破壁蜂花粉对蛋鸡免疫功能及肠道菌群的影响

试验将槐花蜂花粉进行振动磨超微粉碎破壁,分别按1 g/kg、5 g/kg、10 g/kg三个不同剂量混到蛋鸡饲料中,从7日龄开始饲喂至50日龄,每个剂量分两个试验,试验一用新城疫Ⅳ系疫苗免疫雏鸡,利用β-微量法监测血清中的血凝抑制抗体效价的动态变化;试验二用大肠杆菌油乳苗免疫雏鸡,利用间接血凝法监测血清中大肠杆菌抗体效价的动态变化。分别定期称重捕杀取脾、胸腺、法氏囊,计算免疫器官指数,取肠道内容物计数大肠杆菌和乳酸菌,测定肠道内pH值。结果表明,不同剂量的破壁蜂花粉均能不同程度地提高病毒抗原性抗体效价和菌体抗原性抗体效价,对雏鸡免疫器官的发育以及肠道内环境、菌群的平衡也有一定影响。     

Effect of YIGU capsule on IGF-I mRNA and protein expression in rat osteoblasts in vitro

AIM: The effects of YIGU capsule on proliferation and IGF-I mRNA protein expressions in osteoblasts were studied. METHODS: (1) Forty 12-month old Sprague-Dawley female rats were divided randomly into four groups (YIGU capsule high dose group, medium dose group and low dose group; saline group), the drug-containing serum and control serum were prepared. (2) The new-born Sprague-Dawley rat osteoblasts were cultured with different YIGU capsule drug-containing serum at different concentrations and different exposure time. MTT method was used to observe proliferation of osteoblasts. (3) RT-PCR method was used to measure the relative IGF-I mRNA levels and ELISA method was used to measure IGF-I secretion at different exposure time. (4) ELISA method was used to measure IGF-I secretion at different exposure time. RESULTS: (1) Proliferation of osteoblasts was more than the control groups after 48, 72 and 96 h, respectively (P<0.01); (2) The relative IGF-I mRNA levels and IGF-I protein expression were higher than those in control group after 48, 72 and 96 h, respectively (P<0.01 or P<0.05). CONCLUSIONS: It was suggested that YIGU capsule drug-containing serum promoted proliferation, IGF-I mRNA and protein expression. These results may be parts of the mechanisms of YIGU capsule to prevent and treat osteoporosis.     

次生幼龄林封山育林后乔木层物种结构变化研究

1999年在浙江省仙居县俞坑村的封山育林区设置20 m×20 m的固定样地2块,对连续5年的调查数据进行统计分析,以其对次生幼林乔木物种结构变化进行研究.分析结果,乔木层物种丰富度指数增加了7～11,乔木物种增加了2～5科,3～8属.自然恢复为以壳斗科、杉科、山茶科、樟科、胡桃科、安息香科等物种为建群种或共建种的次生常绿阔叶林、针阔混交林.     

氧化乐果和阿维菌素对大叶黄杨叶表蜡质层的影响

选用阿维菌素和氧化乐果喷施到大叶黄杨上,利用氯仿法测定了大叶黄杨叶表的蜡质含量。其结果表明,供试农药对大叶黄杨叶表蜡质层含量均有显著影响,其中以阿维菌素影响较大,氧化乐果影响较小。     

中国粮食仓储行业淘汰甲基溴行动

为切实履行我国政府对国际社会<关于消耗臭氧层物质的蒙特利尔议定书>的<哥本哈根修正案>所作出的承诺,中国粮食仓储行业在保障我国粮食安全储备的前提下,在"淘汰甲基溴,保护臭氧层"方面采取了一系列行动(包括调研、确定替代技术、培训、制定规程、建设示范库、研究替代技术等),以达到替代甲基溴的目的.淘汰甲基溴的实际行动,研究新的淘汰甲基溴替代技术,对增强人们保护臭氧层的意识,推广和应用替代技术和确保人类共同家园的可持续发展都具有重要的意义.     

红壤干旱过程中剖面水分特征与土层干旱指标

农田水分管理以及产量评估都需要对土壤作物受旱状况定量化和指标化。干旱强度和干旱程度结合才能完整地描述土壤作物干旱状况,土壤干旱强度I是土壤剖面失水速率的函数,干旱影响逐渐累积并增强就构成干旱程度D,据此提出了包含I和D二个指数的土层干旱指标表达模式。通过红壤小区种植玉米并在抽穗期开始设置连续干旱12～36 d等6个不同的处理,研究了红壤干旱过程中剖面水分特征和干旱指标。结果表明：供试红壤干旱过程中剖面40 cm以下含水率下降幅度很小,玉米主要利用了0～40 cm土层的水分,监测30～40 cm土层含水率的变化情况可以指示玉米受到干旱胁迫的程度。连续干旱25 d后40 cm以下土层含水率明显降低,玉米产量也显著下降,此时0～60 cm土层和30～40 cm土层的干旱程度D均为0.55,可用此指标作为灌溉的依据。     

Campylobacter spp. prevalence and fluoroquinolone resistance in chicken layer farms

Chicken is a major source of human campylobacteriosis. Chicken meat originates not only from broilers but also from spent layers; however, few reports have documented the prevalence and antimicrobial resistance of Campylobacter spp. in layers in Japan. Therefore, we investigated the prevalence and antimicrobial susceptibility of Campylobacter spp. in 47 layer farms in Japan. Fecal samples were collected from the youngest and oldest flocks on the farm, and Campylobacter spp. was isolated from 46/47 (97.9%) farms. Among the C. jejuni isolates, the resistance rates to ampicillin, tetracycline, and ciprofloxacin were 29.6%, 22.2%, and 19.8%, respectively. The ciprofloxacin resistance rate (7.3%) in C. jejuni isolated from old flocks was significantly (P<0.01) lower than that in young flocks (32.5%).     

半干旱黄土丘陵区小流域横断面土壤水分生态特征

在半干旱地区甘肃榆中北部中连川流域取一横断面,根据地形部位与利用条件布设11个测点,用土钻法监测土壤水分剖面分布与动态,测深到5m。各立地条件年内4月到12月土壤水分动态趋势多样。流域横断面的土壤湿度均较低,2～5m平均值一般都小于10％,其中以坡地苜蓿与半阳坡柠条林地最干燥。该地区土壤干燥化层是普遍存在的,不仅存在于人工植被,也存在于天然植被。径流富集叠加可显著改善土壤水分状况,隔坡水平梯田是其重要途径。退耕还草中要考虑各类草地的比例、苜蓿种植的适宜规模及其持续性问题。     

熊胆汁的定性检查

本文比较了熊(黑熊和棕熊)、牛、羊、猪、犬、兔、鸭等动物胆汁的外观性状,并对这些动物的胆汁及熊胆制剂中提取的胆汁酸成分进行了薄层层析比较。结果表明,熊胆汁层析图谱具有明显的特征性。将它们水解后层析,只有熊胆汁含游离的熊去氧胆酸。根据胆汁样品及其水解产物样品的层析图谱,即可鉴定商品熊胆及其制剂的真伪。     

饲喂高亚麻籽日粮的蛋鸡生产性能恢复研究

选择167只经4个月高亚麻籽饲喂后的产蛋鸡,以玉米豆饼为基础的日粮饲喂80d,观察其生产性能恢复情况。结果表明：高亚麻籽组的蛋鸡体重始终低于正常,产蛋率和体重几乎不能恢复。