN), commercial vaccine containing Montanide (Vac) and Vac/N (VacN), with a two-week interval. Blood was collected weekly and spleens were harvested after chickens were sacrificed. Interleukin-6 (IL-6) and tumor necrotic factor-α (TNF-α) gene expression in peripheral blood mononuclear cells were analyzed by Real-Time PCR. Antibody response was assessed by haemagglutination inhibition (HI). Cellular activity was quantified by MTT assay. Results showed that the most IL-6 and TNF-α gene expression was observed in AgF group (P < 0.01). The lowest gene expression among vaccinated groups was observed in Ag group for IL-6 and Ag and Vac group for TNF-α. The highest HI titer was observed in Vac, VacN, AgF and AgT groups. The AgF group showed the highest cellular activity (P < 0.01). In conclusion, flagellin-adjuvanted groups showed a pro-inflammatory effect and acted similarly to or better than the Vac group. Hence, flagellin can be proposed as a potential adjuvant for ND vaccine.     

闽南山地卷荚相思家系造林试验

为了筛选出适合闽南北部山地栽培的优良卷荚相思家系,华安金山国有林场优选9个卷荚相思家系进行造林试验,对不同家系林分的生长性状、适应性和抗逆性进行对比分析。结果表明,参试家系均表现出较强的抗风性和抗寒性,可以适应闽南北部区域环境。但不同家系的树高、胸径、材积和枝下高差异显著或极显著,其中Z5和Z7家系的生长表现优于其他家系,其树高、胸径、材积、枝下高比群体均值分别提高10.80%～11.59%、11.85%～15.05%、35.62%～41.78%和10.98%～13.41%。Z5和Z7家系初步评价为优良家系,可作为卷荚相思在闽南北部推广造林的推荐材料。     

施用锌肥和遮阴互作对花生生长发育、抗病性及产量的影响

采用大田裂区试验，研究了施用锌肥和不同遮阴程度互作对花生生长发育、抗病性及产量的影响。结果表明，与不施锌肥相比，施用锌肥能提高花生不同部位锌含量、增加叶片SPAD值，提高花生叶片中可溶性糖、蛋白质和生长素含量，减少花生病害的发生，平均降低7.1个百分点，花生产量平均增加19.4%。相同施锌水平下，随着遮阴程度的增加，花生不同部位锌的含量和不同生育期叶片SPAD值以及花生叶片中可溶性糖、蛋白质和生长素含量呈增加趋势，花生的发病率比不遮阴对照增加4.8、10.2个百分点，花生产量平均降低16.5%、10.0%。在30%、70%的遮阴条件下，施用锌肥的花生产量比不施锌的分别提高21.1%、25.0%。本试验条件下，施用七水硫酸锌30 kg/hm2，使花生具有较强的抗低温寡照能力及抗病性能，增产显著，可在花生产区推广应用。     

多酚类化合物协同抗氧化性研究进展

多酚类化合物是一种大量存在于植物体内的物质，因其具有优良的抗氧化、抗癌、抗衰老等 性能而受到研究者们的广泛关注。多项研究表明具有抗氧化性能的物质之间大多具有协同作用，两 种或两种以上的物质化合，其抗氧化能力往往强于单一物质的抗氧化能力。近年来，许多研究者将目 光聚焦到多酚类物质与小分子物质化合所具有的协同抗氧化能力的研究上并做出多项试验。本文论 述了多酚类化合物的抗氧化机理及协同抗氧化机理，并以茶多酚与维生素C、维生素E 化合为例对协 同抗氧化进行论述。     

Use of netting and whitewash spray to protect papaya plants against Nivun Haamir (NH)-dieback disease

A disease causing the decline of papaya (Carica papaya) plants was noticed in the Jordan Valley in 1982. The disease caused severe yellowing of the upper leaves and dieback of the apex. The disease was not transmissible mechanically and continued efforts to associate viruses and viroids have so far been unsuccessful. Epidemiological observations suggested that the disease is airborne and probably caused by a mollicute. In order to test this hypothesis, plots were covered by an insect-proof net and with white nets providing 15, 30 and 50% shade. The 30%, 50% and insect-proof nettings provided complete protection, and the 15% netting reduced disease incidence to <2%, compared with>37% in the uncovered control plot. Spraying plants at weekly intervals with a commercial whitewash solution was also found to be effective in reducing disease incidence. The advantages of using a range of netting field treatments as a simple means for obtaining information on the epidemiological nature of a new disease are described.     

奶牛繁殖疾病的调控

本文综述了奶牛繁殖疾病的发病原因和调控措施，分析认为奶牛的营养状况是影响奶牛繁殖力的重要因素。理想的奶牛管理应做到整个泌乳期始终监测奶牛的体况和奶牛日粮中微量元素的维生素的含量，避免营养素的不平衡而引发的奶牛繁殖障碍，同时应避免奶牛过肥或过瘦。适当补充某些维生素和矿物质十分重要。     

真核表达NDV F基因重组质粒的构建及其在CEF细胞中的转染

通过PCR方法自含NDVZF基因的克隆质粒中扩增NDVF基因 ,将其与真核表达载体pcDNA3..1/V5_His_TOPO重组。经核酸内切酶酶切 ,阳性克隆鉴定准确无误 ,核酸序列测定结果与原始基因比较 ,同源性大于 99% ,启始密码和终止密码未出现变异 ,将该重组质粒命名为pcDNANDVZF。将pcDNANDVZF在脂质体作用下转染CEF细胞 ,用间接免疫荧光试验检测 ,结果证明pcDNANDVZF可在CEF细胞中大量表达F蛋白。     

频域心电图对冠心病诊断价值的初步探讨

对５０例冠心病和５０例随机做平板运动试验（ＴＥＴ）者作频域心电图（ＦＣＧ）对比分析探讨ＦＣＧ对冠心病的诊断价值。结果表明：单纯根据ＦＣＧ异常诊断冠心病有较高假阳性（６５．２２％）。如将ＦＣＧ的阳性指标作综合判断，虽然敏感性降低（８８．００％降至７０．００％）。假阳性都明显降低（６５．２２％降至２６．００％）。认为ＴＥＴ的特异性较ＦＣＧ高。若两者均为阴性，则冠心病可能性很小。