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冰淇淋中大肠菌群的传统检测方法繁琐且费时费力,不能适应现代化大型食品企业检测工作的需求。本研究将Soleris实时光电微生物检测技术引入到大肠菌群的快速检测中,得到了冰淇淋中大肠菌群检测标准曲线LogCFU=9.5175-0.854×DT(R2=0.9717);并用国标平板法对标准曲线进行了验证,结果表明Soleris法测得的结果与平板计数结果偏差在±0.5范围以内,具有较好的一致性,重复性验证结果RSD值在0.443%~0.501%之间。而与平板计数法相比,Soleris法具有快速的优点,可使冰淇淋产品放行时间缩短13~62h。本研究建立的大肠菌群Soleris检测法具有检测时间短、灵敏度高的优势,为冰淇淋产品的微生物检测提供了一种新的方法。 相似文献
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3M纸片检测大肠菌群法与国标快速检测法的比较研究 总被引:1,自引:0,他引:1
[目的]比较用3M纸片法与用快速检测国标法(MEFH)对不同样品大肠菌群的检测结果。[方法]以花生、小米、玉米、大米和花生油为检测样品,以Aliz-gal和MUGal肉汤为培养基,设大肠菌群污染的自然状态未知浓度和模拟培养已知浓度的两种处理,进行3M纸片法与MEFH法对大肠菌群的检测结果对比试验。[结果]3M法与MEFH中MPN法检测自然状态的5种样品的大肠菌群结果比较符合率为90%,3M纸片法和MEFH中的平板法检测结果无明显差异。3M纸片法未出现假阳性现象,证明了3M纸片法有很强的选择性。[结论]3M纸片法操作简便,适合企业现场食品卫生检测。 相似文献
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The mussel and fish-farming impact was investigated both in pelagic and sediment environments of Mar Piccolo in Taranto. We studied the yearly distribution of microbiological indicators (heterotrophic bacteria and coliforms), in relation to physical and chemical parameters of surface water at three different sites, characterized by mussel farms, fish cages and Citro springs. The heterotrophic bacteria and Clostridium perfrigens distribution were also examined in relation to organic content and granulometry of sediments. The urban sewages affected the quality of waters of the mussel site, by increasing faecal coliforms in the entire basin during summer. In the benthonic environment the aquaculture activity increased the bio-deposition of the sediment, causing changes in the abundance and the composition (heterotrophic bacteria/clostridia ratio) of microflora. A significant increase of heterotrophic bacteria in the warm season was observed. The clostridia proved to be a good indicator of impact of organic matter because they were significantly higher in the sites than the control stations. 相似文献
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