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排序方式: 共有652条查询结果,搜索用时 31 毫秒
1.
应用电子显微镜和X射线微分析技术,对貉受精过程和受精卵膜元素研究的结果表明,貉卵子的卵丘细胞具有吞噬和过滤功能;卵丘细胞间的精子顶体尚未发生囊泡化,而附着于透明带的精子发生了顶体反应,并以80°角穿入透明带,在其穿入的前方打开一个通道,最后穿过。穿过透明带的精子以赤道段或顶体后区同卵膜融合,并激发皮质颗粒释放,接着穿入卵内,最终发育成雌、雄原核。原核期受精卵质膜上具有高含量的钙,并呈集团分布,它在皮质颗粒胞吐释放中起着重要作用。 相似文献
2.
R. J. Zeyen W. M. Kruger M. F. Lyngkjr T. L. W. Carver 《Physiological and Molecular Plant Pathology》2002,61(6)
Barley, oat and wheat were used as both inappropriate hosts (IH) and appropriate hosts (AH) for three formae speciales of the fungus Blumeria graminis, the causal agent of powdery mildew disease. Treatment with either the glucose analog 2-deoxy-
-glucose (DDG) or with
-mannose dramatically suppressed penetration resistance in IH and to a much lesser extent in AH combinations. Other effects of DDG and
-mannose were strikingly dissimilar. DDG greatly reduced localized autofluorescence at fungal attack sites on epidermal cells, and prevented hypersensitive epidermal cell death (HR).
-mannose had little effect on autofluorescence or HR. DDG arrested the development of fungal haustoria and apparently prohibited biotrophy leading to secondary hyphae.
-mannose allowed haustorial development and functional biotrophy leading to the production of elongating secondary hyphae. This suggests that B. graminis is in some way capable of utilizing
-mannose as a carbon substrate. Results with IH combinations paralleled those of known mlo -barley responses to DDG and
-mannose. Results are discussed in relation to specific physiological processes known to be influenced by either DDG or by
-mannose, or by both compounds. 相似文献
3.
Y. J. Huang C. Toscano-Underwood B. D. L. Fitt † X. J. Hu A. M. Hall 《Plant pathology》2003,52(2):245-255
Ascospores of both A-group and B-group Leptosphaeria maculans germinated at temperatures from 5 to 20°C on leaves of oilseed rape. Germination of ascospores of both groups started 2 h after inoculation and percentage germination reached its maximum about 14 h after inoculation at all temperatures. Both the percentage of A-/B-group ascospores that had germinated after 24 h incubation and germ tube length increased with increasing temperature from 5 to 20°C. Germ tubes from B-group ascospores were longer than those from A-group ascospores at all temperatures, with the greatest difference at 20°C. Hyphae from ascospores of both groups penetrated the leaves predominantly through stomata, at temperatures from 5 to 20°C. A-group ascospores produced highly branched hyphae that grew tortuously, whereas B-group ascospores produced long, straight hyphae. The percentage of germinated ascospores that penetrated stomata increased with increasing temperature from 5 to 20°C and was greater for A-group than for B-group L. maculans after 40 h incubation. 相似文献
4.
瘦肉型猪(PIC344)精液碱性磷酸酶的分离纯化及部分性质研究 总被引:2,自引:0,他引:2
用正丁醇抽提、硫酸铵分级沉淀、DEAE SepharoseFF和SephacrylS 200柱层系,从瘦肉型猪(PIC344)精液中提取出碱性磷酸酶。纯化倍数11 46,比活为81 23U/mg蛋白。提取液经PAGE和SDS PAGE检测,呈现一条带。该酶相对分子质量为90 12KD,亚基分子质量为48 41KD,该酶为两个相同亚基组成。等电点为8 19,最适pH值9 5,最适温度为40℃,以磷酸苯二钠为底物测得Km值为3 98×10-4mol/L。经分析,Cu2 、Cd2 为酶的抑制剂,Ni2 、Ca2 、Mg2 为该酶的激活剂。选用CuSO4、Cd(NO3)2作酶的抑制类型判断,结果表明,CuSO4为非竞争性抑制,抑制常数为1 32×10-3mol/L,Cd(NO3)2为竞争性抑制,抑制常数为3 33×10-4mol/L。 相似文献
5.
Resistance to Botrytis cinerea in Solanum lycopersicoides is Dominant in Hybrids with Tomato, and Involves induced Hyphal Death 总被引:2,自引:0,他引:2
Rejane L. Guimarães Roger T. Chetelat Henrik U. Stotz 《European journal of plant pathology / European Foundation for Plant Pathology》2004,110(1):13-23
Botrytis cinerea causes gray mold disease and affects hundreds of plant species, including tomato (Lycopersicon esculentum). The wild nightshade, Solanum lycopersicoides, is cross compatible with tomato and is more resistant to B. cinerea, thus representing a potential source for crop improvement. Tests involving droplet inoculation of detached leaves and spray inoculation of entire seedlings demonstrated that resistance to B. cinerea varies among S. lycopersicoides accessions, with S. lycopersicoides LA2951 being the most resistant accession tested. Expression of resistance in the intergeneric hybrid (L. esculentum cv. 'VF36' × S. lycopersicoides LA2951) suggested that resistance is at least partially dominant in tomato. A green fluorescent protein-tagged B. cinerea strain was used for confocal microscopic comparison of infection in leaves of S. lycopersicoides and tomato. Even though S. lycopersicoides supported spore germination, there was evidence for hyphal lysis and death 3 days after inoculation, at a time when lesions were expanding on susceptible tomato plants. The reduced frequency of B. cinerea lesion spread on S. lycopersicoides explains why this fungus produced fewer spores in this wild nightshade than in tomato. 相似文献
6.
7.
鉴于侵彻引信实际作战环境的复杂多变性,设计一种半实物仿真系统对于引信综合性能的评估具有积极意义。首先重点分析了侵彻过载数据库的建立和侵彻引信的起爆控制模块的设计。为检验半实物仿真系统的实际工作能力,在现有的试验条件以及实现硬目标侵彻引信半实物仿真系统设计的基础之上,对引信的计时起爆控制模式进行了仿真试验研究。 相似文献
8.
Soil compaction, especially subsoil compaction, in agricultural fields has increased due to widespread use of heavy machines and intensification of vehicular traffic. Subsoil compaction changes the relative distribution of roots between soil layers and may restrict root development to the upper part of the soil profile, limiting water and mineral availability. This study investigated the direct effects of inter-row subsoiling, biological subsoiling and a combination of these two methods on soil penetration resistance, root length density, nitrogen uptake and yield. In field experiments with potatoes in 2013 and 2014, inter-row subsoiling (subsoiler) and biological subsoiling (preceding crops) were studied as two potential methods to reduce soil penetration resistance. Inter-row subsoiling was carried out post planting and the preceding crops were established one year, or in one case two years, prior to planting. Soil resistance was determined with a penetrometer three weeks after the potatoes were planted and root length density was measured after soil core sampling 2 months after emergence. Nitrogen uptake was determined in haulm (at haulm killing) and tubers (at harvest). Inter-row subsoiling had the greatest effect on soil penetration resistance, whereas biological subsoiling showed no effects. Root length density (RDL) in the combined treatment was higher than in the separate inter-row and biological subsoiling treatments and the control, whereas for the separate inter-row and biological subsoiling treatments, RLD was higher than in the control. Nitrogen uptake increased with inter-row subsoiling and was significantly higher than in the biological subsoiling and control treatments. However, in these experiments with a good supply of nutrients and water, no yield differences between any treatments were observed. 相似文献
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10.
指出了“禅”从印度传入中国,与中国的传统文化相结合产生了禅宗文化。艺术设计师们运用禅宗思想进行艺术设计,使禅意设计渗透到园林、服装、陶器、家居、广告、包装等许多艺术领域。分析了禅宗思想的精神内涵和掌握禅意设计的意境营造、物象型塑、符号联想等基本手法,提出了将禅意元素运用于艺术设计,可以将艺术作品推向更高的精神境界,并最终美化我们的生活空间。 相似文献