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IntroductionCystic echinococcosis (CE) is a chronic zoonotic disease caused by the larval stage of Echinococcus granulosus (E. granulosus), which affects domestic and wild carnivores as the definitive host and ungulates as intermediate hosts. In intermediate hosts, both Th1 and Th2 cells are involved in the immune responses to an echinoccocal infection. This study aimed to investigate production of IL-4, IL-10, and IFN-γ cytokines in peripheral blood mononuclear cells (PBMCs) of CE patients before and after surgical treatment.MethodsTo evaluate cytokine production in response to E. granulosus antigens, we investigated IL-4, IL-10, and IFN-γ production in PBMCs of 20 CE patients in response to hydatid cyst fluid antigen (HCF-Ag) before and after surgical treatment using ELISA.ResultsThe mean IL-4 production from HCF-Ag stimulated PBMCs was significantly decreased (p < 0.05), while IFN-γ was significantly increased in HCF-Ag stimulated PBMCs in patients after surgery (p = 0.005).Furthermore, our results showed that there is no significant difference between IL-10 production in patients before and after treatment (p = 0.562).ConclusionsOur data Indicated production of IL-4 in cultured PBMCs of CE patients stimulated with HCF-Ag was decreased significantly. While, production of IFN-γ was increased significantly in responses to HCF Ag after surgery. We concluded that the evaluation of IL-4 and IFN-γ in HCF-Ag stimulated PBMCs of CE patients should be considered as a useful marker in the follow up of patients with cystic echinococcosis. 相似文献
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为研究日粮中添加γ-氨基丁酸对热应激大鼠子代发育和抗氧化功能的影响,选用90日龄的Sprague-Dawley大鼠52只(雌性大鼠39只,雄性13只)进行试验,交配后27只受孕雌鼠随机分为三组,正常对照组,热应激组和GABA热应激组,仔鼠出生后1天,测定发育指标和肝脏抗氧化指标。结果显示,与正常对照组相比,热应激组新生雌鼠和雄鼠的尾长均显著降低(P<0.01);新生大鼠的肝脏谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)活性有所降低,丙二醛(MDA)含量有所升高,但差异均不显著。与热应激组相比,GABA热应激组新生雌鼠(P<0.01)和雄鼠(P<0.05)的尾长均显著提高;新生大鼠的肝脏MDA含量有所降低,GSH-Px、SOD活性有所升高,但差异均不显著。结果提示GABA一定程度上可改善孕期热应激条件下新生大鼠的抗应激能力。 相似文献
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γ-Oryzanol is a main oleophilic component in rice bran oil and has been well recognized as a good dietary supplement for human health, as well as having uses in industrial materials. japonica-type rice cultivars generally showed significantly higher contents of total γ-oryzanol in brown rice compared with indica-type cultivars, although within-group variation was significant. The objective of this study was to explore quantitative trait loci (QTLs) responsible for the difference in the γ-oryzanol content between japonica-type and indica-type rice cultivars, using recombinant inbred lines (RILs), backcross inbred lines (BILs), and corresponding chromosome segment substitution lines (CSSLs) derived from crosses between japonica-type and indica-type. Results from RILs and BILs showed that eight QTLs were detected with R2 from .09 to .16. Nine candidate regions for QTL were also suggested from corresponding CSSLs. These QTLs from RILs and BILs and the candidate regions from CSSLs were not overlapped, although one QTLs was mapped near the boundaries of the respective candidate region. At four QTLs and three candidate regions, alleles or segments from japonica-type caused higher contents than those from indica-type. On the other hand, at the other four QTLs and six candidate regions, alleles or segments from indica-type caused higher contents than those from japonica-type, which is a reverse result to the parental differences. This result strongly suggested that alleles with increasing effects on γ-oryzanol content could be accumulated not only from japonica-type but also from indica-type, leading to a potential for increase in γ-oryzanol content in future breeding programs. 相似文献
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以美国‘大速’不结球生菜为材料,在水培条件下采用浸种、叶面喷施、营养液添加3种方法,研究了不同浓度γ-氨基丁酸(GABA)对植株生长和营养品质的影响。结果表明:3种施用GABA的方法均可促进生菜的生长,并通过提高硝酸还原酶(NR)活性降低了体内硝酸盐含量,提高了生菜品质;但不同施用方法均存在浓度效应,其中浸种处理、叶面喷施、营养液添加GABA的浓度分别以5.00、100、2.50mmol/L效果较好,在最适宜浓度下对生长和品质指标综合分析认为,营养液添加2.50mmol/L GABA处理的效果最好。 相似文献
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为提高湘尖茶品质,采用~(60)Co-γ射线对湘尖茶进行不同剂量(0~10kGy)的辐照处理,研究了处理后湘尖茶主要化学组分含量和微生物含量的变化。结果表明:辐照处理对湘尖茶中水浸出物总量、咖啡碱和茶碱影响不显著;茶多酚、儿茶素总量、游离氨基酸总量、茶氨酸和可可碱含量分别在辐照剂量为4、6、4、2和10 kGy时达到最大值;2 kGy辐照剂量可以控制湘尖茶中菌落总数和霉菌数均在103以内。因此,综合考虑辐照成本和茶叶质量,可以采用2~6 kGy辐照处理来提高湘尖茶的品质。 相似文献
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