Collembola in ecotoxicology—Any news or just boring routine?

Despite the uncontested significance of soils for human nutrition and drinking water quality, the majority of ecotoxicological testing is confined to aquatic test systems. Among the standardised tests for soils, the reproduction test with the springtail Folsomia candida is among the most widely used ones. First steps towards its standardisation were undertaken in the late 1980s. Here we review major advances that have been made since then, with respect to mechanistic, pragmatic and ecological aspects. Specifically we address the ecological relevance of any modifications of the standardised tests. We introduce a miniaturised version of the reproduction test which allows reducing the amount of soil per test unit to one third and the number of synchronised individuals to 40% as compared to the standard test. In addition, we developed an assay using Collembola eggs instead of synchronised adults. First results of a three-species test indicate that the presence of other species may affect choice behaviour. We point out a potential biased view of existing ecotoxicological data with Collembola due to the fact that most results refer to metal contamination. Finally, recommendations for future research are given, with special reference to avoidance and microcosm tests involving Collembola.     

CPUE standardisation and the construction of indices of stock abundance in a spatially varying fishery using general linear models

Construction of annual indices of stock abundance based on catch and effort data remains central to many fisheries’ assessments. While the use of more advanced statistical methods has helped catch rates to be standardised against many explanatory variables, the changing spatial characteristics of most fisheries data sets provide additional challenges for constructing reliable indices of stock abundance. After reviewing the use of general linear models to construct indices of annual stock abundance, potential biases which can arise due to the unequal and changing nature of the spatial distribution of fishing effort are examined and illustrated through the analysis of simulated data. Finally, some options are suggested for modelling catch rates in unfished strata and for accounting for the uncertainties in the stock and fishery dynamics which arise in the interpretation of spatially varying catch rate data.     

Analysing commercial catch and effort data from a Penaeid trawl fishery: A comparison of linear models, mixed models, and generalised estimating equations approaches

Statistical methods are often used to analyse commercial catch and effort data to provide standardised fishing effort and/or a relative index of fish abundance for input into stock assessment models. Achieving reliable results has proved difficult in Australia's Northern Prawn Fishery (NPF), due to a combination of such factors as the biological characteristics of the animals, some aspects of the fleet dynamics, and the changes in fishing technology. For this set of data, we compared four modelling approaches (linear models, mixed models, generalised estimating equations, and generalised linear models) with respect to the outcomes of the standardised fishing effort or the relative index of abundance. We also varied the number and form of vessel covariates in the models. Within a subset of data from this fishery, modelling correlation structures did not alter the conclusions from simpler statistical models. The random-effects models also yielded similar results. This is because the estimators are all consistent even if the correlation structure is mis-specified, and the data set is very large. However, the standard errors from different models differed, suggesting that different methods have different statistical efficiency. We suggest that there is value in modelling the variance function and the correlation structure, to make valid and efficient statistical inferences and gain insight into the data. We found that fishing power was separable from the indices of prawn abundance only when we offset the impact of vessel characteristics at assumed values from external sources. This may be due to the large degree of confounding within the data, and the extreme temporal changes in certain aspects of individual vessels, the fleet and the fleet dynamics.     

Influence of disc content on zone sizes obtained in standard disc diffusion antimicrobial susceptibility tests

The influence of disc content on the zone sizes produced was investigated using standard disc diffusion antimicrobial agent susceptibility test protocols. Replicate measurements were made of the zones of inhibition produced by discs containing 2-64 μg of oxytetracycline and oxolinic acid against Escherichia coli, Aeromonas salmonicida and Vibrio parahaemolyticus. Five replicate data sets were obtained for each of the six organism-agent combinations. Analysis of these 30 data sets demonstrated that they all showed a very high correlation (mean r² = 0.98 ± 0.009) with the predictions of best-fit equations specifying a linear relationship between log₁₀ of the disc content (μg) and the resultant zone sizes (mm). The mean value of the slopes of the best-fit straight-line equations was 9.8 ± 1.8. The overall similarity of these values for the slopes suggested that the quantitative relationship between disc content and zone size was, at least to a first approximation, independent of the organism or the agent involved in the test.     

Standardising the CPUE for the <Emphasis Type="Italic">Illex argentinus</Emphasis> fishery in the Southwest Atlantic

The catch per unit effort (CPUE) is a widely used index for assessing the abundance of exploited populations in fishery management. To obtain appropriate CPUE values, it is essential to standardise catch-effort data from fisheries. This task is particularly important for squid fisheries because squid generally have a short life-span and are vulnerable to environmental variability, and thus effective fishery management should take such factors into account. In this study, we analysed unit catches of paired vessels operating under similar fishing conditions to calculate their relative fishing power (RFP) in order to standardise the CPUE of the Taiwanese fleet jigging for Illex argentinus in the Southwest Atlantic. To evaluate the appropriateness of the method, we used a logbook dataset covering eleven years (1993–2003), in which 93.5% of the total catch during the period was included. The results indicate that 98.7% of the fishing effort can be standardised according to the estimated RFP. Compared to nominal CPUE, the standardised CPUE values projected an explainable temporal pattern, indicating an increasing trend in abundance from 1995 to 1999 and a subsequent sharp plunge from 1999 to 2003. However, the RFP was not related to apparent physical factors of the vessel, such as gross tonnage or vessel length. Our evaluations suggest that the RFP method is appropriate for standardising the CPUE, so that it can serve as an abundance index that reflects the annual recruitment size of the squid fishery, because the quality of the method can potentially take possible affecting factors into account in order to satisfy the general assumptions of standardisation criteria. However, the effects of varying the settings of parameters should be carefully examined prior to applying this standardisation method to other squid fisheries.